Aminophosphate precursors for the synthesis of near‐unity emitting InP quantum dots and their application in liver cancer diagnosis
Abstract InP quantum dots (QDs) are a promising and environment‐friendly alternative to Cd‐based QDs for in vitro diagnostics and bioimaging applications. However, their poor fluorescence and stability severely limit their biological applications. Herein, we synthesize bright (∼100%) and stable InP‐...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2022-08-01
|
| Series: | Exploration |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/EXP.20220082 |
| _version_ | 1828120959209439232 |
|---|---|
| author | Yanbin Zhang Yanbing Lv Lin‐Song Li Xue‐Jie Zhao Mei‐Xia Zhao Huaibin Shen |
| author_facet | Yanbin Zhang Yanbing Lv Lin‐Song Li Xue‐Jie Zhao Mei‐Xia Zhao Huaibin Shen |
| author_sort | Yanbin Zhang |
| collection | DOAJ |
| description | Abstract InP quantum dots (QDs) are a promising and environment‐friendly alternative to Cd‐based QDs for in vitro diagnostics and bioimaging applications. However, their poor fluorescence and stability severely limit their biological applications. Herein, we synthesize bright (∼100%) and stable InP‐based core/shell QDs by using cost‐effective and low‐toxic phosphorus source, and then aqueous InP QDs are prepared with quantum yield over 80% by shell engineering. The immunoassay of alpha‐fetoprotein can be detected in the widest analytical range of 1–1000 ng ml−1 and the limit of detection of 0.58 ng ml−1 by using those InP QDs‐based fluorescent probes, making it the best‐performing heavy metal‐free detection reported so far, comparable to state‐of‐the‐art Cd‐QDs‐based probes. Furthermore, the high‐quality aqueous InP QDs exhibit excellent performance in specific labeling of liver cancer cells and in vivo tumor‐targeted imaging of live mice. Overall, the present work demonstrates the great potential of novel high‐quality Cd‐free InP QDs in cancer diagnosis and image‐guided surgery. |
| first_indexed | 2024-04-11T14:14:32Z |
| format | Article |
| id | doaj.art-5a28b22bc1f943c2865a2e99072b58ad |
| institution | Directory Open Access Journal |
| issn | 2766-8509 2766-2098 |
| language | English |
| last_indexed | 2024-04-11T14:14:32Z |
| publishDate | 2022-08-01 |
| publisher | Wiley |
| record_format | Article |
| series | Exploration |
| spelling | doaj.art-5a28b22bc1f943c2865a2e99072b58ad2022-12-22T04:19:36ZengWileyExploration2766-85092766-20982022-08-0124n/an/a10.1002/EXP.20220082Aminophosphate precursors for the synthesis of near‐unity emitting InP quantum dots and their application in liver cancer diagnosisYanbin Zhang0Yanbing Lv1Lin‐Song Li2Xue‐Jie Zhao3Mei‐Xia Zhao4Huaibin Shen5Key Laboratory of Natural Medicine and Immuno‐Engineering of Henan Province Henan University Kaifeng ChinaKey Laboratory for Special Functional Materials of Ministry of Education School of Materials and Engineering Henan University Kaifeng ChinaKey Laboratory of Natural Medicine and Immuno‐Engineering of Henan Province Henan University Kaifeng ChinaKey Laboratory of Natural Medicine and Immuno‐Engineering of Henan Province Henan University Kaifeng ChinaKey Laboratory of Natural Medicine and Immuno‐Engineering of Henan Province Henan University Kaifeng ChinaKey Laboratory for Special Functional Materials of Ministry of Education School of Materials and Engineering Henan University Kaifeng ChinaAbstract InP quantum dots (QDs) are a promising and environment‐friendly alternative to Cd‐based QDs for in vitro diagnostics and bioimaging applications. However, their poor fluorescence and stability severely limit their biological applications. Herein, we synthesize bright (∼100%) and stable InP‐based core/shell QDs by using cost‐effective and low‐toxic phosphorus source, and then aqueous InP QDs are prepared with quantum yield over 80% by shell engineering. The immunoassay of alpha‐fetoprotein can be detected in the widest analytical range of 1–1000 ng ml−1 and the limit of detection of 0.58 ng ml−1 by using those InP QDs‐based fluorescent probes, making it the best‐performing heavy metal‐free detection reported so far, comparable to state‐of‐the‐art Cd‐QDs‐based probes. Furthermore, the high‐quality aqueous InP QDs exhibit excellent performance in specific labeling of liver cancer cells and in vivo tumor‐targeted imaging of live mice. Overall, the present work demonstrates the great potential of novel high‐quality Cd‐free InP QDs in cancer diagnosis and image‐guided surgery.https://doi.org/10.1002/EXP.20220082bioimagingInP quantum dotsin vitro diagnosticsliver cancershell engineering |
| spellingShingle | Yanbin Zhang Yanbing Lv Lin‐Song Li Xue‐Jie Zhao Mei‐Xia Zhao Huaibin Shen Aminophosphate precursors for the synthesis of near‐unity emitting InP quantum dots and their application in liver cancer diagnosis Exploration bioimaging InP quantum dots in vitro diagnostics liver cancer shell engineering |
| title | Aminophosphate precursors for the synthesis of near‐unity emitting InP quantum dots and their application in liver cancer diagnosis |
| title_full | Aminophosphate precursors for the synthesis of near‐unity emitting InP quantum dots and their application in liver cancer diagnosis |
| title_fullStr | Aminophosphate precursors for the synthesis of near‐unity emitting InP quantum dots and their application in liver cancer diagnosis |
| title_full_unstemmed | Aminophosphate precursors for the synthesis of near‐unity emitting InP quantum dots and their application in liver cancer diagnosis |
| title_short | Aminophosphate precursors for the synthesis of near‐unity emitting InP quantum dots and their application in liver cancer diagnosis |
| title_sort | aminophosphate precursors for the synthesis of near unity emitting inp quantum dots and their application in liver cancer diagnosis |
| topic | bioimaging InP quantum dots in vitro diagnostics liver cancer shell engineering |
| url | https://doi.org/10.1002/EXP.20220082 |
| work_keys_str_mv | AT yanbinzhang aminophosphateprecursorsforthesynthesisofnearunityemittinginpquantumdotsandtheirapplicationinlivercancerdiagnosis AT yanbinglv aminophosphateprecursorsforthesynthesisofnearunityemittinginpquantumdotsandtheirapplicationinlivercancerdiagnosis AT linsongli aminophosphateprecursorsforthesynthesisofnearunityemittinginpquantumdotsandtheirapplicationinlivercancerdiagnosis AT xuejiezhao aminophosphateprecursorsforthesynthesisofnearunityemittinginpquantumdotsandtheirapplicationinlivercancerdiagnosis AT meixiazhao aminophosphateprecursorsforthesynthesisofnearunityemittinginpquantumdotsandtheirapplicationinlivercancerdiagnosis AT huaibinshen aminophosphateprecursorsforthesynthesisofnearunityemittinginpquantumdotsandtheirapplicationinlivercancerdiagnosis |