Distribution of the Water-Soluble Astaxanthin Binding Carotenoprotein (AstaP) in Scenedesmaceae

Photooxidative stress-inducible water-soluble astaxanthin-binding proteins, designated as AstaP, were identified in two Scenedesmaceae strains, <i>Coelastrella astaxanthina</i> Ki-4 and <i>Scenedesmus obtusus</i> Oki-4N; both strains were isolated under high light conditions....

Full description

Bibliographic Details
Main Authors: Hiroki Toyoshima, Ami Miyata, Risako Yoshida, Taichiro Ishige, Shinichi Takaichi, Shinji Kawasaki
Format: Article
Language:English
Published: MDPI AG 2021-06-01
Series:Marine Drugs
Subjects:
Online Access:https://www.mdpi.com/1660-3397/19/6/349
_version_ 1797529419987812352
author Hiroki Toyoshima
Ami Miyata
Risako Yoshida
Taichiro Ishige
Shinichi Takaichi
Shinji Kawasaki
author_facet Hiroki Toyoshima
Ami Miyata
Risako Yoshida
Taichiro Ishige
Shinichi Takaichi
Shinji Kawasaki
author_sort Hiroki Toyoshima
collection DOAJ
description Photooxidative stress-inducible water-soluble astaxanthin-binding proteins, designated as AstaP, were identified in two Scenedesmaceae strains, <i>Coelastrella astaxanthina</i> Ki-4 and <i>Scenedesmus obtusus</i> Oki-4N; both strains were isolated under high light conditions. These AstaPs are classified as a novel family of carotenoprotein and are useful for providing valuable astaxanthin in water-soluble form; however, the distribution of AstaP orthologs in other microalgae remains unknown. Here, we examined the distribution of AstaP orthologs in the family Scenedesmaceae with two model microalgae, <i>Chlamydomonas reinhardtii</i> and <i>Chlorella variabilis</i>. The expression of AstaP orthologs under photooxidative stress conditions was detected in cell extracts of Scenedesmaceae strains, but not in model algal strains. Aqueous orange proteins produced by Scenedesmaceae strains were shown to bind astaxanthin. The protein from <i>Scenedesmus costatus</i> SAG 46.88 was purified. It was named ScosAstaP and found to bind astaxanthin. The deduced amino acid sequence from a gene encoding ScosAstaP showed 62% identity to Ki-4 AstaP. The expression of the genes encoding AstaP orthologs was shown to be inducible under photooxidative stress conditions; however, the production amounts of AstaP orthologs were estimated to be approximately 5 to 10 times lower than that of Ki-4 and Oki-4N.
first_indexed 2024-03-10T10:14:37Z
format Article
id doaj.art-5a18e4eff8144f29a2dfd5a0cba85a3a
institution Directory Open Access Journal
issn 1660-3397
language English
last_indexed 2024-03-10T10:14:37Z
publishDate 2021-06-01
publisher MDPI AG
record_format Article
series Marine Drugs
spelling doaj.art-5a18e4eff8144f29a2dfd5a0cba85a3a2023-11-22T00:57:06ZengMDPI AGMarine Drugs1660-33972021-06-0119634910.3390/md19060349Distribution of the Water-Soluble Astaxanthin Binding Carotenoprotein (AstaP) in ScenedesmaceaeHiroki Toyoshima0Ami Miyata1Risako Yoshida2Taichiro Ishige3Shinichi Takaichi4Shinji Kawasaki5Department of Bioscience, Tokyo University of Agriculture, 1-1-1 Sakuragaoka, Setagaya-ku, Tokyo 156-8502, JapanDepartment of Bioscience, Tokyo University of Agriculture, 1-1-1 Sakuragaoka, Setagaya-ku, Tokyo 156-8502, JapanDepartment of Bioscience, Tokyo University of Agriculture, 1-1-1 Sakuragaoka, Setagaya-ku, Tokyo 156-8502, JapanNODAI Genome Research Centre, Tokyo University of Agriculture, 1-1-1 Sakuragaoka, Setagaya-ku, Tokyo 156-8502, JapanDepartment of Molecular