Variations in the fecal microbiota and their functions of Thoroughbred, Mongolian, and Hybrid horses
The horse gut is colonized by a rich and complex microbial community that has important roles in horse physiology, metabolism, nutrition, and immune functions. Fewer across-breed variations in horse gut microbial diversity have been illustrated. In this article, the gut microbiota of Thoroughbred, M...
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| Format: | Article |
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Frontiers Media S.A.
2022-07-01
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| Series: | Frontiers in Veterinary Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2022.920080/full |
| _version_ | 1811311530829938688 |
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| author | Xiaohui Wen Shengjun Luo Dianhong Lv Chunling Jia Xiurong Zhou Qi Zhai Li Xi Caijuan Yang |
| author_facet | Xiaohui Wen Shengjun Luo Dianhong Lv Chunling Jia Xiurong Zhou Qi Zhai Li Xi Caijuan Yang |
| author_sort | Xiaohui Wen |
| collection | DOAJ |
| description | The horse gut is colonized by a rich and complex microbial community that has important roles in horse physiology, metabolism, nutrition, and immune functions. Fewer across-breed variations in horse gut microbial diversity have been illustrated. In this article, the gut microbiota of Thoroughbred, Mongolian, and Hybrid horses [first filial generation (F1) of Mongolian (maternal) and Thoroughbred (paternal)] were studied by second-generation high-throughput sequencing technology. Differences in gut microbiota composition and function between breeds were determined using diversity and functional prediction analysis. The alpha diversity analysis showed that Thoroughbred horses had a more abundant and diverse gut microbiota, while the diversity of gut microbiota in Hybrid horses was intermediate between Thoroughbred and Mongolian horses. Subsequent cluster analysis showed that Hybrid horses have a microbiota composition more similar to Mongolian horses. LEfSe analysis revealed that the bacterial biomarkers for Thoroughbred horses at the family level were Prevotellaceae, Rikenellaceae, Fibrobacteraceae, p_251_o5, Lactobacillaceae, and uncultured_bacterium_o_WCHB1_41; the bacterial biomarker for Mongolian horses was Planococcaceae; and the bacterial biomarkers for Hybrid horses were Moraxellaceae, Enterobacteriaceae, and Ruminococcaceae. The functional prediction results indicated that the metabolic pathways differ significantly between the breeds. Regarding metabolism, the Hybrid horses had the lowest proportion of the carbohydrate metabolic pathways, while the energy metabolic pathway had the highest proportion. The abundance ratios of the remaining eight metabolic pathways in Hybrid horses were between Thoroughbred and Mongolian horses. In conclusion, the results of this study showed an association between horse breeds and gut microbiota. |
| first_indexed | 2024-04-13T10:19:35Z |
| format | Article |
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| issn | 2297-1769 |
| language | English |
| last_indexed | 2024-04-13T10:19:35Z |
| publishDate | 2022-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Veterinary Science |
| spelling | doaj.art-5a27aa04119b40f990ae0c97a615bad42022-12-22T02:50:32ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692022-07-01910.3389/fvets.2022.920080920080Variations in the fecal microbiota and their functions of Thoroughbred, Mongolian, and Hybrid horsesXiaohui Wen0Shengjun Luo1Dianhong Lv2Chunling Jia3Xiurong Zhou4Qi Zhai5Li Xi6Caijuan Yang7Institute of Animal Health, Scientific Observation and Experiment Station of Veterinary Drugs and Diagnostic Techniques of Guangdong Province, Ministry of Agriculture of Rural Affairs, Key Laboratory of Animal Disease Prevention of Guangdong Province, Guangdong Academy of Agricultural Sciences, Guangzhou, ChinaInstitute of Animal Health, Scientific Observation and Experiment Station of Veterinary Drugs and Diagnostic Techniques of Guangdong Province, Ministry of Agriculture of Rural Affairs, Key Laboratory of Animal Disease Prevention of Guangdong Province, Guangdong Academy of Agricultural Sciences, Guangzhou, ChinaInstitute of Animal Health, Scientific Observation and Experiment Station of Veterinary Drugs and Diagnostic Techniques of Guangdong Province, Ministry of Agriculture of Rural Affairs, Key