Establishment of a Nipah Virus Disease Model in Hamsters, including a Comparison of Intranasal and Intraperitoneal Routes of Challenge
Nipah virus (NiV) is an emerging pathogen that can cause severe respiratory illness and encephalitis in humans. The main reservoir is fruit bats, distributed across a large geographical area that includes Australia, Southeast Asia, and Africa. Incursion into humans is widely reported through exposur...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-07-01
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| Series: | Pathogens |
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| Online Access: | https://www.mdpi.com/2076-0817/12/8/976 |
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| author | Stephen Findlay-Wilson Lucy Flett Francisco J. Salguero Ines Ruedas-Torres Susan Fotheringham Linda Easterbrook Victoria Graham Stuart Dowall |
| author_facet | Stephen Findlay-Wilson Lucy Flett Francisco J. Salguero Ines Ruedas-Torres Susan Fotheringham Linda Easterbrook Victoria Graham Stuart Dowall |
| author_sort | Stephen Findlay-Wilson |
| collection | DOAJ |
| description | Nipah virus (NiV) is an emerging pathogen that can cause severe respiratory illness and encephalitis in humans. The main reservoir is fruit bats, distributed across a large geographical area that includes Australia, Southeast Asia, and Africa. Incursion into humans is widely reported through exposure of infected pigs, ingestion of contaminated food, or through contact with an infected person. With no approved treatments or vaccines, NiV poses a threat to human public health and has epidemic potential. To aid with the assessment of emerging interventions being developed, an expansion of preclinical testing capability is required. Given variations in the model parameters observed in different sites during establishment, optimisation of challenge routes and doses is required. Upon evaluating the hamster model, an intranasal route of challenge was compared with intraperitoneal delivery, demonstrating a more rapid dissemination to wider tissues in the latter. A dose effect was observed between those causing respiratory illness and those resulting in neurological disease. The data demonstrate the successful establishment of the hamster model of NiV disease for subsequent use in the evaluation of vaccines and antivirals. |
| first_indexed | 2024-03-10T23:40:32Z |
| format | Article |
| id | doaj.art-5a003aefeb2c4e029f510a3bf90052d6 |
| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-03-10T23:40:32Z |
| publishDate | 2023-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pathogens |
| spelling | doaj.art-5a003aefeb2c4e029f510a3bf90052d62023-11-19T02:31:37ZengMDPI AGPathogens2076-08172023-07-0112897610.3390/pathogens12080976Establishment of a Nipah Virus Disease Model in Hamsters, including a Comparison of Intranasal and Intraperitoneal Routes of ChallengeStephen Findlay-Wilson0Lucy Flett1Francisco J. Salguero2Ines Ruedas-Torres3Susan Fotheringham4Linda Easterbrook5Victoria Graham6Stuart Dowall7United Kingdom Health Security Agency (UKHSA), Porton Down, Salisbury SP4 0JG, UKUnited Kingdom Health Security Agency (UKHSA), Porton Down, Salisbury SP4 0JG, UKUnited Kingdom Health Security Agency (UKHSA), Porton Down, Salisbury SP4 0JG, UKUnited Kingdom Health Security Agency (UKHSA), Porton Down, Salisbury SP4 0JG, UKUnited Kingdom Health Security Agency (UKHSA), Porton Down, Salisbury SP4 0JG, UKUnited Kingdom Health Security Agency (UKHSA), Porton Down, Salisbury SP4 0JG, UKUnited Kingdom Health Security Agency (UKHSA), Porton Down, Salisbury SP4 0JG, UKUnited Kingdom Health Security Agency (UKHSA), Porton Down, Salisbury SP4 0JG, UKNipah virus (NiV) is an emerging pathogen that can cause severe respiratory illness and encephalitis in humans. The main reservoir is fruit bats, distributed across a large geographical area that includes Australia, Southeast Asia, and Africa. Incursion into humans is widely reported through exposure of infected pigs, ingestion of contaminated food, or through contact with an infected person. With no approved treatments or vaccines, NiV poses a threat to human public health and has epidemic potential. To aid with the assessment of emerging interventions being developed, an expansion of preclinical testing capability is required. Given variations in the model parameters observed in different sites during establishment, optimisation of challenge routes and doses is required. Upon evaluating the hamster model, an intranasal route of challenge was compared with intraperitoneal delivery, demonstrating a more rapid dissemination to wider tissues in the latter. A dose effect was observed between those causing respiratory illness and those resulting in neurological disease. The data demonstrate the successful establishment of the hamster model of NiV disease for subsequent use in the evaluation of vaccines and antivirals.https://www.mdpi.com/2076-0817/12/8/976Nipahmodelintranasalinterperitonealchallengeefficacy |
| spellingShingle | Stephen Findlay-Wilson Lucy Flett Francisco J. Salguero Ines Ruedas-Torres Susan Fotheringham Linda Easterbrook Victoria Graham Stuart Dowall Establishment of a Nipah Virus Disease Model in Hamsters, including a Comparison of Intranasal and Intraperitoneal Routes of Challenge Pathogens Nipah model intranasal interperitoneal challenge efficacy |
| title | Establishment of a Nipah Virus Disease Model in Hamsters, including a Comparison of Intranasal and Intraperitoneal Routes of Challenge |
| title_full | Establishment of a Nipah Virus Disease Model in Hamsters, including a Comparison of Intranasal and Intraperitoneal Routes of Challenge |
| title_fullStr | Establishment of a Nipah Virus Disease Model in Hamsters, including a Comparison of Intranasal and Intraperitoneal Routes of Challenge |
| title_full_unstemmed | Establishment of a Nipah Virus Disease Model in Hamsters, including a Comparison of Intranasal and Intraperitoneal Routes of Challenge |
| title_short | Establishment of a Nipah Virus Disease Model in Hamsters, including a Comparison of Intranasal and Intraperitoneal Routes of Challenge |
| title_sort | establishment of a nipah virus disease model in hamsters including a comparison of intranasal and intraperitoneal routes of challenge |
| topic | Nipah model intranasal interperitoneal challenge efficacy |
| url | https://www.mdpi.com/2076-0817/12/8/976 |
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