Evaluation of the Modified Flourescence Staining Differential on Pseudomonas spp. Isolated From Soil
The modified fluorescence staining differential was evaluated using Pseudomonas spp. isolated from cultivated agricultural soils in the State of Sucre, in order to observe microscopic events related to the cellular cycle. Each species of biochemically identified Pseudomonas was inoculated into a bro...
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| Format: | Article |
| Language: | English |
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Universidad del Zulia,Facultad de Medicina,Departamento de Enfermedades Infecciosas y Tropicales
2008-06-01
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| Series: | Kasmera |
| Subjects: | |
| Online Access: | https://produccioncientificaluz.org/index.php/kasmera/article/view/4819 |
| _version_ | 1797710159744598016 |
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| author | Luzmila Albarado Evelin Flores |
| author_facet | Luzmila Albarado Evelin Flores |
| author_sort | Luzmila Albarado |
| collection | DOAJ |
| description | The modified fluorescence staining differential was evaluated using Pseudomonas spp. isolated from cultivated agricultural soils in the State of Sucre, in order to observe microscopic events related to the cellular cycle. Each species of biochemically identified Pseudomonas was inoculated into a broth and incubated at room temperature, aerobiosis, for 15, 20, 30 and 45 minutes and 1, 24, 48 and 72 hours; then, slides were made and stained. For the 24 identified strains of Pseudomonas, P. mendocina (41.67%), P. aeruginosa (37.50%) and P. putida (20.83%), staining variations such as green, yellow and orange, fluorescent and low fluorescence were observed for the different incubation times. The stain uses acridine orange that interacts with DNA by intercalation, causing green fluorescence; it interacts withRNAby electrostatic attraction causing orange fluorescence; the alcohol-acetone decolorant removes the acridine orange not united with the genetic material and sodium fluorescein produces a yellow color in bacteria that retain a sufficient amount of acridine orange. Cytoplasmatic staining variations in Pseudomonas spp., are associated with the amount of RNA and DNA present in the cells according to the phase of their cellular cycle. |
| first_indexed | 2024-03-12T06:48:12Z |
| format | Article |
| id | doaj.art-5a0fa034325543a78589a4520ccb934b |
| institution | Directory Open Access Journal |
| issn | 0075-5222 2477-9628 |
| language | English |
| last_indexed | 2024-03-12T06:48:12Z |
| publishDate | 2008-06-01 |
| publisher | Universidad del Zulia,Facultad de Medicina,Departamento de Enfermedades Infecciosas y Tropicales |
| record_format | Article |
| series | Kasmera |
| spelling | doaj.art-5a0fa034325543a78589a4520ccb934b2023-09-03T00:27:53ZengUniversidad del Zulia,Facultad de Medicina,Departamento de Enfermedades Infecciosas y TropicalesKasmera0075-52222477-96282008-06-01361172724319Evaluation of the Modified Flourescence Staining Differential on Pseudomonas spp. Isolated From SoilLuzmila Albarado0Evelin Flores1Universidad de Oriente, Núcleo de Sucre. Escuela de Ciencias, Departamento de Bioanálisis, Laboratorio de Microbiología General. Cumaná, Venezuela.Universidad de Oriente, Núcleo de Sucre. Escuela de Ciencias, Departamento de Bioanálisis, Laboratorio de Histología. Cumaná, Venezuela.The modified fluorescence staining differential was evaluated using Pseudomonas spp. isolated from cultivated agricultural soils in the State of Sucre, in order to observe microscopic events related to the cellular cycle. Each species of biochemically identified Pseudomonas was inoculated into a broth and incubated at room temperature, aerobiosis, for 15, 20, 30 and 45 minutes and 1, 24, 48 and 72 hours; then, slides were made and stained. For the 24 identified strains of Pseudomonas, P. mendocina (41.67%), P. aeruginosa (37.50%) and P. putida (20.83%), staining variations such as green, yellow and orange, fluorescent and low fluorescence were observed for the different incubation times. The stain uses acridine orange that interacts with DNA by intercalation, causing green fluorescence; it interacts withRNAby electrostatic attraction causing orange fluorescence; the alcohol-acetone decolorant removes the acridine orange not united with the genetic material and sodium fluorescein produces a yellow color in bacteria that retain a sufficient amount of acridine orange. Cytoplasmatic staining variations in Pseudomonas spp., are associated with the amount of RNA and DNA present in the cells according to the phase of their cellular cycle.https://produccioncientificaluz.org/index.php/kasmera/article/view/4819Pseudomonas spp., coloración de fluorescencia modificada, naranja de acridina, fluoresceína, suelos |
| spellingShingle | Luzmila Albarado Evelin Flores Evaluation of the Modified Flourescence Staining Differential on Pseudomonas spp. Isolated From Soil Kasmera Pseudomonas spp., coloración de fluorescencia modificada, naranja de acridina, fluoresceína, suelos |
| title | Evaluation of the Modified Flourescence Staining Differential on Pseudomonas spp. Isolated From Soil |
| title_full | Evaluation of the Modified Flourescence Staining Differential on Pseudomonas spp. Isolated From Soil |
| title_fullStr | Evaluation of the Modified Flourescence Staining Differential on Pseudomonas spp. Isolated From Soil |
| title_full_unstemmed | Evaluation of the Modified Flourescence Staining Differential on Pseudomonas spp. Isolated From Soil |
| title_short | Evaluation of the Modified Flourescence Staining Differential on Pseudomonas spp. Isolated From Soil |
| title_sort | evaluation of the modified flourescence staining differential on pseudomonas spp isolated from soil |
| topic | Pseudomonas spp., coloración de fluorescencia modificada, naranja de acridina, fluoresceína, suelos |
| url | https://produccioncientificaluz.org/index.php/kasmera/article/view/4819 |
| work_keys_str_mv | AT luzmilaalbarado evaluationofthemodifiedflourescencestainingdifferentialonpseudomonassppisolatedfromsoil AT evelinflores evaluationofthemodifiedflourescencestainingdifferentialonpseudomonassppisolatedfromsoil |