Gut microbiota affects obesity susceptibility in mice through gut metabolites
IntroductionIt is well-known that different populations and animals, even experimental animals with the same rearing conditions, differ in their susceptibility to obesity. The disparity in gut microbiota could potentially account for the variation in susceptibility to obesity. However, the precise i...
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| Format: | Article |
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Frontiers Media S.A.
2024-02-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2024.1343511/full |
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| author | Yuhang Wen Yuhang Wen Yadan Luo Yadan Luo Hao Qiu Hao Qiu Baoting Chen Baoting Chen Jingrong Huang Jingrong Huang Shuya Lv Shuya Lv Yan Wang Yan Wang Jiabi Li Jiabi Li Lingling Tao Lingling Tao Bailin Yang Bailin Yang Ke Li Ke Li Lvqin He Lvqin He Manli He Manli He Qian Yang Qian Yang Zehui Yu Zehui Yu Wudian Xiao Wudian Xiao Mingde Zhao Mingde Zhao Xiaoxia Zou Ruilin Lu Congwei Gu Congwei Gu |
| author_facet | Yuhang Wen Yuhang Wen Yadan Luo Yadan Luo Hao Qiu Hao Qiu Baoting Chen Baoting Chen Jingrong Huang Jingrong Huang Shuya Lv Shuya Lv Yan Wang Yan Wang Jiabi Li Jiabi Li Lingling Tao Lingling Tao Bailin Yang Bailin Yang Ke Li Ke Li Lvqin He Lvqin He Manli He Manli He Qian Yang Qian Yang Zehui Yu Zehui Yu Wudian Xiao Wudian Xiao Mingde Zhao Mingde Zhao Xiaoxia Zou Ruilin Lu Congwei Gu Congwei Gu |
| author_sort | Yuhang Wen |
| collection | DOAJ |
| description | IntroductionIt is well-known that different populations and animals, even experimental animals with the same rearing conditions, differ in their susceptibility to obesity. The disparity in gut microbiota could potentially account for the variation in susceptibility to obesity. However, the precise impact of gut microbiota on gut metabolites and its subsequent influence on susceptibility to obesity remains uncertain.MethodsIn this study, we established obesity-prone (OP) and obesity-resistant (OR) mouse models by High Fat Diet (HFD). Fecal contents of cecum were examined using 16S rDNA sequencing and untargeted metabolomics. Correlation analysis and MIMOSA2 analysis were used to explore the association between gut microbiota and intestinal metabolites.ResultsAfter a HFD, gut microbiota and gut metabolic profiles were significantly different between OP and OR mice. Gut microbiota after a HFD may lead to changes in eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA), a variety of branched fatty acid esters of hydroxy fatty acids (FAHFAs) and a variety of phospholipids to promote obesity. The bacteria g_Akkermansia (Greengene ID: 175696) may contribute to the difference in obesity susceptibility through the synthesis of glycerophosphoryl diester phosphodiesterase (glpQ) to promote choline production and the synthesis of valyl-tRNA synthetase (VARS) which promotes L-Valine degradation. In addition, gut microbiota may affect obesity and obesity susceptibility through histidine metabolism, linoleic acid metabolism and protein digestion and absorption pathways. |
| first_indexed | 2024-03-07T23:09:55Z |
| format | Article |
| id | doaj.art-5a08e9e081174a2382c52930074cbfb8 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-03-07T23:09:55Z |
| publishDate | 2024-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-5a08e9e081174a2382c52930074cbfb82024-02-21T14:18:23ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2024-02-011510.3389/fmicb.2024.13435111343511Gut microbiota affects obesity susceptibility in mice through gut metabolitesYuhang Wen0Yuhang Wen1Yadan Luo2Yadan Luo3Hao Qiu4Hao Qiu5Baoting Chen6Baoting Chen7Jingrong Huang8Jingrong Huang9Shuya Lv10Shuya Lv11Yan Wang12Yan Wang13Jiabi Li14Jiabi Li15Lingling Tao16Lingling Tao17Bailin Yang18Bailin Yang19Ke Li20Ke Li21Lvqin He22Lvqin He23Manli He24Manli He25Qian Yang26Qian Yang27Zehui Yu28Zehui Yu29Wudian Xiao30Wudian Xiao31Mingde Zhao32Mingde Zhao33Xiaoxia Zou34Ruilin Lu35Congwei Gu36Congwei Gu37Laboratory Animal Centre, Southwest Medical University, Luzhou, ChinaModel Animal and Human Disease Research of Luzhou Key Laboratory, Luzhou, ChinaLaboratory Animal Centre, Southwest Medical University, Luzhou, ChinaModel Animal and Human Disease Research of Luzhou Key Laboratory, Luzhou, ChinaLaboratory Animal Centre, Southwest Medical University, Luzhou, ChinaModel Animal and Human Disease Research of Luzhou Key Laboratory, Luzhou, ChinaLaboratory Animal Centre, Southwest Medical University, Luzhou, ChinaModel Animal and Human Disease Research of Luzhou Key Laboratory, Luzhou, ChinaLaboratory Animal Centre, Southwest Medical University, Luzhou, ChinaModel Animal and Human Disease Research of Luzhou Key Laboratory, Luzhou, ChinaLaboratory Animal Centre, Southwest Medical University, Luzhou, ChinaModel Animal and Human Disease Research of Luzhou Key Laboratory, Luzhou, ChinaLaboratory Animal Centre, Southwest Medical University, Luzhou, ChinaModel