Degradation Enhancement as the Mechanism of Resistance to Imazethapyr in Barnyardgrass
ABSTRACT: Non-target site (NTS) herbicide resistance by degradation enhancement is an increasing problem in several weeds around the world. In this study, the occurrence of degradation enhancement by cytochrome P450 monooxygenases (cytP450) was evaluated as the mechanism of resistance to imazethapyr...
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira da Ciência das Plantas Daninhas
2018-10-01
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| Series: | Planta Daninha |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582018000100317&tlng=en |
| _version_ | 1818904007553318912 |
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| author | G. DALAZEN A. PISONI R.S. RAFAELI A. MEROTTO JR. |
| author_facet | G. DALAZEN A. PISONI R.S. RAFAELI A. MEROTTO JR. |
| author_sort | G. DALAZEN |
| collection | DOAJ |
| description | ABSTRACT: Non-target site (NTS) herbicide resistance by degradation enhancement is an increasing problem in several weeds around the world. In this study, the occurrence of degradation enhancement by cytochrome P450 monooxygenases (cytP450) was evaluated as the mechanism of resistance to imazethapyr in barnyardgrass. The cytP450 inhibitors malathion and piperonyl butoxide (PBO) and the inducer naphthalic anhydride (NA), applied in mixture or sequentially with imazethapyr, were evaluated on imidazolinone-susceptible and -resistant barnyardgrass byotipes. In addition, the degradation of imazethapyr was analyzed in plants treated with imazethapyr applied alone or two hours after malathion or NA. The spraying of malathion and PBO reduced the resistance factor (RF) from 15.92 to 3.44 and 4.99, respectively, in the resistant population PALMS01. Conversely, the cytP450 inducer NA increased the RF from 4.45 to 8.32. Malathion increased imazethapyr concentrations in resistant barnyardgrass in comparison with plants sprayed with the herbicide alone, indicating the inhibition of imazethapyr degradation. The simultaneous spraying of malathion and imazethapyr was less efficient than the previous application of this cytP450 inhibitor. These results indicate that degradation enhancement caused by cytP450 enzymes is involved in the resistance mechanism of barnyardgrass to imazethapyr, and appropriate measures should be taken to manage these populations. |
| first_indexed | 2024-12-19T21:00:35Z |
| format | Article |
| id | doaj.art-5a02f4eedb6d452b82c6a65446f68a69 |
| institution | Directory Open Access Journal |
| issn | 0100-8358 |
| language | English |
| last_indexed | 2024-12-19T21:00:35Z |
| publishDate | 2018-10-01 |
| publisher | Sociedade Brasileira da Ciência das Plantas Daninhas |
| record_format | Article |
| series | Planta Daninha |
| spelling | doaj.art-5a02f4eedb6d452b82c6a65446f68a692022-12-21T20:05:49ZengSociedade Brasileira da Ciência das Plantas DaninhasPlanta Daninha0100-83582018-10-013610.1590/s0100-83582018360100119Degradation Enhancement as the Mechanism of Resistance to Imazethapyr in BarnyardgrassG. DALAZENA. PISONIR.S. RAFAELIA. MEROTTO JR.ABSTRACT: Non-target site (NTS) herbicide resistance by degradation enhancement is an increasing problem in several weeds around the world. In this study, the occurrence of degradation enhancement by cytochrome P450 monooxygenases (cytP450) was evaluated as the mechanism of resistance to imazethapyr in barnyardgrass. The cytP450 inhibitors malathion and piperonyl butoxide (PBO) and the inducer naphthalic anhydride (NA), applied in mixture or sequentially with imazethapyr, were evaluated on imidazolinone-susceptible and -resistant barnyardgrass byotipes. In addition, the degradation of imazethapyr was analyzed in plants treated with imazethapyr applied alone or two hours after malathion or NA. The spraying of malathion and PBO reduced the resistance factor (RF) from 15.92 to 3.44 and 4.99, respectively, in the resistant population PALMS01. Conversely, the cytP450 inducer NA increased the RF from 4.45 to 8.32. Malathion increased imazethapyr concentrations in resistant barnyardgrass in comparison with plants sprayed with the herbicide alone, indicating the inhibition of imazethapyr degradation. The simultaneous spraying of malathion and imazethapyr was less efficient than the previous application of this cytP450 inhibitor. These results indicate that degradation enhancement caused by cytP450 enzymes is involved in the resistance mechanism of barnyardgrass to imazethapyr, and appropriate measures should be taken to manage these populations.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582018000100317&tlng=enEchinochloa crus-galliherbicide detoxificationweed resistanceimidazolinonescytP450 |
| spellingShingle | G. DALAZEN A. PISONI R.S. RAFAELI A. MEROTTO JR. Degradation Enhancement as the Mechanism of Resistance to Imazethapyr in Barnyardgrass Planta Daninha Echinochloa crus-galli herbicide detoxification weed resistance imidazolinones cytP450 |
| title | Degradation Enhancement as the Mechanism of Resistance to Imazethapyr in Barnyardgrass |
| title_full | Degradation Enhancement as the Mechanism of Resistance to Imazethapyr in Barnyardgrass |
| title_fullStr | Degradation Enhancement as the Mechanism of Resistance to Imazethapyr in Barnyardgrass |
| title_full_unstemmed | Degradation Enhancement as the Mechanism of Resistance to Imazethapyr in Barnyardgrass |
| title_short | Degradation Enhancement as the Mechanism of Resistance to Imazethapyr in Barnyardgrass |
| title_sort | degradation enhancement as the mechanism of resistance to imazethapyr in barnyardgrass |
| topic | Echinochloa crus-galli herbicide detoxification weed resistance imidazolinones cytP450 |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582018000100317&tlng=en |
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