Microbiology, Tokyo University of Agriculture, 1-1-1 Sakuragaoka, Setagaya-ku, Tokyo 156-8502, JapanDepartment of Bioscience, Tokyo University of Agriculture, 1-1-1 Sakuragaoka, Setagaya-ku, Tokyo 156-8502, JapanPhotooxidative stress-inducible water-soluble astaxanthin-binding proteins, designated as AstaP, were identified in two Scenedesmaceae strains, <i>Coelastrella astaxanthina</i> Ki-4 and <i>Scenedesmus obtusus</i> Oki-4N; both strains were isolated under high light conditions. These AstaPs are classified as a novel family of carotenoprotein and are useful for providing valuable astaxanthin in water-soluble form; however, the distribution of AstaP orthologs in other microalgae remains unknown. Here, we examined the distribution of AstaP orthologs in the family Scenedesmaceae with two model microalgae, <i>Chlamydomonas reinhardtii</i> and <i>Chlorella variabilis</i>. The expression of AstaP orthologs under photooxidative stress conditions was detected in cell extracts of Scenedesmaceae strains, but not in model algal strains. Aqueous orange proteins produced by Scenedesmaceae strains were shown to bind astaxanthin. The protein from <i>Scenedesmus costatus</i> SAG 46.88 was purified. It was named ScosAstaP and found to bind astaxanthin. The deduced amino acid sequence from a gene encoding ScosAstaP showed 62% identity to Ki-4 AstaP. The expression of the genes encoding AstaP orthologs was shown to be inducible under photooxidative stress conditions; however, the production amounts of AstaP orthologs were estimated to be approximately 5 to 10 times lower than that of Ki-4 and Oki-4N.https://www.mdpi.com/1660-3397/19/6/349astaxanthin<i>Scenedesmus</i><i>Coelastrella</i>carotenoproteinastaxanthin-binding protein
spellingShingle Hiroki Toyoshima
Ami Miyata
Risako Yoshida
Taichiro Ishige
Shinichi Takaichi
Shinji Kawasaki
Distribution of the Water-Soluble Astaxanthin Binding Carotenoprotein (AstaP) in Scenedesmaceae
Marine Drugs
astaxanthin
<i>Scenedesmus</i>
<i>Coelastrella</i>
carotenoprotein
astaxanthin-binding protein
title Distribution of the Water-Soluble Astaxanthin Binding Carotenoprotein (AstaP) in Scenedesmaceae
title_full Distribution of the Water-Soluble Astaxanthin Binding Carotenoprotein (AstaP) in Scenedesmaceae
title_fullStr Distribution of the Water-Soluble Astaxanthin Binding Carotenoprotein (AstaP) in Scenedesmaceae
title_full_unstemmed Distribution of the Water-Soluble Astaxanthin Binding Carotenoprotein (AstaP) in Scenedesmaceae
title_short Distribution of the Water-Soluble Astaxanthin Binding Carotenoprotein (AstaP) in Scenedesmaceae
title_sort distribution of the water soluble astaxanthin binding carotenoprotein astap in scenedesmaceae
topic astaxanthin
<i>Scenedesmus</i>
<i>Coelastrella</i>
carotenoprotein
astaxanthin-binding protein
url https://www.mdpi.com/1660-3397/19/6/349
work_keys_str_mv AT hirokitoyoshima distributionofthewatersolubleastaxanthinbindingcarotenoproteinastapinscenedesmaceae
AT amimiyata distributionofthewatersolubleastaxanthinbindingcarotenoproteinastapinscenedesmaceae
AT risakoyoshida distributionofthewatersolubleastaxanthinbindingcarotenoproteinastapinscenedesmaceae
AT taichiroishige distributionofthewatersolubleastaxanthinbindingcarotenoproteinastapinscenedesmaceae
AT shinichitakaichi distributionofthewatersolubleastaxanthinbindingcarotenoproteinastapinscenedesmaceae
AT shinjikawasaki distributionofthewatersolubleastaxanthinbindingcarotenoproteinastapinscenedesmaceae