Efficacy of Denture Cleansers on Microbial Adherence and Surface Topography of Conventional and CAD/CAM-Processed Denture Base Resins
This study assessed the efficacy of five denture cleansers on the microbial adherence and surface topography of conventional and CAD/CAM denture base resins. Acrylic resin discs were fabricated using conventional, milling, and 3D printing methods (N = 180). The discs were contaminated with dual spec...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-01-01
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| Series: | Polymers |
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| Online Access: | https://www.mdpi.com/2073-4360/15/2/460 |
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| author | Afnan F. Alfouzan Malath Tuwaym Ebtihal N. Aldaghri Tagreed Alojaymi Hadeel Minife Alotiabi Sara M. Al Taweel Hanan N. Al-Otaibi Rizwan Ali Huda Alshehri Nawaf Labban |
| author_facet | Afnan F. Alfouzan Malath Tuwaym Ebtihal N. Aldaghri Tagreed Alojaymi Hadeel Minife Alotiabi Sara M. Al Taweel Hanan N. Al-Otaibi Rizwan Ali Huda Alshehri Nawaf Labban |
| author_sort | Afnan F. Alfouzan |
| collection | DOAJ |
| description | This study assessed the efficacy of five denture cleansers on the microbial adherence and surface topography of conventional and CAD/CAM denture base resins. Acrylic resin discs were fabricated using conventional, milling, and 3D printing methods (N = 180). The discs were contaminated with dual species of <i>Candida albicans</i> and <i>Streptococcus mutans</i> biofilm for 72 h and then disinfected with either of the denture cleansers (Fittydent cleansing tablets, 2% Chlorhexidine gluconate, 0.2% Chlorhexidine gluconate, 0.5% sodium hypochlorite, and 1% sodium hypochlorite (n = 10). Distilled water served as the control group. The colony-forming units of the microorganisms were calculated, followed by post-treatment surface roughness. Data were statistically analyzed using one-way ANOVA, paired <i>t</i>-test, and post hoc Tukey HSD test (α = 0.05). Among the denture cleansers, 2% Chlorhexidine gluconate, 0.5% sodium hypochlorite, and 1% sodium hypochlorite had the best cleansing effect on the resin discs and demonstrated zero growth of colonies for both the species. Comparing the material groups, the 3D-processed discs showed higher colony-forming units followed by the conventional and CAD/CAM milled group. The highest surface roughness was demonstrated by the 3D-printed discs (0.690 ± 0.08 μm), followed by the conventional (0.493 ± 0.11 μm) and the milled groups (0.301 ± 0.08 μm). The tested chemical denture cleansers affected the <i>Candida albicans</i> and <i>Streptococcus mutans</i> adhesion compared to control discs immersed in distilled water. The clinician may recommend to their patient to use 2% chlorhexidine gluconate for the disinfection of CAD/CAM PMMA denture base materials. |
| first_indexed | 2024-03-09T11:23:20Z |
| format | Article |
| id | doaj.art-5a162e5804074bd1a726edded7c4e6ec |
| institution | Directory Open Access Journal |
| issn | 2073-4360 |
| language | English |
| last_indexed | 2024-03-09T11:23:20Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Polymers |
| spelling | doaj.art-5a162e5804074bd1a726edded7c4e6ec2023-12-01T00:10:29ZengMDPI AGPolymers2073-43602023-01-0115246010.3390/polym15020460Efficacy of Denture Cleansers on Microbial Adherence and Surface Topography of Conventional and CAD/CAM-Processed Denture Base ResinsAfnan F. Alfouzan0Malath Tuwaym1Ebtihal N. Aldaghri2Tagreed Alojaymi3Hadeel Minife Alotiabi4Sara M. Al Taweel5Hanan N. Al-Otaibi6Rizwan Ali7Huda Alshehri8Nawaf Labban9Department of Prosthetic Dental Sciences, College of Dentistry, King Saud University, Riyadh 11545, Saudi ArabiaCollege of Dentistry, King Saud University, P.O. Box 60169, Riyadh 11545, Saudi ArabiaCollege of Dentistry, King Saud University, P.O. Box 60169, Riyadh 11545, Saudi ArabiaCollege of Dentistry, King Saud University, P.O. Box 60169, Riyadh 11545, Saudi ArabiaDepartment of Prosthetic Dental Sciences, College of Dentistry, King Saud Bin Abdulaziz University