Potential Biomarkers and Endometrial Immune Microenvironment in Recurrent Implantation Failure
The molecular mechanisms underlying unexplained recurrent implantation failure (RIF) remain unclear. This study aimed at identifying potential biomarkers, exploring relevant signaling pathways, and analyzing the contribution of immune cell infiltration in RIF. Microarray expression datasets were ext...
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MDPI AG
2023-02-01
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Online Access: | https://www.mdpi.com/2218-273X/13/3/406 |
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author | Fangfang Li Wenxin Gao Yanmei Li Yiqing Wang Lin Liu Xuehong Zhang |
author_facet | Fangfang Li Wenxin Gao Yanmei Li Yiqing Wang Lin Liu Xuehong Zhang |
author_sort | Fangfang Li |
collection | DOAJ |
description | The molecular mechanisms underlying unexplained recurrent implantation failure (RIF) remain unclear. This study aimed at identifying potential biomarkers, exploring relevant signaling pathways, and analyzing the contribution of immune cell infiltration in RIF. Microarray expression datasets were extracted from the Gene Expression Omnibus database to perform bioinformatic analyses. The results showed that ten hub genes may predict RIF with high specificity and sensitivity (area under the curve = 1.000). Protein–protein interaction analysis revealed close interactions between the hub genes and the endometrial receptivity array. The real-time quantitative polymerase chain reaction further validated three potential biomarkers (<i>RAB32, TRIB2,</i> and <i>FAM155B</i>). Functional enrichment analyses indicated that immune pathways were significantly downregulated and lipid metabolism pathways were significantly upregulated in RIF compared with the controls. Significant negative correlations were observed between fatty acid biosynthesis and the immune pathways. Immune cell infiltration, including those in CD56dim natural killer, dendritic, Th1, Th2, and regulatory T cells, as well as macrophages, was significantly reduced in RIF compared with the controls used herein. This study may provide a novel perspective on the diagnosis and treatment of RIF. |
first_indexed | 2024-03-11T06:52:30Z |
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institution | Directory Open Access Journal |
issn | 2218-273X |
language | English |
last_indexed | 2024-03-11T06:52:30Z |
publishDate | 2023-02-01 |
publisher | MDPI AG |
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series | Biomolecules |
spelling | doaj.art-5a0ff45867bc402396c33ab5ae96db682023-11-17T09:50:50ZengMDPI AGBiomolecules2218-273X2023-02-0113340610.3390/biom13030406Potential Biomarkers and Endometrial Immune Microenvironment in Recurrent Implantation FailureFangfang Li0Wenxin Gao1Yanmei Li2Yiqing Wang3Lin Liu4Xuehong Zhang5The First School of Clinical Medicine, Lanzhou University, Lanzhou 730000, ChinaThe First School of Clinical Medicine, Lanzhou University, Lanzhou 730000, ChinaThe First School of Clinical Medicine, Lanzhou University, Lanzhou 730000, ChinaThe First School of Clinical Medicine, Lanzhou University, Lanzhou 730000, ChinaThe First School of Clinical Medicine, Lanzhou University, Lanzhou 730000, ChinaThe First School of Clinical Medicine, Lanzhou University, Lanzhou 730000, ChinaThe molecular mechanisms underlying unexplained recurrent implantation failure (RIF) remain unclear. This study aimed at identifying potential biomarkers, exploring relevant signaling pathways, and analyzing the contribution of immune cell infiltration in RIF. Microarray expression datasets were extracted from the Gene Expression Omnibus database to perform bioinformatic analyses. The results showed that ten hub genes may predict RIF with high specificity and sensitivity (area under the curve = 1.000). Protein–protein interaction analysis revealed close interactions between the hub genes and the endometrial receptivity array. The real-time quantitative polymerase chain reaction further validated three potential biomarkers (<i>RAB32, TRIB2,</i> and <i>FAM155B</i>). Functional enrichment analyses indicated that immune pathways were significantly downregulated and lipid metabolism pathways were significantly upregulated in RIF compared with the controls. Significant negative correlations were observed between fatty acid biosynthesis and the immune pathways. Immune cell infiltration, including those in CD56dim natural killer, dendritic, Th1, Th2, and regulatory T cells, as well as macrophages, was significantly reduced in RIF compared with the controls used herein. This study may provide a novel perspective on the diagnosis and treatment of RIF.https://www.mdpi.com/2218-273X/13/3/406recurrent implantation failurebiomarkerimmune cell infiltrationimmune pathwaylipid metabolism |
spellingShingle | Fangfang Li Wenxin Gao Yanmei Li Yiqing Wang Lin Liu Xuehong Zhang Potential Biomarkers and Endometrial Immune Microenvironment in Recurrent Implantation Failure Biomolecules recurrent implantation failure biomarker immune cell infiltration immune pathway lipid metabolism |
title | Potential Biomarkers and Endometrial Immune Microenvironment in Recurrent Implantation Failure |
title_full | Potential Biomarkers and Endometrial Immune Microenvironment in Recurrent Implantation Failure |
title_fullStr | Potential Biomarkers and Endometrial Immune Microenvironment in Recurrent Implantation Failure |
title_full_unstemmed | Potential Biomarkers and Endometrial Immune Microenvironment in Recurrent Implantation Failure |
title_short | Potential Biomarkers and Endometrial Immune Microenvironment in Recurrent Implantation Failure |
title_sort | potential biomarkers and endometrial immune microenvironment in recurrent implantation failure |
topic | recurrent implantation failure biomarker immune cell infiltration immune pathway lipid metabolism |
url | https://www.mdpi.com/2218-273X/13/3/406 |
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