Growth Kinetics, Purification and Characterization of α-amylase Produced from Bacillus licheniformis DSM-1969 using Lignocellulosic Banana Waste as an Elicitor
In this study, banana waste was used to investigate its elicitation potential for induced production of α-amylase from Bacillus licheniformis DSM-1969. Initially, six different media were investigated to select the composition with optimal yield. A comparison of the fermentations in the stirred ferm...
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North Carolina State University
2014-09-01
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| Series: | BioResources |
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| Online Access: | http://ojs.cnr.ncsu.edu/index.php/BioRes/article/view/BioRes_09_4_6791_Bashir_Growth_Kinetics_Purification_Bacillus |
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| author | Rashida Bashir Quratulain Syed Muhammad Waseem Mumtaz Farooq Anwar Nazamid Saari Ahmad Adnan |
| author_facet | Rashida Bashir Quratulain Syed Muhammad Waseem Mumtaz Farooq Anwar Nazamid Saari Ahmad Adnan |
| author_sort | Rashida Bashir |
| collection | DOAJ |
| description | In this study, banana waste was used to investigate its elicitation potential for induced production of α-amylase from Bacillus licheniformis DSM-1969. Initially, six different media were investigated to select the composition with optimal yield. A comparison of the fermentations in the stirred fermenter or shake flasks revealed that B. licheniformis DSM-1969 was more active to synthesize α-amylase in the fermenter as compared to the shake flask. In the shake flask during the exponential phase, the specific growth rate, generation time, and number of generations were 0.19 h-1, 3.48 h-1, and 5.16 h-1, respectively, whereas in the stirred fermenter the above values were 0.3 h-1, 2.31 h-1 and 5.21 h-1, respectively. A significant difference was recorded in the specific substrate uptake rate and biomass growth yield during the exponential phase in the stirred fermenter in comparison to the shake flask. The enzyme was purified by ion-exchange chromatography using fast protein liquid chromatography (FPLC). α-amylase was purified 3.9 fold with a specific activity of 38.8 U/mg and molecular weight of 62 kDa. Characterization revealed that purified α-amylase remained stable over a broad pH and temperature range as compared to the crude enzyme. Activity of this novel extra thermo-stable α-amylase was stimulated to variable extents by Zn2+, Co2+, and Mn2+, whereas EDTA and Hg2+ showed inhibitory effects. |
| first_indexed | 2024-12-17T13:02:36Z |
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| id | doaj.art-59f81a7c12a64f69be5ecf1baf887147 |
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| issn | 1930-2126 1930-2126 |
| language | English |
| last_indexed | 2024-12-17T13:02:36Z |
| publishDate | 2014-09-01 |
| publisher | North Carolina State University |
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| series | BioResources |
| spelling | doaj.art-59f81a7c12a64f69be5ecf1baf8871472022-12-21T21:47:20ZengNorth Carolina State UniversityBioResources1930-21261930-21262014-09-01946791680410.15376/biores.9.4.6791-6804Growth Kinetics, Purification and Characterization of α-amylase Produced from Bacillus licheniformis DSM-1969 using Lignocellulosic Banana Waste as an ElicitorRashida Bashir0Quratulain Syed1Muhammad Waseem Mumtaz2Farooq Anwar3Nazamid Saari4Ahmad Adnan5Department of Chemistry, Government College University Lahore, Lahore, Pakistan; PakistanDepartment of Chemistry, Government College University Lahore, Lahore, Pakistan; PakistanDepartment of Chemistry, University of Gujrat, Gujrat, Pakistan; PakistanDepartment of Chemistry, University of Sargodha, Pakistan; PakistanFaculties of Food Science and Technology, University Putra Malaysia, UPM-43400 Serdang, Selangor, Malaysia; MalaysiaDepartment of Chemistry, Government College University Lahore, Lahore, Pakistan; PakistanIn this study, banana waste was used to investigate its elicitation potential for induced production of α-amylase from Bacillus licheniformis DSM-1969. Initially, six different media were investigated to select the composition with optimal yield. A comparison of the fermentations in the stirred fermenter or shake flasks revealed that B. licheniformis DSM-1969 was more active to synthesize α-amylase in the fermenter as compared to the shake flask. In the shake flask during the exponential phase, the specific growth rate, generation time, and number of generations were 0.19 h-1, 3.48 h-1, and 5.16 h-1, respectively, whereas in the stirred fermenter the above values were 0.3 h-1, 2.31 h-1 and 5.21 h-1, respectively. A significant difference was recorded in the specific substrate uptake rate and biomass growth yield during the exponential phase in the stirred fermenter in comparison to the shake flask. The enzyme was purified by ion-exchange chromatography using fast protein liquid chromatography (FPLC). α-amylase was purified 3.9 fold with a specific activity of 38.8 U/mg and molecular weight of 62 kDa. Characterization revealed that purified α-amylase remained stable over a broad pH and temperature range as compared to the crude enzyme. Activity of this novel extra thermo-stable α-amylase was stimulated to variable extents by Zn2+, Co2+, and Mn2+, whereas EDTA and Hg2+ showed inhibitory effects.http://ojs.cnr.ncsu.edu/index.php/BioRes/article/view/BioRes_09_4_6791_Bashir_Growth_Kinetics_Purification_BacillusGreen biotechnologyBanana wasteα-amylaseElicitationPurificationCharacterization |
| spellingShingle | Rashida Bashir Quratulain Syed Muhammad Waseem Mumtaz Farooq Anwar Nazamid Saari Ahmad Adnan Growth Kinetics, Purification and Characterization of α-amylase Produced from Bacillus licheniformis DSM-1969 using Lignocellulosic Banana Waste as an Elicitor BioResources Green biotechnology Banana waste α-amylase Elicitation Purification Characterization |
| title | Growth Kinetics, Purification and Characterization of α-amylase Produced from Bacillus licheniformis DSM-1969 using Lignocellulosic Banana Waste as an Elicitor |
| title_full | Growth Kinetics, Purification and Characterization of α-amylase Produced from Bacillus licheniformis DSM-1969 using Lignocellulosic Banana Waste as an Elicitor |
| title_fullStr | Growth Kinetics, Purification and Characterization of α-amylase Produced from Bacillus licheniformis DSM-1969 using Lignocellulosic Banana Waste as an Elicitor |
| title_full_unstemmed | Growth Kinetics, Purification and Characterization of α-amylase Produced from Bacillus licheniformis DSM-1969 using Lignocellulosic Banana Waste as an Elicitor |
| title_short | Growth Kinetics, Purification and Characterization of α-amylase Produced from Bacillus licheniformis DSM-1969 using Lignocellulosic Banana Waste as an Elicitor |
| title_sort | growth kinetics purification and characterization of α amylase produced from bacillus licheniformis dsm 1969 using lignocellulosic banana waste as an elicitor |
| topic | Green biotechnology Banana waste α-amylase Elicitation Purification Characterization |
| url | http://ojs.cnr.ncsu.edu/index.php/BioRes/article/view/BioRes_09_4_6791_Bashir_Growth_Kinetics_Purification_Bacillus |
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