Ultrasonic extraction, structural modification and gastric mucosal cells protective activity of a polysaccharide from Dendrobium denneanum
Optimize the ultrasonic extraction process of polysaccharides from Dendrobium denneanum by Response surface design (RSM). The main polysaccharide (DP40) from was separated by anion exchange chromatography, and its structure was modified by low-temperature plasma. The results showed that the polysacc...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-09-01
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| Series: | Arabian Journal of Chemistry |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1878535223004951 |
| _version_ | 1797776649654108160 |
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| author | Yijun Fan Jie Ma Gang Wang Xuebing Li Yuanyuan Liu Erya Xu Aoxue Luo |
| author_facet | Yijun Fan Jie Ma Gang Wang Xuebing Li Yuanyuan Liu Erya Xu Aoxue Luo |
| author_sort | Yijun Fan |
| collection | DOAJ |
| description | Optimize the ultrasonic extraction process of polysaccharides from Dendrobium denneanum by Response surface design (RSM). The main polysaccharide (DP40) from was separated by anion exchange chromatography, and its structure was modified by low-temperature plasma. The results showed that the polysaccharide extraction rate of ultrasonic was 22.98 ± 0.97%, which was significantly higher than water extraction method(P < 0.05). FT-IR showed that low-temperature plasma modification could improve the water solubility and OH content. NMR showed that the 1 → 6 glycosidic bonds of DP40 are transformed into 1 → 4 glycosidic bonds and 1 → 3 glycosidic bonds, and the α-configuration sugar ring is transformed into β-configuration after plasma treatment. SEM and TEM analysis showed that the morphology of modified DP40 changed from layered porous network structure to smooth morphology, and bulged into semicircular particles, and the degree of cross-linking of surface molecules increased. The results of biological activity experiments indicated that the modified polysaccharide (500–1000 μg·mL−1) can significantly improve the survival rate of GES-1 cells (p < 0.01). Compared with the untreated group, the high concentration of 1000 μg·mL−1 DP40-plasma could not only reduce ROS by 14.5% (p < 0.05) and MDA by 27.45% (p < 0.05), increase SOD activity by 28.27%, but also reduce cytokines (IL-8, TNF-α, IL-1β) by 31.82% (p < 0.05), 33.23% (p < 0.05) and 21.97% (p < 0.05), respectively. At the same time, low-temperature plasma modification can effectively reduce the caspase 3 activity, prevent cell apoptosis, and protect gastric mucosal cells from ethanol damage. Therefore, low-temperature plasma modification can improve the biological activity of DP40 polysaccharide by altering its structural characteristics. The results provide a new idea for the application of low-temperature plasma modification in biomacromolecules. |
| first_indexed | 2024-03-12T22:53:56Z |
| format | Article |
| id | doaj.art-5a1cd36fa68842569da212a27d111f2f |
| institution | Directory Open Access Journal |
| issn | 1878-5352 |
| language | English |
| last_indexed | 2024-03-12T22:53:56Z |
| publishDate | 2023-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Arabian Journal of Chemistry |
| spelling | doaj.art-5a1cd36fa68842569da212a27d111f2f2023-07-20T04:37:56ZengElsevierArabian Journal of Chemistry1878-53522023-09-01169105033Ultrasonic extraction, structural modification and gastric mucosal cells protective activity of a polysaccharide from Dendrobium denneanumYijun Fan0Jie Ma1Gang Wang2Xuebing Li3Yuanyuan Liu4Erya Xu5Aoxue Luo6Department of Landscape Plants, Sichuan Agricultural University, Chengdu 611130, China; Corresponding authors.Department of Landscape Plants, Sichuan Agricultural University, Chengdu 611130, ChinaCollege of Forest, Sichuan Agricultural University, Chengdu 611130, ChinaDepartment of Landscape Plants, Sichuan Agricultural University, Chengdu 611130, ChinaDepartment of Landscape Plants, Sichuan Agricultural University, Chengdu 611130, ChinaDepartment of Landscape Plants, Sichuan Agricultural University, Chengdu 611130, ChinaDepartment of Landscape Plants, Sichuan Agricultural University, Chengdu 611130, China; Corresponding authors.Optimize the ultrasonic extraction process of polysaccharides from Dendrobium denneanum by Response surface design (RSM). The main polysaccharide (DP40) from was separated by anion exchange chromatography, and its structure was modified by low-temperature plasma. The results showed that the polysaccharide extraction rate of ultrasonic was 22.98 ± 0.97%, which was significantly higher than water extraction method(P < 0.05). FT-IR showed that low-temperature plasma modification could improve the water solubility and OH content. NMR showed that the 1 → 6 glycosidic bonds of DP40 are transformed into 1 → 4 glycosidic bonds and 1 → 3 glycosidic bonds, and the α-configuration sugar ring is transformed into β-configuration after plasma treatment. SEM and TEM analysis showed that the morphology of modified DP40 changed from layered porous network structure to smooth morphology, and bulged into semicircular particles, and the degree of cross-linking of surface molecules increased. The results of biological activity experiments indicated that the modified polysaccharide (500–1000 μg·mL−1) can significantly improve the survival rate of GES-1 cells (p < 0.01). Compared with the untreated group, the high concentration of 1000 μg·mL−1 DP40-plasma could not only reduce ROS by 14.5% (p < 0.05) and MDA by 27.45% (p < 0.05), increase SOD activity by 28.27%, but also reduce cytokines (IL-8, TNF-α, IL-1β) by 31.82% (p < 0.05), 33.23% (p < 0.05) and 21.97% (p < 0.05), respectively. At the same time, low-temperature plasma modification can effectively reduce the caspase 3 activity, prevent cell apoptosis, and protect gastric mucosal cells from ethanol damage. Therefore, low-temperature plasma modification can improve the biological activity of DP40 polysaccharide by altering its structural characteristics. The results provide a new idea for the application of low-temperature plasma modification in biomacromolecules.http://www.sciencedirect.com/science/article/pii/S1878535223004951Ultrasonic extractionDendrobium denneanumPolysaccharideLow-temperature plasmaStructural characteristicsGES-1 cells |
| spellingShingle | Yijun Fan Jie Ma Gang Wang Xuebing Li Yuanyuan Liu Erya Xu Aoxue Luo Ultrasonic extraction, structural modification and gastric mucosal cells protective activity of a polysaccharide from Dendrobium denneanum Arabian Journal of Chemistry Ultrasonic extraction Dendrobium denneanum Polysaccharide Low-temperature plasma Structural characteristics GES-1 cells |
| title | Ultrasonic extraction, structural modification and gastric mucosal cells protective activity of a polysaccharide from Dendrobium denneanum |
| title_full | Ultrasonic extraction, structural modification and gastric mucosal cells protective activity of a polysaccharide from Dendrobium denneanum |
| title_fullStr | Ultrasonic extraction, structural modification and gastric mucosal cells protective activity of a polysaccharide from Dendrobium denneanum |
| title_full_unstemmed | Ultrasonic extraction, structural modification and gastric mucosal cells protective activity of a polysaccharide from Dendrobium denneanum |
| title_short | Ultrasonic extraction, structural modification and gastric mucosal cells protective activity of a polysaccharide from Dendrobium denneanum |
| title_sort | ultrasonic extraction structural modification and gastric mucosal cells protective activity of a polysaccharide from dendrobium denneanum |
| topic | Ultrasonic extraction Dendrobium denneanum Polysaccharide Low-temperature plasma Structural characteristics GES-1 cells |
| url | http://www.sciencedirect.com/science/article/pii/S1878535223004951 |
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