Detection of group A beta-hemolytic Streptococcus employing three different detection methods: culture, rapid antigen detecting test, and molecular assay
In order to study the prevalence of Group A beta-hemolytic Streptococcus (GABHS) pharyngotonsillitis in our pediatric population and to compare different sampling methods of GABHS detection, oropharyngeal swabs from 50 children with acute pharyngotonsillitis, between 1 and 12 years old, were used si...
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| Format: | Article |
| Language: | English |
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Elsevier
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| Series: | Brazilian Journal of Infectious Diseases |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702003000500003&lng=en&tlng=en |
| _version_ | 1818550132028735488 |
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| author | Odimara Santos Luc Louis Maurice Weckx Antonio Carlos Campos Pignatari Shirley Shizue Nagata Pignatari |
| author_facet | Odimara Santos Luc Louis Maurice Weckx Antonio Carlos Campos Pignatari Shirley Shizue Nagata Pignatari |
| author_sort | Odimara Santos |
| collection | DOAJ |
| description | In order to study the prevalence of Group A beta-hemolytic Streptococcus (GABHS) pharyngotonsillitis in our pediatric population and to compare different sampling methods of GABHS detection, oropharyngeal swabs from 50 children with acute pharyngotonsillitis, between 1 and 12 years old, were used simultaneously for culture, molecular assay and rapid GABHS antigen detection tests. All children were clinically examined at the Division of Pediatric Otorhinolaryngology of the Federal University of São Paulo. Diagnostic criteria were based on signs and symptoms, including sore throat, fever and oropharyngeal purulent secretion. Children that had been treated with antibiotics were excluded. Overall, combining the three methods, the prevalence of GABHS was 34%. GABHS was diagnosed in 30% of the bacterial cultures, in 25% of the samples tested with the molecular nucleic acid hybridization method and in 26% of the cases tested with the rapid antigen detection test. There was no significant difference between these three methods. |
| first_indexed | 2024-12-12T08:42:22Z |
| format | Article |
| id | doaj.art-5a0c80c967fd451b8daf21cdef6ad04f |
| institution | Directory Open Access Journal |
| issn | 1678-4391 |
| language | English |
| last_indexed | 2024-12-12T08:42:22Z |
| publisher | Elsevier |
| record_format | Article |
| series | Brazilian Journal of Infectious Diseases |
| spelling | doaj.art-5a0c80c967fd451b8daf21cdef6ad04f2022-12-22T00:30:44ZengElsevierBrazilian Journal of Infectious Diseases1678-43917529730010.1590/S1413-86702003000500003S1413-86702003000500003Detection of group A beta-hemolytic Streptococcus employing three different detection methods: culture, rapid antigen detecting test, and molecular assayOdimara Santos0Luc Louis Maurice Weckx1Antonio Carlos Campos PignatariShirley Shizue Nagata Pignatari2Universidade Federal de São PauloUniversidade Federal de São PauloUniversidade Federal de São PauloIn order to study the prevalence of Group A beta-hemolytic Streptococcus (GABHS) pharyngotonsillitis in our pediatric population and to compare different sampling methods of GABHS detection, oropharyngeal swabs from 50 children with acute pharyngotonsillitis, between 1 and 12 years old, were used simultaneously for culture, molecular assay and rapid GABHS antigen detection tests. All children were clinically examined at the Division of Pediatric Otorhinolaryngology of the Federal University of São Paulo. Diagnostic criteria were based on signs and symptoms, including sore throat, fever and oropharyngeal purulent secretion. Children that had been treated with antibiotics were excluded. Overall, combining the three methods, the prevalence of GABHS was 34%. GABHS was diagnosed in 30% of the bacterial cultures, in 25% of the samples tested with the molecular nucleic acid hybridization method and in 26% of the cases tested with the rapid antigen detection test. There was no significant difference between these three methods.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702003000500003&lng=en&tlng=enAcute pharyngotonsillitisGABHSlaboratory detection |
| spellingShingle | Odimara Santos Luc Louis Maurice Weckx Antonio Carlos Campos Pignatari Shirley Shizue Nagata Pignatari Detection of group A beta-hemolytic Streptococcus employing three different detection methods: culture, rapid antigen detecting test, and molecular assay Brazilian Journal of Infectious Diseases Acute pharyngotonsillitis GABHS laboratory detection |
| title | Detection of group A beta-hemolytic Streptococcus employing three different detection methods: culture, rapid antigen detecting test, and molecular assay |
| title_full | Detection of group A beta-hemolytic Streptococcus employing three different detection methods: culture, rapid antigen detecting test, and molecular assay |
| title_fullStr | Detection of group A beta-hemolytic Streptococcus employing three different detection methods: culture, rapid antigen detecting test, and molecular assay |
| title_full_unstemmed | Detection of group A beta-hemolytic Streptococcus employing three different detection methods: culture, rapid antigen detecting test, and molecular assay |
| title_short | Detection of group A beta-hemolytic Streptococcus employing three different detection methods: culture, rapid antigen detecting test, and molecular assay |
| title_sort | detection of group a beta hemolytic streptococcus employing three different detection methods culture rapid antigen detecting test and molecular assay |
| topic | Acute pharyngotonsillitis GABHS laboratory detection |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702003000500003&lng=en&tlng=en |
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