Cloning and characterization of the Cerasus humilis sucrose phosphate synthase gene (ChSPS1).
Sucrose is crucial to the growth and development of plants, and sucrose phosphate synthase (SPS) plays a key role in sucrose synthesis. To understand the genetic and molecular mechanisms of sucrose synthesis in Cerasus humilis, ChSPS1, a homologue of SPS, was cloned using RT-PCR. Sequence analysis s...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2017-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC5643142?pdf=render |
| _version_ | 1818131907115745280 |
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| author | Juan Wang Junjie Du Xiaopeng Mu Pengfei Wang |
| author_facet | Juan Wang Junjie Du Xiaopeng Mu Pengfei Wang |
| author_sort | Juan Wang |
| collection | DOAJ |
| description | Sucrose is crucial to the growth and development of plants, and sucrose phosphate synthase (SPS) plays a key role in sucrose synthesis. To understand the genetic and molecular mechanisms of sucrose synthesis in Cerasus humilis, ChSPS1, a homologue of SPS, was cloned using RT-PCR. Sequence analysis showed that the open reading frame (ORF) sequence of ChSPS1 is 3174 bp in length, encoding a predicted protein of 1057 amino acids. The predicted protein showed a high degree of sequence identity with SPS homologues from other species. Real-time RT-PCR analysis showed that ChSPS1 mRNA was detected in all tissues and the transcription level was the highest in mature fruit. There is a significant positive correlation between expression of ChSPS1 and sucrose content. Prokaryotic expression of ChSPS1 indicated that ChSPS1 protein was expressed in E. coli and it had the SPS activity. Overexpression of ChSPS1 in tobacco led to upregulation of enzyme activity and increased sucrose contents in transgenic plants. Real-time RT-PCR analysis showed that the expression of ChSPS1 in transgenic tobacco was significantly higher than in wild type plants. These results suggested that ChSPS1 plays an important role in sucrose synthesis in Cerasus humilis. |
| first_indexed | 2024-12-11T08:28:23Z |
| format | Article |
| id | doaj.art-5a1083f834604d98900d10bea79bf79f |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-11T08:28:23Z |
| publishDate | 2017-01-01 |
| publisher | Public Library of Science (PLoS) |
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| series | PLoS ONE |
| spelling | doaj.art-5a1083f834604d98900d10bea79bf79f2022-12-22T01:14:30ZengPublic Library of Science (PLoS)PLoS ONE1932-62032017-01-011210e018665010.1371/journal.pone.0186650Cloning and characterization of the Cerasus humilis sucrose phosphate synthase gene (ChSPS1).Juan WangJunjie DuXiaopeng MuPengfei WangSucrose is crucial to the growth and development of plants, and sucrose phosphate synthase (SPS) plays a key role in sucrose synthesis. To understand the genetic and molecular mechanisms of sucrose synthesis in Cerasus humilis, ChSPS1, a homologue of SPS, was cloned using RT-PCR. Sequence analysis showed that the open reading frame (ORF) sequence of ChSPS1 is 3174 bp in length, encoding a predicted protein of 1057 amino acids. The predicted protein showed a high degree of sequence identity with SPS homologues from other species. Real-time RT-PCR analysis showed that ChSPS1 mRNA was detected in all tissues and the transcription level was the highest in mature fruit. There is a significant positive correlation between expression of ChSPS1 and sucrose content. Prokaryotic expression of ChSPS1 indicated that ChSPS1 protein was expressed in E. coli and it had the SPS activity. Overexpression of ChSPS1 in tobacco led to upregulation of enzyme activity and increased sucrose contents in transgenic plants. Real-time RT-PCR analysis showed that the expression of ChSPS1 in transgenic tobacco was significantly higher than in wild type plants. These results suggested that ChSPS1 plays an important role in sucrose synthesis in Cerasus humilis.http://europepmc.org/articles/PMC5643142?pdf=render |
| spellingShingle | Juan Wang Junjie Du Xiaopeng Mu Pengfei Wang Cloning and characterization of the Cerasus humilis sucrose phosphate synthase gene (ChSPS1). PLoS ONE |
| title | Cloning and characterization of the Cerasus humilis sucrose phosphate synthase gene (ChSPS1). |
| title_full | Cloning and characterization of the Cerasus humilis sucrose phosphate synthase gene (ChSPS1). |
| title_fullStr | Cloning and characterization of the Cerasus humilis sucrose phosphate synthase gene (ChSPS1). |
| title_full_unstemmed | Cloning and characterization of the Cerasus humilis sucrose phosphate synthase gene (ChSPS1). |
| title_short | Cloning and characterization of the Cerasus humilis sucrose phosphate synthase gene (ChSPS1). |
| title_sort | cloning and characterization of the cerasus humilis sucrose phosphate synthase gene chsps1 |
| url | http://europepmc.org/articles/PMC5643142?pdf=render |
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