Association between Microsatellite Instability Status and Peri-Operative Release of Circulating Tumour Cells in Colorectal Cancer
Microsatellite instability (MSI) in colorectal cancer (CRC) is a marker of immunogenicity and is associated with an increased abundance of tumour infiltrating lymphocytes (TILs). In this subgroup of colorectal cancer, it is unknown if these characteristics translate into a measurable difference in c...
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MDPI AG
2020-02-01
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| Series: | Cells |
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| Online Access: | https://www.mdpi.com/2073-4409/9/2/425 |
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| author | James W. T. Toh Stephanie H. Lim Scott MacKenzie Paul de Souza Les Bokey Pierre Chapuis Kevin J. Spring |
| author_facet | James W. T. Toh Stephanie H. Lim Scott MacKenzie Paul de Souza Les Bokey Pierre Chapuis Kevin J. Spring |
| author_sort | James W. T. Toh |
| collection | DOAJ |
| description | Microsatellite instability (MSI) in colorectal cancer (CRC) is a marker of immunogenicity and is associated with an increased abundance of tumour infiltrating lymphocytes (TILs). In this subgroup of colorectal cancer, it is unknown if these characteristics translate into a measurable difference in circulating tumour cell (CTC) release into peripheral circulation. This is the first study to compare MSI status with the prevalence of circulating CTCs in the peri-operative colorectal surgery setting. For this purpose, 20 patients who underwent CRC surgery with curative intent were enrolled in the study, and peripheral venous blood was collected at pre- (t1), intra- (t2), immediately post-operative (t3), and 14−16 h post-operative (t4) time points. Of these, one patient was excluded due to insufficient blood sample. CTCs were isolated from 19 patients using the Isoflux<sup>TM</sup> system, and the data were analysed using the STATA statistical package. CTC number was presented as the mean values, and comparisons were made using the Student <i>t</i>-test. There was a trend toward increased CTC presence in the MSI-high (H) CRC group, but this was not statistically significant. In addition, a Poisson regression was performed adjusting for stage (I-IV). This demonstrated no significant difference between the two MSI groups for pre-operative time point t1. However, time points t2, t3, and t4 were associated with increased CTC presence for MSI-H CRCs. In conclusion, there was a trend toward increased CTC release pre-, intra-, and post-operatively in MSI-H CRCs, but this was only statistically significant intra-operatively. When adjusting for stage, MSI-H was associated with an increase in CTC numbers intra-operatively and post-operatively, but not pre-operatively. |
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| last_indexed | 2024-03-12T19:00:16Z |
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| spelling | doaj.art-59f36504d4cb45d58b5dacf4c2ecf3472023-08-02T06:39:38ZengMDPI AGCells2073-44092020-02-019242510.3390/cells9020425cells9020425Association between Microsatellite Instability Status and Peri-Operative Release of Circulating Tumour Cells in Colorectal CancerJames W. T. Toh0Stephanie H. Lim1Scott MacKenzie2Paul de Souza3Les Bokey4Pierre Chapuis5Kevin J. Spring6Medical Oncology, Ingham Institute of Applied Research, School of Medicine, Western Sydney University and SWS Clinical School, UNSW Sydney 2170, NSW, AustraliaMedical Oncology, Ingham Institute of Applied Research, School of Medicine, Western Sydney University and SWS Clinical School, UNSW Sydney 2170, NSW, AustraliaLiverpool Clinical School, Western Sydney University, Sydney 2170, AustraliaMedical Oncology, Ingham Institute of Applied Research, School of Medicine, Western Sydney University and SWS Clinical School, UNSW Sydney 2170, NSW, AustraliaLiverpool Clinical School, Western Sydney University, Sydney 2170, AustraliaDepartment of Colorectal Surgery, Concord Hospital and Discipline of Surgery, Sydney Medical School, University of Sydney, Sydney 2137, AustraliaMedical Oncology, Ingham Institute of Applied Research, School of Medicine, Western Sydney University and SWS Clinical School, UNSW Sydney 2170, NSW, AustraliaMicrosatellite instability (MSI) in colorectal cancer (CRC) is a marker of immunogenicity and is associated with an increased abundance of tumour infiltrating lymphocytes (TILs). In this subgroup of colorectal cancer, it is unknown if these characteristics translate into a measurable difference in circulating tumour cell (CTC) release into peripheral circulation. This is the first study to compare MSI status with the prevalence of circulating CTCs in the peri-operative colorectal surgery setting. For this purpose, 20 patients who underwent CRC surgery with curative intent were enrolled in the study, and peripheral venous blood was collected at pre- (t1), intra- (t2), immediately post-operative (t3), and 14−16 h post-operative (t4) time points. Of these, one patient was excluded due to insufficient blood sample. CTCs were isolated from 19 patients using the Isoflux<sup>TM</sup> system, and the data were analysed using the STATA statistical package. CTC number was presented as the mean values, and comparisons were made using the Student <i>t</i>-test. There was a trend toward increased CTC presence in the MSI-high (H) CRC group, but this was not statistically significant. In addition, a Poisson regression was performed adjusting for stage (I-IV). This demonstrated no significant difference between the two MSI groups for pre-operative time point t1. However, time points t2, t3, and t4 were associated with increased CTC presence for MSI-H CRCs. In conclusion, there was a trend toward increased CTC release pre-, intra-, and post-operatively in MSI-H CRCs, but this was only statistically significant intra-operatively. When adjusting for stage, MSI-H was associated with an increase in CTC numbers intra-operatively and post-operatively, but not pre-operatively.https://www.mdpi.com/2073-4409/9/2/425circulating tumour cellscolorectal cancercolorectal surgerymicrosatellite instability |
| spellingShingle | James W. T. Toh Stephanie H. Lim Scott MacKenzie Paul de Souza Les Bokey Pierre Chapuis Kevin J. Spring Association between Microsatellite Instability Status and Peri-Operative Release of Circulating Tumour Cells in Colorectal Cancer Cells circulating tumour cells colorectal cancer colorectal surgery microsatellite instability |
| title | Association between Microsatellite Instability Status and Peri-Operative Release of Circulating Tumour Cells in Colorectal Cancer |
| title_full | Association between Microsatellite Instability Status and Peri-Operative Release of Circulating Tumour Cells in Colorectal Cancer |
| title_fullStr | Association between Microsatellite Instability Status and Peri-Operative Release of Circulating Tumour Cells in Colorectal Cancer |
| title_full_unstemmed | Association between Microsatellite Instability Status and Peri-Operative Release of Circulating Tumour Cells in Colorectal Cancer |
| title_short | Association between Microsatellite Instability Status and Peri-Operative Release of Circulating Tumour Cells in Colorectal Cancer |
| title_sort | association between microsatellite instability status and peri operative release of circulating tumour cells in colorectal cancer |
| topic | circulating tumour cells colorectal cancer colorectal surgery microsatellite instability |
| url | https://www.mdpi.com/2073-4409/9/2/425 |
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