Animal and Human Disease Research of Luzhou Key Laboratory, Luzhou, ChinaLaboratory Animal Centre, Southwest Medical University, Luzhou, ChinaModel Animal and Human Disease Research of Luzhou Key Laboratory, Luzhou, ChinaLaboratory Animal Centre, Southwest Medical University, Luzhou, ChinaModel Animal and Human Disease Research of Luzhou Key Laboratory, Luzhou, ChinaLaboratory Animal Centre, Southwest Medical University, Luzhou, ChinaModel Animal and Human Disease Research of Luzhou Key Laboratory, Luzhou, ChinaLaboratory Animal Centre, Southwest Medical University, Luzhou, ChinaModel Animal and Human Disease Research of Luzhou Key Laboratory, Luzhou, ChinaLaboratory Animal Centre, Southwest Medical University, Luzhou, ChinaModel Animal and Human Disease Research of Luzhou Key Laboratory, Luzhou, ChinaLaboratory Animal Centre, Southwest Medical University, Luzhou, ChinaModel Animal and Human Disease Research of Luzhou Key Laboratory, Luzhou, ChinaLaboratory Animal Centre, Southwest Medical University, Luzhou, ChinaModel Animal and Human Disease Research of Luzhou Key Laboratory, Luzhou, ChinaLaboratory Animal Centre, Southwest Medical University, Luzhou, ChinaModel Animal and Human Disease Research of Luzhou Key Laboratory, Luzhou, ChinaLaboratory Animal Centre, Southwest Medical University, Luzhou, ChinaModel Animal and Human Disease Research of Luzhou Key Laboratory, Luzhou, ChinaLaboratory Animal Centre, Southwest Medical University, Luzhou, ChinaModel Animal and Human Disease Research of Luzhou Key Laboratory, Luzhou, ChinaSuining First People's Hospital, Suining, ChinaSuining First People's Hospital, Suining, ChinaLaboratory Animal Centre, Southwest Medical University, Luzhou, ChinaModel Animal and Human Disease Research of Luzhou Key Laboratory, Luzhou, ChinaIntroductionIt is well-known that different populations and animals, even experimental animals with the same rearing conditions, differ in their susceptibility to obesity. The disparity in gut microbiota could potentially account for the variation in susceptibility to obesity. However, the precise impact of gut microbiota on gut metabolites and its subsequent influence on susceptibility to obesity remains uncertain.MethodsIn this study, we established obesity-prone (OP) and obesity-resistant (OR) mouse models by High Fat Diet (HFD). Fecal contents of cecum were examined using 16S rDNA sequencing and untargeted metabolomics. Correlation analysis and MIMOSA2 analysis were used to explore the association between gut microbiota and intestinal metabolites.ResultsAfter a HFD, gut microbiota and gut metabolic profiles were significantly different between OP and OR mice. Gut microbiota after a HFD may lead to changes in eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA), a variety of branched fatty acid esters of hydroxy fatty acids (FAHFAs) and a variety of phospholipids to promote obesity. The bacteria g_Akkermansia (Greengene ID: 175696) may contribute to the difference in obesity susceptibility through the synthesis of glycerophosphoryl diester phosphodiesterase (glpQ) to promote choline production and the synthesis of valyl-tRNA synthetase (VARS) which promotes L-Valine degradation. In addition, gut microbiota may affect obesity and obesity susceptibility through histidine metabolism, linoleic acid metabolism and protein digestion and absorption pathways.https://www.frontiersin.org/articles/10.3389/fmicb.2024.1343511/fullsusceptibility to obesitygut microbiotauntargeted metabolomicshigh-fat dietobesity-proneobesity-resistant |
| spellingShingle | Yuhang Wen Yuhang Wen Yadan Luo Yadan Luo Hao Qiu Hao Qiu Baoting Chen Baoting Chen Jingrong Huang Jingrong Huang Shuya Lv Shuya Lv Yan Wang Yan Wang Jiabi Li Jiabi Li Lingling Tao Lingling Tao Bailin Yang Bailin Yang Ke Li Ke Li Lvqin He Lvqin He Manli He Manli He Qian Yang Qian Yang Zehui Yu Zehui Yu Wudian Xiao Wudian Xiao Mingde Zhao Mingde Zhao Xiaoxia Zou Ruilin Lu Congwei Gu Congwei Gu Gut microbiota affects obesity susceptibility in mice through gut metabolites Frontiers in Microbiology susceptibility to obesity gut microbiota untargeted metabolomics high-fat diet obesity-prone obesity-resistant |
| title | Gut microbiota affects obesity susceptibility in mice through gut metabolites |
| title_full | Gut microbiota affects obesity susceptibility in mice through gut metabolites |
| title_fullStr | Gut microbiota affects obesity susceptibility in mice through gut metabolites |
| title_full_unstemmed | Gut microbiota affects obesity susceptibility in mice through gut metabolites |
| title_short | Gut microbiota affects obesity susceptibility in mice through gut metabolites |
| title_sort | gut microbiota affects obesity susceptibility in mice through gut metabolites |
| topic | susceptibility to obesity gut microbiota untargeted metabolomics high-fat diet obesity-prone obesity-resistant |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2024.1343511/full |
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