Phylogenetic Characteristics of Canine Parvovirus Type 2c Variant Endemic in Shanghai, China
Canine parvovirus type 2 (CPV-2) has spread and mutated globally over the past 40 years. In the present study, 206 samples from dogs suspected of CPV-2 infection were collected from five veterinary clinics in Shanghai city, China. The average positive rate for CPV-2 was detected to be 40.78% using t...
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MDPI AG
2021-11-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/13/11/2257 |
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| author | Chengqian Liu Jun Gao Hong Li Fengping Sun Hongyu Liang Huili Liu Jianzhong Yi |
| author_facet | Chengqian Liu Jun Gao Hong Li Fengping Sun Hongyu Liang Huili Liu Jianzhong Yi |
| author_sort | Chengqian Liu |
| collection | DOAJ |
| description | Canine parvovirus type 2 (CPV-2) has spread and mutated globally over the past 40 years. In the present study, 206 samples from dogs suspected of CPV-2 infection were collected from five veterinary clinics in Shanghai city, China. The average positive rate for CPV-2 was detected to be 40.78% using the PCR method. Using an F81 cell (feline kidney cell) culture, the isolates of three CPV-2c strains were obtained. The near full-length genome sequences of the isolates were determined and submitted to GenBank: CPV-SH2001 (MW650830), CPV-SH2002 (MW811188), and CPV-SH2003 (MW811189). By comparing the amino acid sequences of 12 CPV strains with those of 48 related strains retrieved from GenBank, all of the CPV strains from Shanghai were typed as belonging to a relatively new CPV-2c variant spreading in Asia, with typical amino acid residues (5Gly, 267Tyr, 324Ile, and 370Arg) in the VP2 protein. The divergence time of this new CPV-2c clade was estimated by the phylogenetic tree using the maximum likelihood and RelTime with Dated Tips (RTDT) approaches. Our results indicate that the 426 and 324 VP2 amino acid residues are under strong selection pressure with a posterior probability of 0.966 and 0.943, respectively. Therefore, this study provides insight into the phylogenetic characteristics of the current CPV-2c variant in Shanghai city, China. |
| first_indexed | 2024-03-10T04:59:05Z |
| format | Article |
| id | doaj.art-5a102cb9f4c44114a96719c568863a36 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-10T04:59:05Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-5a102cb9f4c44114a96719c568863a362023-11-23T01:57:41ZengMDPI AGViruses1999-49152021-11-011311225710.3390/v13112257Phylogenetic Characteristics of Canine Parvovirus Type 2c Variant Endemic in Shanghai, ChinaChengqian Liu0Jun Gao1Hong Li2Fengping Sun3Hongyu Liang4Huili Liu5Jianzhong Yi6Institute of Animal Husbandry and Veterinary Science, Shanghai Academy of Agricultural Sciences, Shanghai 201106, ChinaInstitute of Animal Husbandry and Veterinary Science, Shanghai Academy of Agricultural Sciences, Shanghai 201106, ChinaInstitute of Animal Husbandry and Veterinary Science, Shanghai Academy of Agricultural Sciences, Shanghai 201106, ChinaInstitute of Animal Husbandry and Veterinary Science, Shanghai Academy of Agricultural Sciences, Shanghai 201106, ChinaNational Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, ChinaInstitute of Animal Husbandry and Veterinary Science, Shanghai Academy of Agricultural Sciences, Shanghai 201106, ChinaInstitute of Animal Husbandry and Veterinary Science, Shanghai Academy of Agricultural Sciences, Shanghai 201106, ChinaCanine parvovirus type 2 (CPV-2) has spread and mutated globally over the past 40 years. In the present study, 206 samples from dogs suspected of CPV-2 infection were collected from five veterinary clinics in Shanghai city, China. The average positive rate for CPV-2 was detected to be 40.78% using the PCR method. Using an F81 cell (feline kidney cell) culture, the isolates of three CPV-2c strains were obtained. The near full-length genome sequences of the isolates were determined and submitted to GenBank: CPV-SH2001 (MW650830), CPV-SH2002 (MW811188), and CPV-SH2003 (MW811189). By comparing the amino acid sequences of 12 CPV strains with those of 48 related strains retrieved from GenBank, all of the CPV strains from Shanghai were typed as belonging to a relatively new CPV-2c variant spreading in Asia, with typical amino acid residues (5Gly, 267Tyr, 324Ile, and 370Arg) in the VP2 protein. The divergence time of this new CPV-2c clade was estimated by the phylogenetic tree using the maximum likelihood and RelTime with Dated Tips (RTDT) approaches. Our results indicate that the 426 and 324 VP2 amino acid residues are under strong selection pressure with a posterior probability of 0.966 and 0.943, respectively. Therefore, this study provides insight into the phylogenetic characteristics of the current CPV-2c variant in Shanghai city, China.https://www.mdpi.com/1999-4915/13/11/2257canine parvovirusCPV-2VP2 capsid protein |
| spellingShingle | Chengqian Liu Jun Gao Hong Li Fengping Sun Hongyu Liang Huili Liu Jianzhong Yi Phylogenetic Characteristics of Canine Parvovirus Type 2c Variant Endemic in Shanghai, China Viruses canine parvovirus CPV-2 VP2 capsid protein |
| title | Phylogenetic Characteristics of Canine Parvovirus Type 2c Variant Endemic in Shanghai, China |
| title_full | Phylogenetic Characteristics of Canine Parvovirus Type 2c Variant Endemic in Shanghai, China |
| title_fullStr | Phylogenetic Characteristics of Canine Parvovirus Type 2c Variant Endemic in Shanghai, China |
| title_full_unstemmed | Phylogenetic Characteristics of Canine Parvovirus Type 2c Variant Endemic in Shanghai, China |
| title_short | Phylogenetic Characteristics of Canine Parvovirus Type 2c Variant Endemic in Shanghai, China |
| title_sort | phylogenetic characteristics of canine parvovirus type 2c variant endemic in shanghai china |
| topic | canine parvovirus CPV-2 VP2 capsid protein |
| url | https://www.mdpi.com/1999-4915/13/11/2257 |
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