Quantum-dot-based immunochromatographic assay for total IgE in human serum.
To rapidly quantify total immunoglobulin E levels in human serum, we developed a novel quantum-dot-based immunochromatographic assay that employs digital recording of fluorescence. It can detect IgE levels of 5-1000 kU/L, with a coefficient of variation ranging from 2.0 to 9.5%. The assay can be pro...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2013-01-01
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| Series: | PLoS ONE |
| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24204841/?tool=EBI |
| _version_ | 1818338040072896512 |
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| author | Anna N Berlina Nadezhda A Taranova Anatoly V Zherdev Mikhail N Sankov Igor V Andreev Alexandr I Martynov Boris B Dzantiev |
| author_facet | Anna N Berlina Nadezhda A Taranova Anatoly V Zherdev Mikhail N Sankov Igor V Andreev Alexandr I Martynov Boris B Dzantiev |
| author_sort | Anna N Berlina |
| collection | DOAJ |
| description | To rapidly quantify total immunoglobulin E levels in human serum, we developed a novel quantum-dot-based immunochromatographic assay that employs digital recording of fluorescence. It can detect IgE levels of 5-1000 kU/L, with a coefficient of variation ranging from 2.0 to 9.5%. The assay can be processed in 10 min. The developed assay was tested on 95 serum samples. The correlation coefficient between the IgE values obtained by the proposed assay and those obtained by a commercial ELISA kit was 0.9884. Our assay thus shows promise as a new diagnostic tool for IgE detection. |
| first_indexed | 2024-12-13T15:04:47Z |
| format | Article |
| id | doaj.art-59f98cb656464b3bb81b7f829b5ab669 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-13T15:04:47Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-59f98cb656464b3bb81b7f829b5ab6692022-12-21T23:41:01ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-01810e7748510.1371/journal.pone.0077485Quantum-dot-based immunochromatographic assay for total IgE in human serum.Anna N BerlinaNadezhda A TaranovaAnatoly V ZherdevMikhail N SankovIgor V AndreevAlexandr I MartynovBoris B DzantievTo rapidly quantify total immunoglobulin E levels in human serum, we developed a novel quantum-dot-based immunochromatographic assay that employs digital recording of fluorescence. It can detect IgE levels of 5-1000 kU/L, with a coefficient of variation ranging from 2.0 to 9.5%. The assay can be processed in 10 min. The developed assay was tested on 95 serum samples. The correlation coefficient between the IgE values obtained by the proposed assay and those obtained by a commercial ELISA kit was 0.9884. Our assay thus shows promise as a new diagnostic tool for IgE detection.https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24204841/?tool=EBI |
| spellingShingle | Anna N Berlina Nadezhda A Taranova Anatoly V Zherdev Mikhail N Sankov Igor V Andreev Alexandr I Martynov Boris B Dzantiev Quantum-dot-based immunochromatographic assay for total IgE in human serum. PLoS ONE |
| title | Quantum-dot-based immunochromatographic assay for total IgE in human serum. |
| title_full | Quantum-dot-based immunochromatographic assay for total IgE in human serum. |
| title_fullStr | Quantum-dot-based immunochromatographic assay for total IgE in human serum. |
| title_full_unstemmed | Quantum-dot-based immunochromatographic assay for total IgE in human serum. |
| title_short | Quantum-dot-based immunochromatographic assay for total IgE in human serum. |
| title_sort | quantum dot based immunochromatographic assay for total ige in human serum |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24204841/?tool=EBI |
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