The Iridoids of In Vitro Propagated <i>Nepeta cyrenaica</i> Quézel & Zaffran
<i>Nepeta cyrenaica</i> Quézel & Zaffran (Lamiaceae), an endemic species of the Libyan flora, has been scarcely explored until now. Since <i>Nepeta</i> species are proven as pharmacologically active plants rich in iridoid monoterpenes, this study was aimed to quantify the...
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MDPI AG
2022-11-01
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| author | Ana Alimpić Aradski Danijela Mišić Uroš Gašić Slađana Todorović Mariana Oalđe Pavlović Petar D. Marin Abdulhamed Giweli Sonja Duletić-Laušević |
| author_facet | Ana Alimpić Aradski Danijela Mišić Uroš Gašić Slađana Todorović Mariana Oalđe Pavlović Petar D. Marin Abdulhamed Giweli Sonja Duletić-Laušević |
| author_sort | Ana Alimpić Aradski |
| collection | DOAJ |
| description | <i>Nepeta cyrenaica</i> Quézel & Zaffran (Lamiaceae), an endemic species of the Libyan flora, has been scarcely explored until now. Since <i>Nepeta</i> species are proven as pharmacologically active plants rich in iridoid monoterpenes, this study was aimed to quantify the iridoids present in different extracts of in vitro propagated <i>N. cyrenaica</i>. The seeds collected from their natural habitat were germinated. One node stem explant of 5-week-old plants were used for plant micropropagation. After several cycles, the shoots were harvested. The extraction was performed using maceration procedure by dichloromehane, methanol, 96% ethanol, and hot distilled water. Subsequently, the extracts were subjected to UHPLC/(±)HESI-MS2 analysis of iridoids. Two iridoids were identified and quantified, namely epideoxyloganic acid and nepetalactol, as well as their precursor 8-oxogeranial. Iridoid glucoside epideoxyloganic acid was the most abundant in the methanolic and ethanolic extracts (1255.37 and 1262.78 µg/100 g dry extract), followed by aqueous extract. On the other hand, 8-oxogeranial and nepetalactol reached maximal amounts in the dichloromethane extract (476.80 and 1039.52 µg/100 g dry extract, respectively). The results of this study indicate that the high antioxidant and enzyme-inhibiting effects of dichloromethane extract confirmed in our previous study could be attributed to its iridoid content, which was particularly high due to the low polarity of this extraction solvent. In conclusion, endemic <i>N. cyrenaica</i> could be efficiently propagated in vitro as an iridoid-rich plant with great biological potential. |
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| publishDate | 2022-11-01 |
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| spelling | doaj.art-5a2ea3c4ec3c4ec6a93ee56fb993167e2023-11-17T12:58:53ZengMDPI AGMedical Sciences Forum2673-99922022-11-0114114710.3390/ECMC2022-13148The Iridoids of In Vitro Propagated <i>Nepeta cyrenaica</i> Quézel & ZaffranAna Alimpić Aradski0Danijela Mišić1Uroš Gašić2Slađana Todorović3Mariana Oalđe Pavlović4Petar D. Marin5Abdulhamed Giweli6Sonja Duletić-Laušević7Institute of Botany and Botanical Garden “Jevremovac”, Faculty of Biology, University of Belgrade, 11000 Belgrade, SerbiaInstitute for Biological Research “Siniša Stanković”—National Institute of the Republic of Serbia, University of Belgrade, 11000 Belgrade, SerbiaInstitute for Biological Research “Siniša Stanković”—National Institute of the Republic of Serbia, University of Belgrade, 11000 Belgrade, SerbiaInstitute for Biological Research “Siniša Stanković”—National Institute of the Republic of Serbia, University of Belgrade, 11000 Belgrade, SerbiaInstitute of Botany and Botanical Garden “Jevremovac”, Faculty of Biology, University of Belgrade, 11000 Belgrade, SerbiaInstitute of Botany and Botanical Garden “Jevremovac”, Faculty of Biology, University of Belgrade, 11000 Belgrade, SerbiaFaculty of Science, University of Al-Zintan, Zintan 501, LibyaInstitute of Botany and Botanical Garden “Jevremovac”, Faculty of Biology, University of Belgrade, 11000 Belgrade, Serbia<i>Nepeta cyrenaica</i> Quézel & Zaffran (Lamiaceae), an endemic species of the Libyan flora, has been scarcely explored until now. Since <i>Nepeta</i> species are proven as pharmacologically active plants rich in iridoid monoterpenes, this study was aimed to quantify the iridoids present in different extracts of in vitro propagated <i>N. cyrenaica</i>. The seeds collected from their natural habitat were germinated. One node stem explant of 5-week-old plants were used for plant micropropagation. After several cycles, the shoots were harvested. The extraction was performed using maceration procedure by dichloromehane, methanol, 96% ethanol, and hot distilled water. Subsequently, the extracts were subjected to UHPLC/(±)HESI-MS2 analysis of iridoids. Two iridoids were identified and quantified, namely epideoxyloganic acid and nepetalactol, as well as their precursor 8-oxogeranial. Iridoid glucoside epideoxyloganic acid was the most abundant in the methanolic and ethanolic extracts (1255.37 and 1262.78 µg/100 g dry extract), followed by aqueous extract. On the other hand, 8-oxogeranial and nepetalactol reached maximal amounts in the dichloromethane extract (476.80 and 1039.52 µg/100 g dry extract, respectively). The results of this study indicate that the high antioxidant and enzyme-inhibiting effects of dichloromethane extract confirmed in our previous study could be attributed to its iridoid content, which was particularly high due to the low polarity of this extraction solvent. In conclusion, endemic <i>N. cyrenaica</i> could be efficiently propagated in vitro as an iridoid-rich plant with great biological potential.https://www.mdpi.com/2673-9992/14/1/147<i>Nepeta cyrenaica</i>in vitro propagarionextractsiridoids |
| spellingShingle | Ana Alimpić Aradski Danijela Mišić Uroš Gašić Slađana Todorović Mariana Oalđe Pavlović Petar D. Marin Abdulhamed Giweli Sonja Duletić-Laušević The Iridoids of In Vitro Propagated <i>Nepeta cyrenaica</i> Quézel & Zaffran Medical Sciences Forum <i>Nepeta cyrenaica</i> in vitro propagarion extracts iridoids |
| title | The Iridoids of In Vitro Propagated <i>Nepeta cyrenaica</i> Quézel & Zaffran |
| title_full | The Iridoids of In Vitro Propagated <i>Nepeta cyrenaica</i> Quézel & Zaffran |
| title_fullStr | The Iridoids of In Vitro Propagated <i>Nepeta cyrenaica</i> Quézel & Zaffran |
| title_full_unstemmed | The Iridoids of In Vitro Propagated <i>Nepeta cyrenaica</i> Quézel & Zaffran |
| title_short | The Iridoids of In Vitro Propagated <i>Nepeta cyrenaica</i> Quézel & Zaffran |
| title_sort | iridoids of in vitro propagated i nepeta cyrenaica i quezel zaffran |
| topic | <i>Nepeta cyrenaica</i> in vitro propagarion extracts iridoids |
| url | https://www.mdpi.com/2673-9992/14/1/147 |
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