Target preference of Type III-A CRISPR-Cas complexes at the transcription bubble
Type III CRISPR-Cas systems are able to target transcriptionally active DNA sequences in phages and plasmids. Here, the authors reveal the mechanism of the target nucleic acid preference of Type III-A CRISPR-Cas complexes at the transcription bubble by a combination of structural and biochemical app...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2019-07-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-019-10780-2 |
| _version_ | 1818835227221426176 |
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| author | Tina Y. Liu Jun-Jie Liu Abhishek J. Aditham Eva Nogales Jennifer A. Doudna |
| author_facet | Tina Y. Liu Jun-Jie Liu Abhishek J. Aditham Eva Nogales Jennifer A. Doudna |
| author_sort | Tina Y. Liu |
| collection | DOAJ |
| description | Type III CRISPR-Cas systems are able to target transcriptionally active DNA sequences in phages and plasmids. Here, the authors reveal the mechanism of the target nucleic acid preference of Type III-A CRISPR-Cas complexes at the transcription bubble by a combination of structural and biochemical approaches. |
| first_indexed | 2024-12-19T02:47:21Z |
| format | Article |
| id | doaj.art-5a2efd0e054746c6934bd0d016c53fc2 |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-12-19T02:47:21Z |
| publishDate | 2019-07-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-5a2efd0e054746c6934bd0d016c53fc22022-12-21T20:38:49ZengNature PortfolioNature Communications2041-17232019-07-0110111310.1038/s41467-019-10780-2Target preference of Type III-A CRISPR-Cas complexes at the transcription bubbleTina Y. Liu0Jun-Jie Liu1Abhishek J. Aditham2Eva Nogales3Jennifer A. Doudna4Department of Molecular and Cell Biology, University of CaliforniaDepartment of Molecular and Cell Biology, University of CaliforniaDepartment of Chemical & Biomolecular Engineering, University of CaliforniaDepartment of Molecular and Cell Biology, University of CaliforniaDepartment of Molecular and Cell Biology, University of CaliforniaType III CRISPR-Cas systems are able to target transcriptionally active DNA sequences in phages and plasmids. Here, the authors reveal the mechanism of the target nucleic acid preference of Type III-A CRISPR-Cas complexes at the transcription bubble by a combination of structural and biochemical approaches.https://doi.org/10.1038/s41467-019-10780-2 |
| spellingShingle | Tina Y. Liu Jun-Jie Liu Abhishek J. Aditham Eva Nogales Jennifer A. Doudna Target preference of Type III-A CRISPR-Cas complexes at the transcription bubble Nature Communications |
| title | Target preference of Type III-A CRISPR-Cas complexes at the transcription bubble |
| title_full | Target preference of Type III-A CRISPR-Cas complexes at the transcription bubble |
| title_fullStr | Target preference of Type III-A CRISPR-Cas complexes at the transcription bubble |
| title_full_unstemmed | Target preference of Type III-A CRISPR-Cas complexes at the transcription bubble |
| title_short | Target preference of Type III-A CRISPR-Cas complexes at the transcription bubble |
| title_sort | target preference of type iii a crispr cas complexes at the transcription bubble |
| url | https://doi.org/10.1038/s41467-019-10780-2 |
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