for Health Sciences, Riyadh 14611, Saudi ArabiaDepartment of Prosthetic Dental Sciences, College of Dentistry, King Saud University, Riyadh 11545, Saudi ArabiaDepartment of Prosthetic Dental Sciences, College of Dentistry, King Saud University, Riyadh 11545, Saudi ArabiaMedical Research Core Facility and Platforms, King Abdullah International Medical Research Center, King Saud Bin Abdulaziz University for Health Sciences, Ministry of National Guard Health Affairs, Riyadh 14611, Saudi ArabiaDepartment of Prosthetic Dental Sciences, College of Dentistry, King Saud University, Riyadh 11545, Saudi ArabiaDepartment of Prosthetic Dental Sciences, College of Dentistry, King Saud University, Riyadh 11545, Saudi ArabiaThis study assessed the efficacy of five denture cleansers on the microbial adherence and surface topography of conventional and CAD/CAM denture base resins. Acrylic resin discs were fabricated using conventional, milling, and 3D printing methods (N = 180). The discs were contaminated with dual species of <i>Candida albicans</i> and <i>Streptococcus mutans</i> biofilm for 72 h and then disinfected with either of the denture cleansers (Fittydent cleansing tablets, 2% Chlorhexidine gluconate, 0.2% Chlorhexidine gluconate, 0.5% sodium hypochlorite, and 1% sodium hypochlorite (n = 10). Distilled water served as the control group. The colony-forming units of the microorganisms were calculated, followed by post-treatment surface roughness. Data were statistically analyzed using one-way ANOVA, paired <i>t</i>-test, and post hoc Tukey HSD test (α = 0.05). Among the denture cleansers, 2% Chlorhexidine gluconate, 0.5% sodium hypochlorite, and 1% sodium hypochlorite had the best cleansing effect on the resin discs and demonstrated zero growth of colonies for both the species. Comparing the material groups, the 3D-processed discs showed higher colony-forming units followed by the conventional and CAD/CAM milled group. The highest surface roughness was demonstrated by the 3D-printed discs (0.690 ± 0.08 μm), followed by the conventional (0.493 ± 0.11 μm) and the milled groups (0.301 ± 0.08 μm). The tested chemical denture cleansers affected the <i>Candida albicans</i> and <i>Streptococcus mutans</i> adhesion compared to control discs immersed in distilled water. The clinician may recommend to their patient to use 2% chlorhexidine gluconate for the disinfection of CAD/CAM PMMA denture base materials.https://www.mdpi.com/2073-4360/15/2/4603D printingCAD/CAM technologiespolymethylmethacrylateacrylic resinsdenture cleansers |
| spellingShingle | Afnan F. Alfouzan Malath Tuwaym Ebtihal N. Aldaghri Tagreed Alojaymi Hadeel Minife Alotiabi Sara M. Al Taweel Hanan N. Al-Otaibi Rizwan Ali Huda Alshehri Nawaf Labban Efficacy of Denture Cleansers on Microbial Adherence and Surface Topography of Conventional and CAD/CAM-Processed Denture Base Resins Polymers 3D printing CAD/CAM technologies polymethylmethacrylate acrylic resins denture cleansers |
| title | Efficacy of Denture Cleansers on Microbial Adherence and Surface Topography of Conventional and CAD/CAM-Processed Denture Base Resins |
| title_full | Efficacy of Denture Cleansers on Microbial Adherence and Surface Topography of Conventional and CAD/CAM-Processed Denture Base Resins |
| title_fullStr | Efficacy of Denture Cleansers on Microbial Adherence and Surface Topography of Conventional and CAD/CAM-Processed Denture Base Resins |
| title_full_unstemmed | Efficacy of Denture Cleansers on Microbial Adherence and Surface Topography of Conventional and CAD/CAM-Processed Denture Base Resins |
| title_short | Efficacy of Denture Cleansers on Microbial Adherence and Surface Topography of Conventional and CAD/CAM-Processed Denture Base Resins |
| title_sort | efficacy of denture cleansers on microbial adherence and surface topography of conventional and cad cam processed denture base resins |
| topic | 3D printing CAD/CAM technologies polymethylmethacrylate acrylic resins denture cleansers |
| url | https://www.mdpi.com/2073-4360/15/2/460 |
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