Ulinastatin: A Potential Alternative to Glucocorticoid in the Treatment of Severe Decompression Sickness
Inflammatory reaction is the crux in various clinical critical diseases including decompression sickness (DCS). Ulinastatin (UTI), a potent anti-inflammatory agent, has been used clinically, including as a substitution for steroids. This study aimed to explore the potential effects of UTI upon DCS i...
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Frontiers Media S.A.
2020-03-01
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| Series: | Frontiers in Physiology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fphys.2020.00273/full |
| _version_ | 1819108811091214336 |
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| author | Wen-tao Meng Long Qing Chun-zhen Li Kun Zhang Hong-jie Yi Xu-peng Zhao Wei-gang Xu |
| author_facet | Wen-tao Meng Long Qing Chun-zhen Li Kun Zhang Hong-jie Yi Xu-peng Zhao Wei-gang Xu |
| author_sort | Wen-tao Meng |
| collection | DOAJ |
| description | Inflammatory reaction is the crux in various clinical critical diseases including decompression sickness (DCS). Ulinastatin (UTI), a potent anti-inflammatory agent, has been used clinically, including as a substitution for steroids. This study aimed to explore the potential effects of UTI upon DCS in a rabbit model. Eighty-eight rabbits were subjected to simulated diving to 6 atmospheres absolute (ATA) for 60 min with 2.5-minute decompression. Three doses of UTI (15/7.5/3.75 × 105 U/kg) or saline were intravenously administered immediately following decompression. Circulating bubbles were monitored for 3 h following decompression and DCS signs were evaluated for 24 h. Blood was sampled 8 times during 72 h after decompression for inflammatory, endothelial, oxidative and routine blood indices. Lung tissues were also sampled for evaluating endothelial function. Another six rabbits were used as Normal controls. In the high dose UTI group the mortality, general morbidity and incidence of severe DCS was decreased from 31.25 to 9.38% (P = 0.030), 84.38 to 62.50% (P = 0.048) and 46.88 to 21.88% (P = 0.035), respectively. The high dose of UTI significantly postponed the occurrence of DCS (P = 0.030) and prolonged survival time (P = 0.009) compared with the Saline group, and significantly ameliorated inflammation responses, endothelial injuries and oxidative damage. The results strongly suggest the benefit of UTI on DCS, especially for severe cases. Large doses are needed to achieve significant effects. UTI may be a potential ideal pharmacological candidate for the treatment of severe DCS. |
| first_indexed | 2024-12-22T03:15:51Z |
| format | Article |
| id | doaj.art-5a1d490dc724418d8dbad494276aed60 |
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| issn | 1664-042X |
| language | English |
| last_indexed | 2024-12-22T03:15:51Z |
| publishDate | 2020-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Physiology |
| spelling | doaj.art-5a1d490dc724418d8dbad494276aed602022-12-21T18:40:50ZengFrontiers Media S.A.Frontiers in Physiology1664-042X2020-03-011110.3389/fphys.2020.00273531829Ulinastatin: A Potential Alternative to Glucocorticoid in the Treatment of Severe Decompression SicknessWen-tao Meng0Long Qing1Chun-zhen Li2Kun Zhang3Hong-jie Yi4Xu-peng Zhao5Wei-gang Xu6Department of Diving and Hyperbaric Medicine, Naval Special Medicine Center, Naval Medical University, Shanghai, ChinaNaval Diving Medical Discipline, Naval Special Medicine Center, Naval Medical University, Shanghai, ChinaSchool of Basic Medicines, Naval Medical University, Shanghai, ChinaDepartment of Diving and Hyperbaric Medicine, Naval Special Medicine Center, Naval Medical University, Shanghai, ChinaDepartment of Diving and Hyperbaric Medicine, Naval Special Medicine Center, Naval Medical University, Shanghai, ChinaDepartment of Diving and Hyperbaric Medicine, Naval Special Medicine Center, Naval Medical University, Shanghai, ChinaDepartment of Diving and Hyperbaric Medicine, Naval Special Medicine Center, Naval Medical University, Shanghai, ChinaInflammatory reaction is the crux in various clinical critical diseases including decompression sickness (DCS). Ulinastatin (UTI), a potent anti-inflammatory agent, has been used clinically, including as a substitution for steroids. This study aimed to explore the potential effects of UTI upon DCS in a rabbit model. Eighty-eight rabbits were subjected to simulated diving to 6 atmospheres absolute (ATA) for 60 min with 2.5-minute decompression. Three doses of UTI (15/7.5/3.75 × 105 U/kg) or saline were intravenously administered immediately following decompression. Circulating bubbles were monitored for 3 h following decompression and DCS signs were evaluated for 24 h. Blood was sampled 8 times during 72 h after decompression for inflammatory, endothelial, oxidative and routine blood indices. Lung tissues were also sampled for evaluating endothelial function. Another six rabbits were used as Normal controls. In the high dose UTI group the mortality, general morbidity and incidence of severe DCS was decreased from 31.25 to 9.38% (P = 0.030), 84.38 to 62.50% (P = 0.048) and 46.88 to 21.88% (P = 0.035), respectively. The high dose of UTI significantly postponed the occurrence of DCS (P = 0.030) and prolonged survival time (P = 0.009) compared with the Saline group, and significantly ameliorated inflammation responses, endothelial injuries and oxidative damage. The results strongly suggest the benefit of UTI on DCS, especially for severe cases. Large doses are needed to achieve significant effects. UTI may be a potential ideal pharmacological candidate for the treatment of severe DCS.https://www.frontiersin.org/article/10.3389/fphys.2020.00273/fullulinastatindecompression sicknessinflammationendothelial injuryrabbit |
| spellingShingle | Wen-tao Meng Long Qing Chun-zhen Li Kun Zhang Hong-jie Yi Xu-peng Zhao Wei-gang Xu Ulinastatin: A Potential Alternative to Glucocorticoid in the Treatment of Severe Decompression Sickness Frontiers in Physiology ulinastatin decompression sickness inflammation endothelial injury rabbit |
| title | Ulinastatin: A Potential Alternative to Glucocorticoid in the Treatment of Severe Decompression Sickness |
| title_full | Ulinastatin: A Potential Alternative to Glucocorticoid in the Treatment of Severe Decompression Sickness |
| title_fullStr | Ulinastatin: A Potential Alternative to Glucocorticoid in the Treatment of Severe Decompression Sickness |
| title_full_unstemmed | Ulinastatin: A Potential Alternative to Glucocorticoid in the Treatment of Severe Decompression Sickness |
| title_short | Ulinastatin: A Potential Alternative to Glucocorticoid in the Treatment of Severe Decompression Sickness |
| title_sort | ulinastatin a potential alternative to glucocorticoid in the treatment of severe decompression sickness |
| topic | ulinastatin decompression sickness inflammation endothelial injury rabbit |
| url | https://www.frontiersin.org/article/10.3389/fphys.2020.00273/full |
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