Laboratory of Animal Disease Prevention of Guangdong Province, Guangdong Academy of Agricultural Sciences, Guangzhou, ChinaInstitute of Animal Health, Scientific Observation and Experiment Station of Veterinary Drugs and Diagnostic Techniques of Guangdong Province, Ministry of Agriculture of Rural Affairs, Key Laboratory of Animal Disease Prevention of Guangdong Province, Guangdong Academy of Agricultural Sciences, Guangzhou, ChinaInstitute of Animal Health, Scientific Observation and Experiment Station of Veterinary Drugs and Diagnostic Techniques of Guangdong Province, Ministry of Agriculture of Rural Affairs, Key Laboratory of Animal Disease Prevention of Guangdong Province, Guangdong Academy of Agricultural Sciences, Guangzhou, ChinaInstitute of Animal Health, Scientific Observation and Experiment Station of Veterinary Drugs and Diagnostic Techniques of Guangdong Province, Ministry of Agriculture of Rural Affairs, Key Laboratory of Animal Disease Prevention of Guangdong Province, Guangdong Academy of Agricultural Sciences, Guangzhou, ChinaDepartment of Animal Science, College of Biology and Food, Shangqiu Normal University, Shangqiu, ChinaNational S&T Innovation Center for Modern Agricultural Industry, Guangzhou, ChinaThe horse gut is colonized by a rich and complex microbial community that has important roles in horse physiology, metabolism, nutrition, and immune functions. Fewer across-breed variations in horse gut microbial diversity have been illustrated. In this article, the gut microbiota of Thoroughbred, Mongolian, and Hybrid horses [first filial generation (F1) of Mongolian (maternal) and Thoroughbred (paternal)] were studied by second-generation high-throughput sequencing technology. Differences in gut microbiota composition and function between breeds were determined using diversity and functional prediction analysis. The alpha diversity analysis showed that Thoroughbred horses had a more abundant and diverse gut microbiota, while the diversity of gut microbiota in Hybrid horses was intermediate between Thoroughbred and Mongolian horses. Subsequent cluster analysis showed that Hybrid horses have a microbiota composition more similar to Mongolian horses. LEfSe analysis revealed that the bacterial biomarkers for Thoroughbred horses at the family level were Prevotellaceae, Rikenellaceae, Fibrobacteraceae, p_251_o5, Lactobacillaceae, and uncultured_bacterium_o_WCHB1_41; the bacterial biomarker for Mongolian horses was Planococcaceae; and the bacterial biomarkers for Hybrid horses were Moraxellaceae, Enterobacteriaceae, and Ruminococcaceae. The functional prediction results indicated that the metabolic pathways differ significantly between the breeds. Regarding metabolism, the Hybrid horses had the lowest proportion of the carbohydrate metabolic pathways, while the energy metabolic pathway had the highest proportion. The abundance ratios of the remaining eight metabolic pathways in Hybrid horses were between Thoroughbred and Mongolian horses. In conclusion, the results of this study showed an association between horse breeds and gut microbiota.https://www.frontiersin.org/articles/10.3389/fvets.2022.920080/full16S rRNAbreedmicrobiotadiversityfibrolytic bacteria |
| spellingShingle | Xiaohui Wen Shengjun Luo Dianhong Lv Chunling Jia Xiurong Zhou Qi Zhai Li Xi Caijuan Yang Variations in the fecal microbiota and their functions of Thoroughbred, Mongolian, and Hybrid horses Frontiers in Veterinary Science 16S rRNA breed microbiota diversity fibrolytic bacteria |
| title | Variations in the fecal microbiota and their functions of Thoroughbred, Mongolian, and Hybrid horses |
| title_full | Variations in the fecal microbiota and their functions of Thoroughbred, Mongolian, and Hybrid horses |
| title_fullStr | Variations in the fecal microbiota and their functions of Thoroughbred, Mongolian, and Hybrid horses |
| title_full_unstemmed | Variations in the fecal microbiota and their functions of Thoroughbred, Mongolian, and Hybrid horses |
| title_short | Variations in the fecal microbiota and their functions of Thoroughbred, Mongolian, and Hybrid horses |
| title_sort | variations in the fecal microbiota and their functions of thoroughbred mongolian and hybrid horses |
| topic | 16S rRNA breed microbiota diversity fibrolytic bacteria |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2022.920080/full |
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