Mansonone G and its derivatives exhibit membrane permeabilizing activities against bacteria.
In an era where the rate of bacteria evolving to be resistant to clinically-used antibiotics far exceeds that of antibiotic discovery, the search for new sources of antibacterial agents has expanded tremendously. In recent years, interest in plant-based natural products as promising sources of antib...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2022-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0273614 |
| _version_ | 1798001009818075136 |
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| author | Htut Htut Htoo Nhung Ngo Thi Tuyet Kittiporn Nakprasit Chanat Aonbangkhen Vorrapon Chaikeeratisak Warinthorn Chavasiri Poochit Nonejuie |
| author_facet | Htut Htut Htoo Nhung Ngo Thi Tuyet Kittiporn Nakprasit Chanat Aonbangkhen Vorrapon Chaikeeratisak Warinthorn Chavasiri Poochit Nonejuie |
| author_sort | Htut Htut Htoo |
| collection | DOAJ |
| description | In an era where the rate of bacteria evolving to be resistant to clinically-used antibiotics far exceeds that of antibiotic discovery, the search for new sources of antibacterial agents has expanded tremendously. In recent years, interest in plant-based natural products as promising sources of antibacterial agents has taken an upward trend. Mansonones, botanically-derived naphthoqionones, having many uses in Asian traditional medicine-including anti-infective roles-have sparked interest as a possible source of antibacterial agents. Here, we show that mansonone G, extracted from Mansonia gagei Drumm. heartwoods, possessed antibacterial activities towards Bacillus subtilis, Staphylococcus aureus and Escherichia coli lptD4213, inhibiting the growth of the bacteria at 15.6 μM, 62.5 μM and 125 μM, respectively. Fourteen derivatives of mansonone G were synthesized successfully and were found to have a similar antibacterial spectrum to that of the parent compound, with some derivatives possessing improved antibacterial activities. Bacterial cytological profiling analysis showed that mansonone G harbors membrane permeabilizing activities against B. subtilis and E. coli lptD4213. Temporal analysis of SYTOX Green staining among individual cells showed that mansonone G rapidly permeabilized bacterial membrane within 10 min, with SYTOX Green intensity reaching 13-fold above that of the control. Collectively, these findings highlight the importance of mansonone G and its derivatives as potential antibacterial agents, paving the way for further modifications in order to improve their antibacterial spectrum. |
| first_indexed | 2024-04-11T11:29:14Z |
| format | Article |
| id | doaj.art-59f2f4290f384524b60e1972e3b09aa7 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-11T11:29:14Z |
| publishDate | 2022-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-59f2f4290f384524b60e1972e3b09aa72022-12-22T04:26:11ZengPublic Library of Science (PLoS)PLoS ONE1932-62032022-01-01179e027361410.1371/journal.pone.0273614Mansonone G and its derivatives exhibit membrane permeabilizing activities against bacteria.Htut Htut HtooNhung Ngo Thi TuyetKittiporn NakprasitChanat AonbangkhenVorrapon ChaikeeratisakWarinthorn ChavasiriPoochit NonejuieIn an era where the rate of bacteria evolving to be resistant to clinically-used antibiotics far exceeds that of antibiotic discovery, the search for new sources of antibacterial agents has expanded tremendously. In recent years, interest in plant-based natural products as promising sources of antibacterial agents has taken an upward trend. Mansonones, botanically-derived naphthoqionones, having many uses in Asian traditional medicine-including anti-infective roles-have sparked interest as a possible source of antibacterial agents. Here, we show that mansonone G, extracted from Mansonia gagei Drumm. heartwoods, possessed antibacterial activities towards Bacillus subtilis, Staphylococcus aureus and Escherichia coli lptD4213, inhibiting the growth of the bacteria at 15.6 μM, 62.5 μM and 125 μM, respectively. Fourteen derivatives of mansonone G were synthesized successfully and were found to have a similar antibacterial spectrum to that of the parent compound, with some derivatives possessing improved antibacterial activities. Bacterial cytological profiling analysis showed that mansonone G harbors membrane permeabilizing activities against B. subtilis and E. coli lptD4213. Temporal analysis of SYTOX Green staining among individual cells showed that mansonone G rapidly permeabilized bacterial membrane within 10 min, with SYTOX Green intensity reaching 13-fold above that of the control. Collectively, these findings highlight the importance of mansonone G and its derivatives as potential antibacterial agents, paving the way for further modifications in order to improve their antibacterial spectrum.https://doi.org/10.1371/journal.pone.0273614 |
| spellingShingle | Htut Htut Htoo Nhung Ngo Thi Tuyet Kittiporn Nakprasit Chanat Aonbangkhen Vorrapon Chaikeeratisak Warinthorn Chavasiri Poochit Nonejuie Mansonone G and its derivatives exhibit membrane permeabilizing activities against bacteria. PLoS ONE |
| title | Mansonone G and its derivatives exhibit membrane permeabilizing activities against bacteria. |
| title_full | Mansonone G and its derivatives exhibit membrane permeabilizing activities against bacteria. |
| title_fullStr | Mansonone G and its derivatives exhibit membrane permeabilizing activities against bacteria. |
| title_full_unstemmed | Mansonone G and its derivatives exhibit membrane permeabilizing activities against bacteria. |
| title_short | Mansonone G and its derivatives exhibit membrane permeabilizing activities against bacteria. |
| title_sort | mansonone g and its derivatives exhibit membrane permeabilizing activities against bacteria |
| url | https://doi.org/10.1371/journal.pone.0273614 |
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