Osteogenic growth peptide enhances osteogenic differentiation of human periodontal ligament stem cells
Bone tissue engineering consists of three major components namely cells, scaffolds, and signaling molecules to improve bone regeneration. These integrated principles can be applied in patients suffered from bone resorption diseases, such as osteoporosis and periodontitis. Osteogenic growth peptide (...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-07-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844022012245 |
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| author | Steven Dwi Purbantoro Thanaphum Osathanon Sirirat Nantavisai Chenphop Sawangmake |
| author_facet | Steven Dwi Purbantoro Thanaphum Osathanon Sirirat Nantavisai Chenphop Sawangmake |
| author_sort | Steven Dwi Purbantoro |
| collection | DOAJ |
| description | Bone tissue engineering consists of three major components namely cells, scaffolds, and signaling molecules to improve bone regeneration. These integrated principles can be applied in patients suffered from bone resorption diseases, such as osteoporosis and periodontitis. Osteogenic growth peptide (OGP) is a fourteen-amino acid sequence peptide that has the potential to regenerate bone tissues. This study aimed to disseminate the osteogenic differentiation of human periodontal ligament stem cells (hPDLSCs) with OGP treatment. OGP was elaborated for proliferation, cytotoxicity, osteogenic differentiation effects, and the involvement of osteogenic related signaling pathways in vitro. This study found that OGP at lower concentration shows better effects on cytotoxicity and proliferation. Moreover, OGP at concentration 0.01 nM had the most potential to differentiate hPDLSCs toward osteogenic lineage comparing with higher concentrations of OGP. The phenomenon was mainly involving transforming growth factor-beta (TGF-β), bone morphogenetic protein (BMP), Hedgehog, and Wingless-related (Wnt) pathways. Further, SB-431542 treatment demonstrated the partial involvement of OGP in regulating osteogenic differentiation of hPDLSCs. In conclusion, OGP at low concentration enhances osteogenic differentiation of hPDLSCs by governing TGF-β signaling pathway. |
| first_indexed | 2024-12-10T21:18:13Z |
| format | Article |
| id | doaj.art-5a16da62c6cf4d14be31509cdaee0df9 |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-12-10T21:18:13Z |
| publishDate | 2022-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-5a16da62c6cf4d14be31509cdaee0df92022-12-22T01:33:13ZengElsevierHeliyon2405-84402022-07-0187e09936Osteogenic growth peptide enhances osteogenic differentiation of human periodontal ligament stem cellsSteven Dwi Purbantoro0Thanaphum Osathanon1Sirirat Nantavisai2Chenphop Sawangmake3The International Graduate Program of Veterinary Science and Technology (VST), Faculty of Veterinary Science, Chulalongkorn University, Bangkok, 10330, Thailand; Veterinary Stem Cell and Bioengineering Innovation Center (VSCBIC), Faculty of Veterinary Science, Chulalongkorn University, Bangkok, 10330, Thailand; Veterinary Stem Cell and Bioengineering Research Unit, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, 10330, ThailandDepartment of Anatomy, Faculty of Dentistry, Chulalongkorn University, Bangkok, 10330, Thailand; Dental Stem Cell Biology Research Unit, Faculty of Dentistry, Chulalongkorn University, Bangkok, 10330, ThailandVeterinary Stem Cell and Bioengineering Innovation Center (VSCBIC), Faculty of Veterinary Science, Chulalongkorn University, Bangkok, 10330, Thailand; Veterinary Stem Cell and Bioengineering Research Unit, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, 10330, Thailand; Academic Affairs, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, 10330, Thailand; Corresponding author.Veterinary Stem Cell and Bioengineering Innovation Center (VSCBIC), Faculty of Veterinary Science, Chulalongkorn University, Bangkok, 10330, Thailand; Veterinary Stem Cell and Bioengineering Research Unit, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, 10330, Thailand; Department of Pharmacology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, 10330, Thailand; Center of Excellence in Regenerative Dentistry, Faculty of Dentistry, Chulalongkorn University, Bangkok, 10330, Thailand; Corresponding author.Bone tissue engineering consists of three major components namely cells, scaffolds, and signaling molecules to improve bone regeneration. These integrated principles can be applied in patients suffered from bone resorption diseases, such as osteoporosis and periodontitis. Osteogenic growth peptide (OGP) is a fourteen-amino acid sequence peptide that has the potential to regenerate bone tissues. This study aimed to disseminate the osteogenic differentiation of human periodontal ligament stem cells (hPDLSCs) with OGP treatment. OGP was elaborated for proliferation, cytotoxicity, osteogenic differentiation effects, and the involvement of osteogenic related signaling pathways in vitro. This study found that OGP at lower concentration shows better effects on cytotoxicity and proliferation. Moreover, OGP at concentration 0.01 nM had the most potential to differentiate hPDLSCs toward osteogenic lineage comparing with higher concentrations of OGP. The phenomenon was mainly involving transforming growth factor-beta (TGF-β), bone morphogenetic protein (BMP), Hedgehog, and Wingless-related (Wnt) pathways. Further, SB-431542 treatment demonstrated the partial involvement of OGP in regulating osteogenic differentiation of hPDLSCs. In conclusion, OGP at low concentration enhances osteogenic differentiation of hPDLSCs by governing TGF-β signaling pathway.http://www.sciencedirect.com/science/article/pii/S2405844022012245Osteogenic growth peptide (OGP)Human periodontal ligament stem cells (hPDLSCs)Osteogenic differentiationBone regeneration |
| spellingShingle | Steven Dwi Purbantoro Thanaphum Osathanon Sirirat Nantavisai Chenphop Sawangmake Osteogenic growth peptide enhances osteogenic differentiation of human periodontal ligament stem cells Heliyon Osteogenic growth peptide (OGP) Human periodontal ligament stem cells (hPDLSCs) Osteogenic differentiation Bone regeneration |
| title | Osteogenic growth peptide enhances osteogenic differentiation of human periodontal ligament stem cells |
| title_full | Osteogenic growth peptide enhances osteogenic differentiation of human periodontal ligament stem cells |
| title_fullStr | Osteogenic growth peptide enhances osteogenic differentiation of human periodontal ligament stem cells |
| title_full_unstemmed | Osteogenic growth peptide enhances osteogenic differentiation of human periodontal ligament stem cells |
| title_short | Osteogenic growth peptide enhances osteogenic differentiation of human periodontal ligament stem cells |
| title_sort | osteogenic growth peptide enhances osteogenic differentiation of human periodontal ligament stem cells |
| topic | Osteogenic growth peptide (OGP) Human periodontal ligament stem cells (hPDLSCs) Osteogenic differentiation Bone regeneration |
| url | http://www.sciencedirect.com/science/article/pii/S2405844022012245 |
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