Screening for Microbial Metal-Chelating Siderophores for the Removal of Metal Ions from Solutions
To guarantee the supply of critical elements in the future, the development of new technologies is essential. Siderophores have high potential in the recovery and recycling of valuable metals due to their metal-chelating properties. Using the Chrome azurol S assay, 75 bacterial strains were screened...
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| Format: | Article |
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MDPI AG
2021-01-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/9/1/111 |
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| author | Marika Hofmann Thomas Heine Luise Malik Sarah Hofmann Kristin Joffroy Christoph Helmut Rudi Senges Julia Elisabeth Bandow Dirk Tischler |
| author_facet | Marika Hofmann Thomas Heine Luise Malik Sarah Hofmann Kristin Joffroy Christoph Helmut Rudi Senges Julia Elisabeth Bandow Dirk Tischler |
| author_sort | Marika Hofmann |
| collection | DOAJ |
| description | To guarantee the supply of critical elements in the future, the development of new technologies is essential. Siderophores have high potential in the recovery and recycling of valuable metals due to their metal-chelating properties. Using the Chrome azurol S assay, 75 bacterial strains were screened to obtain a high-yield siderophore with the ability to complex valuable critical metal ions. The siderophore production of the four selected strains <i>Nocardioides simplex</i> 3E, <i>Pseudomonas chlororaphis</i> DSM 50083, <i>Variovorax paradoxus</i> EPS, and <i>Rhodococcus erythropolis</i> B7g was optimized, resulting in significantly increased siderophore production of <i>N. simplex</i> and <i>R. erythropolis</i>. Produced siderophore amounts and velocities were highly dependent on the carbon source. The genomes of <i>N. simplex</i> and <i>P. chlororaphis</i> were sequenced. Bioinformatical analyses revealed the occurrence of an achromobactin and a pyoverdine gene cluster in <i>P. chlororaphis</i>, a heterobactin and a requichelin gene cluster in <i>R. erythropolis</i>, and a desferrioxamine gene cluster in <i>N. simplex.</i> Finally, the results of the previous metal-binding screening were validated by a proof-of-concept development for the recovery of metal ions from aqueous solutions utilizing C<sub>18</sub> columns functionalized with siderophores. We demonstrated the recovery of the critical metal ions V(III), Ga(III), and In(III) from mixed metal solutions with immobilized siderophores of <i>N. simplex</i> and <i>R. erythropolis.</i> |
| first_indexed | 2024-03-10T13:27:58Z |
| format | Article |
| id | doaj.art-5a130b01fea04d0c9e19564ebb5e212f |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T13:27:58Z |
| publishDate | 2021-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-5a130b01fea04d0c9e19564ebb5e212f2023-11-21T08:35:23ZengMDPI AGMicroorganisms2076-26072021-01-019111110.3390/microorganisms9010111Screening for Microbial Metal-Chelating Siderophores for the Removal of Metal Ions from SolutionsMarika Hofmann0Thomas Heine1Luise Malik2Sarah Hofmann3Kristin Joffroy4Christoph Helmut Rudi Senges5Julia Elisabeth Bandow6Dirk Tischler7Institute of Biosciences, TU Bergakademie Freiberg, 09599 Freiberg, GermanyInstitute of Biosciences, TU Bergakademie Freiberg, 09599 Freiberg, GermanyInstitute of Biosciences, TU Bergakademie Freiberg, 09599 Freiberg, GermanyInstitute of Biosciences, TU Bergakademie Freiberg, 09599 Freiberg, GermanyInstitute of Biosciences, TU Bergakademie Freiberg, 09599 Freiberg, GermanyApplied Microbiology, Faculty of Biology and Biotechnology, Ruhr-Universität Bochum, 44780 Bochum, GermanyApplied Microbiology, Faculty of Biology and Biotechnology, Ruhr-Universität Bochum, 44780 Bochum, GermanyMicrobial Biotechnology, Faculty of Biology and Biotechnology, Ruhr-Universität Bochum, 44780 Bochum, GermanyTo guarantee the supply of critical elements in the future, the development of new technologies is essential. Siderophores have high potential in the recovery and recycling of valuable metals due to their metal-chelating properties. Using the Chrome azurol S assay, 75 bacterial strains were screened to obtain a high-yield siderophore with the ability to complex valuable critical metal ions. The siderophore production of the four selected strains <i>Nocardioides simplex</i> 3E, <i>Pseudomonas chlororaphis</i> DSM 50083, <i>Variovorax paradoxus</i> EPS, and <i>Rhodococcus erythropolis</i> B7g was optimized, resulting in significantly increased siderophore production of <i>N. simplex</i> and <i>R. erythropolis</i>. Produced siderophore amounts and velocities were highly dependent on the carbon source. The genomes of <i>N. simplex</i> and <i>P. chlororaphis</i> were sequenced. Bioinformatical analyses revealed the occurrence of an achromobactin and a pyoverdine gene cluster in <i>P. chlororaphis</i>, a heterobactin and a requichelin gene cluster in <i>R. erythropolis</i>, and a desferrioxamine gene cluster in <i>N. simplex.</i> Finally, the results of the previous metal-binding screening were validated by a proof-of-concept development for the recovery of metal ions from aqueous solutions utilizing C<sub>18</sub> columns functionalized with siderophores. We demonstrated the recovery of the critical metal ions V(III), Ga(III), and In(III) from mixed metal solutions with immobilized siderophores of <i>N. simplex</i> and <i>R. erythropolis.</i>https://www.mdpi.com/2076-2607/9/1/111metallophorescreeningCAS assayimmobilizationmetal bindingmetal revocery |
| spellingShingle | Marika Hofmann Thomas Heine Luise Malik Sarah Hofmann Kristin Joffroy Christoph Helmut Rudi Senges Julia Elisabeth Bandow Dirk Tischler Screening for Microbial Metal-Chelating Siderophores for the Removal of Metal Ions from Solutions Microorganisms metallophore screening CAS assay immobilization metal binding metal revocery |
| title | Screening for Microbial Metal-Chelating Siderophores for the Removal of Metal Ions from Solutions |
| title_full | Screening for Microbial Metal-Chelating Siderophores for the Removal of Metal Ions from Solutions |
| title_fullStr | Screening for Microbial Metal-Chelating Siderophores for the Removal of Metal Ions from Solutions |
| title_full_unstemmed | Screening for Microbial Metal-Chelating Siderophores for the Removal of Metal Ions from Solutions |
| title_short | Screening for Microbial Metal-Chelating Siderophores for the Removal of Metal Ions from Solutions |
| title_sort | screening for microbial metal chelating siderophores for the removal of metal ions from solutions |
| topic | metallophore screening CAS assay immobilization metal binding metal revocery |
| url | https://www.mdpi.com/2076-2607/9/1/111 |
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