In-vitro Antioxidant and In-vitro Anti-inflammatory activities of Ethanolic leaves extract of Ormocarpum Cochinchinense
Introduction: Periodontitis, a chronic inflammatory disease with microbial etiology, is mediated by multiple inflammatory processes and oxidative stress is now well recognized as a part of periodontal pathogenesis. A balance between reactive oxygen species and antioxidants is required to maintain pe...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2022-01-01
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| Series: | Journal of Orofacial Sciences |
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| Online Access: | http://www.jofs.in/article.asp?issn=0975-8844;year=2022;volume=14;issue=2;spage=134;epage=140;aulast=Somashekar |
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| author | Gayathri Somashekar Uma Sudhakar Poonudurai Sameul Gnana Prakash Snophia Suresh Seshadri Srividya Sumathi Hanumantha Rao |
| author_facet | Gayathri Somashekar Uma Sudhakar Poonudurai Sameul Gnana Prakash Snophia Suresh Seshadri Srividya Sumathi Hanumantha Rao |
| author_sort | Gayathri Somashekar |
| collection | DOAJ |
| description | Introduction: Periodontitis, a chronic inflammatory disease with microbial etiology, is mediated by multiple inflammatory processes and oxidative stress is now well recognized as a part of periodontal pathogenesis. A balance between reactive oxygen species and antioxidants is required to maintain periodontal health. Medicinal herbs with bioactive phytocompounds have rich source of antioxidants and anti-inflammatory compounds. Ormocarpum cochinchinense is a medicinal herb with antioxidants and anti-inflammatory phytocompounds. The phytocompounds activities of the herb are not much explored. This study is focused on the In-vitro antioxidant and anti-inflammatory activities of the ethanolic extract of leaves of O. cochinchinense. To assess the In-vitro antioxidant and In-vitro anti-inflammatory activities of ethanolic extracts of O. cochinchinense. Materials and Methods: The leaves of O. cochinchinense were collected, air dried in the shade, and then powdered in an electric blender. The preparation of ethanolic extract was carried out. In-vitro antioxidant studies using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and Nitric Oxide (NO) assays along with anti-inflammatory activity by protein denaturation inhibition and membrane stabilization method were studied. Descriptive statistics were used for continuous variables and expressed in mean and standard deviation. One way ANOVA with post-hoc tukey test or Kruskal–Wallis test, Post-hoc Mann–Whitney U test was used according to the normal distribution of the sample. To compare the individual study group against their standard group, independent t test, and Mann–Whitney U test have been used. P < 0.05 was considered significant. Results: O. cochinchinense had significant antioxidant and anti-inflammatory activities. The ethanolic extract showed dose-dependant activity in all analyses performed (P < 0.05). NO inhibition assay showed 95% of antioxidant activity and 80% of anti-inflammatory activity in the Human Red Blood Cell (HRBC) Membrane Stabilization assay. Conclusions: O. cochinchinense could be used as an adjuvant supplement to conventional therapy in the treatment of chronic inflammatory diseases. |
| first_indexed | 2024-04-10T09:54:07Z |
| format | Article |
| id | doaj.art-5a1307f5d6834d578f168f10dae8acf4 |
| institution | Directory Open Access Journal |
| issn | 0975-8844 |
| language | English |
| last_indexed | 2024-04-10T09:54:07Z |
| publishDate | 2022-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Journal of Orofacial Sciences |
| spelling | doaj.art-5a1307f5d6834d578f168f10dae8acf42023-02-16T12:42:10ZengWolters Kluwer Medknow PublicationsJournal of Orofacial Sciences0975-88442022-01-0114213414010.4103/jofs.jofs_253_22In-vitro Antioxidant and In-vitro Anti-inflammatory activities of Ethanolic leaves extract of Ormocarpum CochinchinenseGayathri SomashekarUma SudhakarPoonudurai Sameul Gnana PrakashSnophia SureshSeshadri SrividyaSumathi Hanumantha RaoIntroduction: Periodontitis, a chronic inflammatory disease with microbial etiology, is mediated by multiple inflammatory processes and oxidative stress is now well recognized as a part of periodontal pathogenesis. A balance between reactive oxygen species and antioxidants is required to maintain periodontal health. Medicinal herbs with bioactive phytocompounds have rich source of antioxidants and anti-inflammatory compounds. Ormocarpum cochinchinense is a medicinal herb with antioxidants and anti-inflammatory phytocompounds. The phytocompounds activities of the herb are not much explored. This study is focused on the In-vitro antioxidant and anti-inflammatory activities of the ethanolic extract of leaves of O. cochinchinense. To assess the In-vitro antioxidant and In-vitro anti-inflammatory activities of ethanolic extracts of O. cochinchinense. Materials and Methods: The leaves of O. cochinchinense were collected, air dried in the shade, and then powdered in an electric blender. The preparation of ethanolic extract was carried out. In-vitro antioxidant studies using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and Nitric Oxide (NO) assays along with anti-inflammatory activity by protein denaturation inhibition and membrane stabilization method were studied. Descriptive statistics were used for continuous variables and expressed in mean and standard deviation. One way ANOVA with post-hoc tukey test or Kruskal–Wallis test, Post-hoc Mann–Whitney U test was used according to the normal distribution of the sample. To compare the individual study group against their standard group, independent t test, and Mann–Whitney U test have been used. P < 0.05 was considered significant. Results: O. cochinchinense had significant antioxidant and anti-inflammatory activities. The ethanolic extract showed dose-dependant activity in all analyses performed (P < 0.05). NO inhibition assay showed 95% of antioxidant activity and 80% of anti-inflammatory activity in the Human Red Blood Cell (HRBC) Membrane Stabilization assay. Conclusions: O. cochinchinense could be used as an adjuvant supplement to conventional therapy in the treatment of chronic inflammatory diseases.http://www.jofs.in/article.asp?issn=0975-8844;year=2022;volume=14;issue=2;spage=134;epage=140;aulast=Somashekaranti-inflammatoryantioxidanto. cochinchinenseperiodontitis |
| spellingShingle | Gayathri Somashekar Uma Sudhakar Poonudurai Sameul Gnana Prakash Snophia Suresh Seshadri Srividya Sumathi Hanumantha Rao In-vitro Antioxidant and In-vitro Anti-inflammatory activities of Ethanolic leaves extract of Ormocarpum Cochinchinense Journal of Orofacial Sciences anti-inflammatory antioxidant o. cochinchinense periodontitis |
| title | In-vitro Antioxidant and In-vitro Anti-inflammatory activities of Ethanolic leaves extract of Ormocarpum Cochinchinense |
| title_full | In-vitro Antioxidant and In-vitro Anti-inflammatory activities of Ethanolic leaves extract of Ormocarpum Cochinchinense |
| title_fullStr | In-vitro Antioxidant and In-vitro Anti-inflammatory activities of Ethanolic leaves extract of Ormocarpum Cochinchinense |
| title_full_unstemmed | In-vitro Antioxidant and In-vitro Anti-inflammatory activities of Ethanolic leaves extract of Ormocarpum Cochinchinense |
| title_short | In-vitro Antioxidant and In-vitro Anti-inflammatory activities of Ethanolic leaves extract of Ormocarpum Cochinchinense |
| title_sort | in vitro antioxidant and in vitro anti inflammatory activities of ethanolic leaves extract of ormocarpum cochinchinense |
| topic | anti-inflammatory antioxidant o. cochinchinense periodontitis |
| url | http://www.jofs.in/article.asp?issn=0975-8844;year=2022;volume=14;issue=2;spage=134;epage=140;aulast=Somashekar |
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