Quantitative Examination of Pre-Extraction Treatment on the Determination of Lignin Content in Leaves
It has been suggested that the Klason method overestimates the lignin content of non-wood tissues of plants. To evaluate the effect of pre-extraction treatments on lignin determination in leaves, nine kinds of pre-extraction treatment were applied to ginkgo leaves and zelkova leaves. The apparent li...
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| Format: | Article |
| Language: | English |
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North Carolina State University
2015-02-01
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| Series: | BioResources |
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| Online Access: | http://ojs.cnr.ncsu.edu/index.php/BioRes/article/view/BioRes_10_2_2328_Toda_Quantitative_Preextraction_Lignin_Leaf |
| _version_ | 1828466224297672704 |
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| author | Morikazu Toda Takuya Akiyama Tomoya Yokoyama Yuji Matsumoto |
| author_facet | Morikazu Toda Takuya Akiyama Tomoya Yokoyama Yuji Matsumoto |
| author_sort | Morikazu Toda |
| collection | DOAJ |
| description | It has been suggested that the Klason method overestimates the lignin content of non-wood tissues of plants. To evaluate the effect of pre-extraction treatments on lignin determination in leaves, nine kinds of pre-extraction treatment were applied to ginkgo leaves and zelkova leaves. The apparent lignin contents (lignin determination by the Klason method) of ginkgo and zelkova leaves without pre-extraction treatments were 30.7% and 42.6%, respectively. After the various pre-extraction treatments, the apparent lignin contents were still high. On the other hand, the yield of nitrobenzene oxidation products (NPs) per apparent lignin content was maintained at a very low level before and after pre-extraction treatments (maximum value was 6% for ginkgo leaf and 11% for zelkova leaf after extraction treatment) compared with the value from wood (25 to 60%). These results suggested that the Klason method overestimates the lignin content of leaves even after the pre-extraction treatments examined in this study. In addition, a considerable part of the sample from which NPs or neutral sugars originate was lost during these pre-extraction stages. These results implied that some parts of the cell wall components were also removed by these pre-extraction treatments. |
| first_indexed | 2024-12-11T03:47:25Z |
| format | Article |
| id | doaj.art-5a07a6ad17344a43aadde855fffa954f |
| institution | Directory Open Access Journal |
| issn | 1930-2126 1930-2126 |
| language | English |
| last_indexed | 2024-12-11T03:47:25Z |
| publishDate | 2015-02-01 |
| publisher | North Carolina State University |
| record_format | Article |
| series | BioResources |
| spelling | doaj.art-5a07a6ad17344a43aadde855fffa954f2022-12-22T01:21:59ZengNorth Carolina State UniversityBioResources1930-21261930-21262015-02-011022328233710.15376/biores.10.2.2328-2337Quantitative Examination of Pre-Extraction Treatment on the Determination of Lignin Content in LeavesMorikazu Toda0Takuya Akiyama1Tomoya Yokoyama2Yuji Matsumoto3University of Tokyo; JapanUniversity of Tokyo; JapanUniversity of Tokyo; JapanUniversity of Tokyo; JapanIt has been suggested that the Klason method overestimates the lignin content of non-wood tissues of plants. To evaluate the effect of pre-extraction treatments on lignin determination in leaves, nine kinds of pre-extraction treatment were applied to ginkgo leaves and zelkova leaves. The apparent lignin contents (lignin determination by the Klason method) of ginkgo and zelkova leaves without pre-extraction treatments were 30.7% and 42.6%, respectively. After the various pre-extraction treatments, the apparent lignin contents were still high. On the other hand, the yield of nitrobenzene oxidation products (NPs) per apparent lignin content was maintained at a very low level before and after pre-extraction treatments (maximum value was 6% for ginkgo leaf and 11% for zelkova leaf after extraction treatment) compared with the value from wood (25 to 60%). These results suggested that the Klason method overestimates the lignin content of leaves even after the pre-extraction treatments examined in this study. In addition, a considerable part of the sample from which NPs or neutral sugars originate was lost during these pre-extraction stages. These results implied that some parts of the cell wall components were also removed by these pre-extraction treatments.http://ojs.cnr.ncsu.edu/index.php/BioRes/article/view/BioRes_10_2_2328_Toda_Quantitative_Preextraction_Lignin_LeafLigninLeafExtractionKlason ligninBiomass |
| spellingShingle | Morikazu Toda Takuya Akiyama Tomoya Yokoyama Yuji Matsumoto Quantitative Examination of Pre-Extraction Treatment on the Determination of Lignin Content in Leaves BioResources Lignin Leaf Extraction Klason lignin Biomass |
| title | Quantitative Examination of Pre-Extraction Treatment on the Determination of Lignin Content in Leaves |
| title_full | Quantitative Examination of Pre-Extraction Treatment on the Determination of Lignin Content in Leaves |
| title_fullStr | Quantitative Examination of Pre-Extraction Treatment on the Determination of Lignin Content in Leaves |
| title_full_unstemmed | Quantitative Examination of Pre-Extraction Treatment on the Determination of Lignin Content in Leaves |
| title_short | Quantitative Examination of Pre-Extraction Treatment on the Determination of Lignin Content in Leaves |
| title_sort | quantitative examination of pre extraction treatment on the determination of lignin content in leaves |
| topic | Lignin Leaf Extraction Klason lignin Biomass |
| url | http://ojs.cnr.ncsu.edu/index.php/BioRes/article/view/BioRes_10_2_2328_Toda_Quantitative_Preextraction_Lignin_Leaf |
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