PCN-224 Nanoparticle/Polyacrylonitrile Nanofiber Membrane for Light-Driven Bacterial Inactivation
Increasing issues of pathogen drug resistance and spreading pose a serious threat to the ability to treat common infectious diseases, which encourages people to explore effective technology to meet the challenge. Photodynamic antibacterial inactivation (aPDI) is being explored for inactivating patho...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-11-01
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| Series: | Nanomaterials |
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| Online Access: | https://www.mdpi.com/2079-4991/11/12/3162 |
| _version_ | 1797502046528602112 |
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| author | Xiaolin Nie Shuanglin Wu Tanveer Hussain Qufu Wei |
| author_facet | Xiaolin Nie Shuanglin Wu Tanveer Hussain Qufu Wei |
| author_sort | Xiaolin Nie |
| collection | DOAJ |
| description | Increasing issues of pathogen drug resistance and spreading pose a serious threat to the ability to treat common infectious diseases, which encourages people to explore effective technology to meet the challenge. Photodynamic antibacterial inactivation (aPDI) is being explored for inactivating pathogens, which could be used as a novel approach to prevent this threat. Here, porphyrin-embedded MOF material (PCN-224) with photodynamic effect was synthesized, then the PCN-224 nanoparticles (NPs) were embedded into PAN nanofibers with an electrospinning process (PAN-PCN nanofiber membrane). On the one hand, polyacrylonitrile (PAN) nanofibers help to improve the stability of PCN-224 NPs, which could avoid their leakage. On the other, the PAN nanofibers are used as a support material to load bactericidal PCN-224 NPs, realizing recycling after bacterial elimination. An antibacterial photodynamic inactivation (aPDI) study demonstrated that the PAN-PCN 0.6% nanofiber membrane processed 3.00 log unit elimination towards a <i>E. coli</i> bacterial strain and 4.70 log unit towards a <i>S. aureus</i> strain under illumination. A mechanism study revealed that this efficient bacterial elimination was due to singlet oxygen (<sup>1</sup>O<sub>2</sub>). Although the materials are highly phototoxic, an MTT assay showed that the as fabricated nanofiber membranes had good biocompatibility in the dark, and the cell survival rates were all above 85%. Taken together, this work provided an application prospect of nanofibers with an aPDI effect to deal with the issues of pathogen drug resistance and spreading. |
| first_indexed | 2024-03-10T03:27:15Z |
| format | Article |
| id | doaj.art-59fd409cad634b54b73848292f6aecdd |
| institution | Directory Open Access Journal |
| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-03-10T03:27:15Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nanomaterials |
| spelling | doaj.art-59fd409cad634b54b73848292f6aecdd2023-11-23T09:48:42ZengMDPI AGNanomaterials2079-49912021-11-011112316210.3390/nano11123162PCN-224 Nanoparticle/Polyacrylonitrile Nanofiber Membrane for Light-Driven Bacterial InactivationXiaolin Nie0Shuanglin Wu1Tanveer Hussain2Qufu Wei3Key Laboratory of Eco-Textiles, Ministry of Education, Jiangnan University, Wuxi 214122, ChinaKey Laboratory of Eco-Textiles, Ministry of Education, Jiangnan University, Wuxi 214122, ChinaTextile Processing Department, Faculty of Engineering & Technology, National Textile University, Faisalabad 37610, PakistanKey Laboratory of Eco-Textiles, Ministry of Education, Jiangnan University, Wuxi 214122, ChinaIncreasing issues of pathogen drug resistance and spreading pose a serious threat to the ability to treat common infectious diseases, which encourages people to explore effective technology to meet the challenge. Photodynamic antibacterial inactivation (aPDI) is being explored for inactivating pathogens, which could be used as a novel approach to prevent this threat. Here, porphyrin-embedded MOF material (PCN-224) with photodynamic effect was synthesized, then the PCN-224 nanoparticles (NPs) were embedded into PAN nanofibers with an electrospinning process (PAN-PCN nanofiber membrane). On the one hand, polyacrylonitrile (PAN) nanofibers help to improve the stability of PCN-224 NPs, which could avoid their leakage. On the other, the PAN nanofibers are used as a support material to load bactericidal PCN-224 NPs, realizing recycling after bacterial elimination. An antibacterial photodynamic inactivation (aPDI) study demonstrated that the PAN-PCN 0.6% nanofiber membrane processed 3.00 log unit elimination towards a <i>E. coli</i> bacterial strain and 4.70 log unit towards a <i>S. aureus</i> strain under illumination. A mechanism study revealed that this efficient bacterial elimination was due to singlet oxygen (<sup>1</sup>O<sub>2</sub>). Although the materials are highly phototoxic, an MTT assay showed that the as fabricated nanofiber membranes had good biocompatibility in the dark, and the cell survival rates were all above 85%. Taken together, this work provided an application prospect of nanofibers with an aPDI effect to deal with the issues of pathogen drug resistance and spreading.https://www.mdpi.com/2079-4991/11/12/3162electrospinningantibacterial photodynamic inactivationPCN-224polyacrylonitrilesinglet oxygen |
| spellingShingle | Xiaolin Nie Shuanglin Wu Tanveer Hussain Qufu Wei PCN-224 Nanoparticle/Polyacrylonitrile Nanofiber Membrane for Light-Driven Bacterial Inactivation Nanomaterials electrospinning antibacterial photodynamic inactivation PCN-224 polyacrylonitrile singlet oxygen |
| title | PCN-224 Nanoparticle/Polyacrylonitrile Nanofiber Membrane for Light-Driven Bacterial Inactivation |
| title_full | PCN-224 Nanoparticle/Polyacrylonitrile Nanofiber Membrane for Light-Driven Bacterial Inactivation |
| title_fullStr | PCN-224 Nanoparticle/Polyacrylonitrile Nanofiber Membrane for Light-Driven Bacterial Inactivation |
| title_full_unstemmed | PCN-224 Nanoparticle/Polyacrylonitrile Nanofiber Membrane for Light-Driven Bacterial Inactivation |
| title_short | PCN-224 Nanoparticle/Polyacrylonitrile Nanofiber Membrane for Light-Driven Bacterial Inactivation |
| title_sort | pcn 224 nanoparticle polyacrylonitrile nanofiber membrane for light driven bacterial inactivation |
| topic | electrospinning antibacterial photodynamic inactivation PCN-224 polyacrylonitrile singlet oxygen |
| url | https://www.mdpi.com/2079-4991/11/12/3162 |
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