Recommendations for In Vitro and In Vivo Testing of Magnetic Nanoparticle Hyperthermia Combined with Radiation Therapy
Magnetic nanoparticle (MNP)-mediated hyperthermia (MH) coupled with radiation therapy (RT) is a novel approach that has the potential to overcome various practical difficulties encountered in cancer treatment. In this work, we present recommendations for the in vitro and in vivo testing and applicat...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2018-05-01
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| Series: | Nanomaterials |
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| Online Access: | http://www.mdpi.com/2079-4991/8/5/306 |
| _version_ | 1818245025820049408 |
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| author | Spiridon V. Spirou Sofia A. Costa Lima Penelope Bouziotis Sanja Vranješ-Djurić Eleni Κ. Efthimiadou Anna Laurenzana Ana Isabel Barbosa Ignacio Garcia-Alonso Carlton Jones Drina Jankovic Oliviero L. Gobbo |
| author_facet | Spiridon V. Spirou Sofia A. Costa Lima Penelope Bouziotis Sanja Vranješ-Djurić Eleni Κ. Efthimiadou Anna Laurenzana Ana Isabel Barbosa Ignacio Garcia-Alonso Carlton Jones Drina Jankovic Oliviero L. Gobbo |
| author_sort | Spiridon V. Spirou |
| collection | DOAJ |
| description | Magnetic nanoparticle (MNP)-mediated hyperthermia (MH) coupled with radiation therapy (RT) is a novel approach that has the potential to overcome various practical difficulties encountered in cancer treatment. In this work, we present recommendations for the in vitro and in vivo testing and application of the two treatment techniques. These recommendations were developed by the members of Working Group 3 of COST Action TD 1402: Multifunctional Nanoparticles for Magnetic Hyperthermia and Indirect Radiation Therapy (“Radiomag”). The purpose of the recommendations is not to provide definitive answers and directions but, rather, to outline those tests and considerations that a researcher must address in order to perform in vitro and in vivo studies. The recommendations are divided into 5 parts: (a) in vitro evaluation of MNPs; (b) in vitro evaluation of MNP-cell interactions; (c) in vivo evaluation of the MNPs; (d) MH combined with RT; and (e) pharmacokinetic studies of MNPs. Synthesis and characterization of the MNPs, as well as RT protocols, are beyond the scope of this work. |
| first_indexed | 2024-12-12T14:26:22Z |
| format | Article |
| id | doaj.art-5a303128600641c0998d0a46bd42732f |
| institution | Directory Open Access Journal |
| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-12-12T14:26:22Z |
| publishDate | 2018-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nanomaterials |
| spelling | doaj.art-5a303128600641c0998d0a46bd42732f2022-12-22T00:21:40ZengMDPI AGNanomaterials2079-49912018-05-018530610.3390/nano8050306nano8050306Recommendations for In Vitro and In Vivo Testing of Magnetic Nanoparticle Hyperthermia Combined with Radiation TherapySpiridon V. Spirou0Sofia A. Costa Lima1Penelope Bouziotis2Sanja Vranješ-Djurić3Eleni Κ. Efthimiadou4Anna Laurenzana5Ana Isabel Barbosa6Ignacio Garcia-Alonso7Carlton Jones8Drina Jankovic9Oliviero L. Gobbo10Department of Radiology, Sismanoglio General Hospital of Attica, Sismanogliou 1, Marousi 15126, Athens, GreeceLAQV, REQUIMTE, Departamento de Ciências Químicas, Faculdade de Farmácia, Universidade do Porto, Porto 4050-313, PortugalInstitute of Nuclear & Radiological Sciences & Technology, Energy & Safety, National Center for Scientific Research “Demokritos”, Aghia Paraskevi, Athens 15310, Greece“Vinča” Institute of Nuclear Sciences, University of Belgrade, Belgrade 11351, SerbiaInorganic Chemistry Laboratory, Chemistry Department, National and Kapodistrian University of Athens, Panepistimiopolis, Zografou 15784, GreeceDepartment of Biomedical and Clinical Science “Mario Serio”, University of Florence, 50134 Firenze, ItalyLAQV, REQUIMTE, Departamento de Ciências Químicas, Faculdade de Farmácia, Universidade do Porto, Porto 4050-313, PortugalDepartment of Surgery, Radiology & Ph.M. University of the Basque Country, Bilbao E48940, SpainNanoTherics Ltd., Studio 3, Unit 3, Silverdale Enterprise Centre Kents Lane, Newcastle under Lyme ST5 6SR, UK“Vinča” Institute of Nuclear Sciences, University of Belgrade, Belgrade 11351, SerbiaSchool of Pharmacy and Pharmaceutical Sciences, Panoz Institute, Trinity College Dublin, D02PN40 Dublin, IrelandMagnetic nanoparticle (MNP)-mediated hyperthermia (MH) coupled with radiation therapy (RT) is a novel approach that has the potential to overcome various practical difficulties encountered in cancer treatment. In this work, we present recommendations for the in vitro and in vivo testing and application of the two treatment techniques. These recommendations were developed by the members of Working Group 3 of COST Action TD 1402: Multifunctional Nanoparticles for Magnetic Hyperthermia and Indirect Radiation Therapy (“Radiomag”). The purpose of the recommendations is not to provide definitive answers and directions but, rather, to outline those tests and considerations that a researcher must address in order to perform in vitro and in vivo studies. The recommendations are divided into 5 parts: (a) in vitro evaluation of MNPs; (b) in vitro evaluation of MNP-cell interactions; (c) in vivo evaluation of the MNPs; (d) MH combined with RT; and (e) pharmacokinetic studies of MNPs. Synthesis and characterization of the MNPs, as well as RT protocols, are beyond the scope of this work.http://www.mdpi.com/2079-4991/8/5/306animal modelsbiodistributionin vitro assaysin vivo evaluationmagnetic nanoparticlespharmacokinetics |
| spellingShingle | Spiridon V. Spirou Sofia A. Costa Lima Penelope Bouziotis Sanja Vranješ-Djurić Eleni Κ. Efthimiadou Anna Laurenzana Ana Isabel Barbosa Ignacio Garcia-Alonso Carlton Jones Drina Jankovic Oliviero L. Gobbo Recommendations for In Vitro and In Vivo Testing of Magnetic Nanoparticle Hyperthermia Combined with Radiation Therapy Nanomaterials animal models biodistribution in vitro assays in vivo evaluation magnetic nanoparticles pharmacokinetics |
| title | Recommendations for In Vitro and In Vivo Testing of Magnetic Nanoparticle Hyperthermia Combined with Radiation Therapy |
| title_full | Recommendations for In Vitro and In Vivo Testing of Magnetic Nanoparticle Hyperthermia Combined with Radiation Therapy |
| title_fullStr | Recommendations for In Vitro and In Vivo Testing of Magnetic Nanoparticle Hyperthermia Combined with Radiation Therapy |
| title_full_unstemmed | Recommendations for In Vitro and In Vivo Testing of Magnetic Nanoparticle Hyperthermia Combined with Radiation Therapy |
| title_short | Recommendations for In Vitro and In Vivo Testing of Magnetic Nanoparticle Hyperthermia Combined with Radiation Therapy |
| title_sort | recommendations for in vitro and in vivo testing of magnetic nanoparticle hyperthermia combined with radiation therapy |
| topic | animal models biodistribution in vitro assays in vivo evaluation magnetic nanoparticles pharmacokinetics |
| url | http://www.mdpi.com/2079-4991/8/5/306 |
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