Biological Potential of the Main Component, Thymoquinone, of <i>Nigella sativa</i> in Pulp Therapy—In Vitro Study
This work is designed to assess the antimicrobial efficacy, chelating efficacy, and dissolving capability of the bioactive agent of the <i>Nigella sativa</i> plant (thymoquinone). Four freeze-dried microorganisms were studied. Each species was treated with either 6% sodium Hypochlorite,...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-09-01
|
| Series: | Life |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2075-1729/12/9/1434 |
| _version_ | 1827659434436853760 |
|---|---|
| author | Rana A. Alamoudi Soha A. Alamoudi Ruaa A. Alamoudi |
| author_facet | Rana A. Alamoudi Soha A. Alamoudi Ruaa A. Alamoudi |
| author_sort | Rana A. Alamoudi |
| collection | DOAJ |
| description | This work is designed to assess the antimicrobial efficacy, chelating efficacy, and dissolving capability of the bioactive agent of the <i>Nigella sativa</i> plant (thymoquinone). Four freeze-dried microorganisms were studied. Each species was treated with either 6% sodium Hypochlorite, thymoquinone, or sterile water. The zone of inhibition was measured. Thirty extracted human premolar teeth were utilized to evaluate the smear layer removal. Root canals were mechanically instrumented and then irrigated with either 6% sodium Hypochlorite, 17% ethylenediaminetetraacetic acid, or thymoquinone for 1 min and scanned under the scanning electron microscopic to evaluate the cleanliness of the root canal and the remainder of the smear layer. To evaluate the tissue dissolving effect, Bovine Pulp Tissue was utilized. Randomly treated samples included: 6% sodium Hypochlorite, thymoquinone, or isotonic saline for 30 min. The remaining pulp tissue was weighed. Thymoquinone reported the highest inhibition of microbial multiplication compared to other irrigants (<i>p</i> < 0.001). Thymoquinone solution had an excellent antibacterial effect on endodontic pathogen and did not affect the inorganic and organic tissue inside the root canal. Meanwhile, it reported weak chelating and dissolving effects. Tissue dissolution was statistically significant with sodium Hypochlorite solution compared to other groups (<i>p</i> < 0.001). |
| first_indexed | 2024-03-09T23:22:23Z |
| format | Article |
| id | doaj.art-5a1f1114b18647c6836a44b7e44feed3 |
| institution | Directory Open Access Journal |
| issn | 2075-1729 |
| language | English |
| last_indexed | 2024-03-09T23:22:23Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Life |
| spelling | doaj.art-5a1f1114b18647c6836a44b7e44feed32023-11-23T17:24:05ZengMDPI AGLife2075-17292022-09-01129143410.3390/life12091434Biological Potential of the Main Component, Thymoquinone, of <i>Nigella sativa</i> in Pulp Therapy—In Vitro StudyRana A. Alamoudi0Soha A. Alamoudi1Ruaa A. Alamoudi2Pediatric Dentistry Department, Faculty of Dentistry, King Abdulaziz University, Jeddah 21589, Saudi ArabiaBiological Sciences Department, College of Science and Arts, King Abdulaziz University, Rabigh 21911, Saudi ArabiaEndodontic Department, Faculty of Dentistry, King Abdulaziz University, Jeddah 21589, Saudi ArabiaThis work is designed to assess the antimicrobial efficacy, chelating efficacy, and dissolving capability of the bioactive agent of the <i>Nigella sativa</i> plant (thymoquinone). Four freeze-dried microorganisms were studied. Each species was treated with either 6% sodium Hypochlorite, thymoquinone, or sterile water. The zone of inhibition was measured. Thirty extracted human premolar teeth were utilized to evaluate the smear layer removal. Root canals were mechanically instrumented and then irrigated with either 6% sodium Hypochlorite, 17% ethylenediaminetetraacetic acid, or thymoquinone for 1 min and scanned under the scanning electron microscopic to evaluate the cleanliness of the root canal and the remainder of the smear layer. To evaluate the tissue dissolving effect, Bovine Pulp Tissue was utilized. Randomly treated samples included: 6% sodium Hypochlorite, thymoquinone, or isotonic saline for 30 min. The remaining pulp tissue was weighed. Thymoquinone reported the highest inhibition of microbial multiplication compared to other irrigants (<i>p</i> < 0.001). Thymoquinone solution had an excellent antibacterial effect on endodontic pathogen and did not affect the inorganic and organic tissue inside the root canal. Meanwhile, it reported weak chelating and dissolving effects. Tissue dissolution was statistically significant with sodium Hypochlorite solution compared to other groups (<i>p</i> < 0.001).https://www.mdpi.com/2075-1729/12/9/1434<i>Nigella sativa</i>antimicrobial activityroot canalmicroorganismsthymoquinone |
| spellingShingle | Rana A. Alamoudi Soha A. Alamoudi Ruaa A. Alamoudi Biological Potential of the Main Component, Thymoquinone, of <i>Nigella sativa</i> in Pulp Therapy—In Vitro Study Life <i>Nigella sativa</i> antimicrobial activity root canal microorganisms thymoquinone |
| title | Biological Potential of the Main Component, Thymoquinone, of <i>Nigella sativa</i> in Pulp Therapy—In Vitro Study |
| title_full | Biological Potential of the Main Component, Thymoquinone, of <i>Nigella sativa</i> in Pulp Therapy—In Vitro Study |
| title_fullStr | Biological Potential of the Main Component, Thymoquinone, of <i>Nigella sativa</i> in Pulp Therapy—In Vitro Study |
| title_full_unstemmed | Biological Potential of the Main Component, Thymoquinone, of <i>Nigella sativa</i> in Pulp Therapy—In Vitro Study |
| title_short | Biological Potential of the Main Component, Thymoquinone, of <i>Nigella sativa</i> in Pulp Therapy—In Vitro Study |
| title_sort | biological potential of the main component thymoquinone of i nigella sativa i in pulp therapy in vitro study |
| topic | <i>Nigella sativa</i> antimicrobial activity root canal microorganisms thymoquinone |
| url | https://www.mdpi.com/2075-1729/12/9/1434 |
| work_keys_str_mv | AT ranaaalamoudi biologicalpotentialofthemaincomponentthymoquinoneofinigellasativaiinpulptherapyinvitrostudy AT sohaaalamoudi biologicalpotentialofthemaincomponentthymoquinoneofinigellasativaiinpulptherapyinvitrostudy AT ruaaaalamoudi biologicalpotentialofthemaincomponentthymoquinoneofinigellasativaiinpulptherapyinvitrostudy |