Immunogenic Potential of the Mediterranean Fever Gene in Patients with Coronavirus Disease: A Cross-Sectional Study
Background: In December 2019, an outbreak of pneumonia caused by the novel coronavirus disease 2019 (COVID-19) became a pandemic and caused a global health crisis. This study evaluates the immunogenic potential of the Mediterranean fever (MEFV) gene in patients with COVID-19. Methods: A cross-sectio...
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| Format: | Article |
| Language: | English |
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Shiraz University of Medical Sciences
2023-01-01
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| Series: | Iranian Journal of Medical Sciences |
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| Online Access: | https://ijms.sums.ac.ir/article_48719_a38ff432a05bcb480b00b0cc118ca4c7.pdf |
| _version_ | 1811177150436343808 |
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| author | Farhad Salehzadeh Farhad Pourfarzi Rasool Molatefi Behzad Davarnia Ehsan Shahbazfar Farzad Ahmadabadi |
| author_facet | Farhad Salehzadeh Farhad Pourfarzi Rasool Molatefi Behzad Davarnia Ehsan Shahbazfar Farzad Ahmadabadi |
| author_sort | Farhad Salehzadeh |
| collection | DOAJ |
| description | Background: In December 2019, an outbreak of pneumonia caused by the novel coronavirus disease 2019 (COVID-19) became a pandemic and caused a global health crisis. This study evaluates the immunogenic potential of the Mediterranean fever (MEFV) gene in patients with COVID-19. Methods: A cross-sectional study was conducted from March to April 2020 in various COVID-19 referral centers in Ardabil, Iran. Blood samples of 50 hospitalized patients with confirmed COVID-19 were evaluated for MEFV gene mutation using the amplification refractory mutation system polymerase chain reaction (ARMS-PCR) and Sanger sequencing. Statistical analysis was performed using SPSS software, version 22.0.Results: Mutations of the MEFV gene were found in 6 (12%) of the patients. All mutations were heterozygous, and no homozygous or compound heterozygous forms were detected. The total mutant allele frequency was 6% and the carrier rate was 12%. The most common allele of the MEFV variant was E148Q, detected in 3 (6%) patients. No mutant variant of the MEFV gene was detected in deceased patients. None of the mutation carriers had familial Mediterranean fever (FMF) symptoms or a family history of FMF.Conclusion: MEFV gene mutations may have immunogenic potential in patients with COVID-19.A preprint version of this article has already been published at https://www.researchsquare.com/article/rs-69373/latest.pdf. |
| first_indexed | 2024-04-10T22:56:49Z |
| format | Article |
| id | doaj.art-5a1c109d6f6849b18216ea22e7fcf728 |
| institution | Directory Open Access Journal |
| issn | 0253-0716 1735-3688 |
| language | English |
| last_indexed | 2024-04-10T22:56:49Z |
| publishDate | 2023-01-01 |
| publisher | Shiraz University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Medical Sciences |
| spelling | doaj.art-5a1c109d6f6849b18216ea22e7fcf7282023-01-14T06:21:07ZengShiraz University of Medical SciencesIranian Journal of Medical Sciences0253-07161735-36882023-01-01481434810.30476/ijms.2022.92802.240848719Immunogenic Potential of the Mediterranean Fever Gene in Patients with Coronavirus Disease: A Cross-Sectional StudyFarhad Salehzadeh0Farhad Pourfarzi1Rasool Molatefi2Behzad Davarnia3Ehsan Shahbazfar4Farzad Ahmadabadi5Department of Pediatric, Bouali Children’s Hospital, Ardabil University of Medical Sciences, Ardabil, IranDepartment of Community Medicine, School of Medicine, Ardabil University of Medical Sciences, Ardabil, IranDepartment of Pediatric, Bouali Children’s Hospital, Ardabil University of Medical Sciences, Ardabil, IranDepartment of Genetic, School of Medicine, Ardabil University of Medical Sciences, Ardabil, IranDepartment of Pediatric, Bouali Children’s Hospital, Ardabil University of Medical Sciences, Ardabil, IranDepartment of Pediatric, Bouali Children’s Hospital, Ardabil University of Medical Sciences, Ardabil, IranBackground: In December 2019, an outbreak of pneumonia caused by the novel coronavirus disease 2019 (COVID-19) became a pandemic and caused a global health crisis. This study evaluates the immunogenic potential of the Mediterranean fever (MEFV) gene in patients with COVID-19. Methods: A cross-sectional study was conducted from March to April 2020 in various COVID-19 referral centers in Ardabil, Iran. Blood samples of 50 hospitalized patients with confirmed COVID-19 were evaluated for MEFV gene mutation using the amplification refractory mutation system polymerase chain reaction (ARMS-PCR) and Sanger sequencing. Statistical analysis was performed using SPSS software, version 22.0.Results: Mutations of the MEFV gene were found in 6 (12%) of the patients. All mutations were heterozygous, and no homozygous or compound heterozygous forms were detected. The total mutant allele frequency was 6% and the carrier rate was 12%. The most common allele of the MEFV variant was E148Q, detected in 3 (6%) patients. No mutant variant of the MEFV gene was detected in deceased patients. None of the mutation carriers had familial Mediterranean fever (FMF) symptoms or a family history of FMF.Conclusion: MEFV gene mutations may have immunogenic potential in patients with COVID-19.A preprint version of this article has already been published at https://www.researchsquare.com/article/rs-69373/latest.pdf.https://ijms.sums.ac.ir/article_48719_a38ff432a05bcb480b00b0cc118ca4c7.pdfcovid-19familial mediterranean fevermefv gene |
| spellingShingle | Farhad Salehzadeh Farhad Pourfarzi Rasool Molatefi Behzad Davarnia Ehsan Shahbazfar Farzad Ahmadabadi Immunogenic Potential of the Mediterranean Fever Gene in Patients with Coronavirus Disease: A Cross-Sectional Study Iranian Journal of Medical Sciences covid-19 familial mediterranean fever mefv gene |
| title | Immunogenic Potential of the Mediterranean Fever Gene in Patients with Coronavirus Disease: A Cross-Sectional Study |
| title_full | Immunogenic Potential of the Mediterranean Fever Gene in Patients with Coronavirus Disease: A Cross-Sectional Study |
| title_fullStr | Immunogenic Potential of the Mediterranean Fever Gene in Patients with Coronavirus Disease: A Cross-Sectional Study |
| title_full_unstemmed | Immunogenic Potential of the Mediterranean Fever Gene in Patients with Coronavirus Disease: A Cross-Sectional Study |
| title_short | Immunogenic Potential of the Mediterranean Fever Gene in Patients with Coronavirus Disease: A Cross-Sectional Study |
| title_sort | immunogenic potential of the mediterranean fever gene in patients with coronavirus disease a cross sectional study |
| topic | covid-19 familial mediterranean fever mefv gene |
| url | https://ijms.sums.ac.ir/article_48719_a38ff432a05bcb480b00b0cc118ca4c7.pdf |
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