Evidence for the Involvement of Spinal Cord-Inhibitory and Cytokines-Modulatory Mechanisms in the Anti-Hyperalgesic Effect of Hecogenin Acetate, a Steroidal Sapogenin-Acetylated, in Mice
Hecogenin is a steroidal sapogenin largely drawn from the plants of the genus Agave, commonly known as ‘sisal’, and is one of the important precursors used by the pharmaceutical industry for the synthesis of steroid hormones. Hecogenin acetate (HA) is a steroidal sapogenin-acetylated that produces a...
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| Format: | Article |
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MDPI AG
2014-06-01
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| Series: | Molecules |
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| Online Access: | http://www.mdpi.com/1420-3049/19/6/8303 |
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| author | Jullyana S.S. Quintans Rosana S.S. Barreto Waldecy de Lucca Cristiane F. Villarreal Carla M. Kaneto Milena B.P. Soares Alexsandro Branco Jackson R.G.S. Almeida Alex G. Taranto Angelo R. Antoniolli Rivelilson M. Freitas Lucindo J. Quintans-Júnior |
| author_facet | Jullyana S.S. Quintans Rosana S.S. Barreto Waldecy de Lucca Cristiane F. Villarreal Carla M. Kaneto Milena B.P. Soares Alexsandro Branco Jackson R.G.S. Almeida Alex G. Taranto Angelo R. Antoniolli Rivelilson M. Freitas Lucindo J. Quintans-Júnior |
| author_sort | Jullyana S.S. Quintans |
| collection | DOAJ |
| description | Hecogenin is a steroidal sapogenin largely drawn from the plants of the genus Agave, commonly known as ‘sisal’, and is one of the important precursors used by the pharmaceutical industry for the synthesis of steroid hormones. Hecogenin acetate (HA) is a steroidal sapogenin-acetylated that produces antinociceptive activity. Thus, we evaluate the antihyperalgesic profile of HA in mice in inflammatory models, as well as its possible involvement with c-fos expression on spinal cord area and cytokines to produces analgesic profile. Acute pretreatment with HA (5, 10, or 20 mg/kg; i.p.) inhibited the development of mechanical hyperalgesia induced by carrageenan, TNF-α, dopamine and PGE2. Additionally, the immunofluorescence data demonstrated that acute pretreatment with HA, at all doses tested, significantly inhibited Fos-like expression in the spinal cord dorsal horn normally observed after carrageenan-inflammation. Moreover, HA did not affect the motor performance of the mice as tested in the Rota rod test. This antinociceptive profile seems to be related, at least in part, to a reduction of pro-inflammatory cytokines, as IL-1β. The present results suggest that HA attenuates mechanical hyperalgesia by blocking the neural transmission of pain at the spinal cord levels and by cytokines-inhibitory mechanisms. |
| first_indexed | 2024-12-21T15:33:16Z |
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| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-21T15:33:16Z |
| publishDate | 2014-06-01 |
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| record_format | Article |
| series | Molecules |
| spelling | doaj.art-5a320f295bf84f52a8527bf3ecf809cb2022-12-21T18:58:42ZengMDPI AGMolecules1420-30492014-06-011968303831610.3390/molecules19068303molecules19068303Evidence for the Involvement of Spinal Cord-Inhibitory and Cytokines-Modulatory Mechanisms in the Anti-Hyperalgesic Effect of Hecogenin Acetate, a Steroidal Sapogenin-Acetylated, in MiceJullyana S.S. Quintans0Rosana S.S. Barreto1Waldecy de Lucca2Cristiane F. Villarreal3Carla M. Kaneto4Milena B.P. Soares5Alexsandro Branco6Jackson R.G.S. Almeida7Alex G. Taranto8Angelo R. Antoniolli9Rivelilson M. Freitas10Lucindo J. Quintans-Júnior11Departamento de Fisiologia. Universidade Federal de Sergipe-UFS, Av. Marechal Rondom, s/n, São Cristóvão, Sergipe, CEP 49.000-100, BrazilDepartamento de Fisiologia. Universidade Federal de Sergipe-UFS, Av. Marechal Rondom, s/n, São Cristóvão, Sergipe, CEP 49.000-100, BrazilDepartamento de Fisiologia. Universidade Federal de Sergipe-UFS, Av. Marechal Rondom, s/n, São Cristóvão, Sergipe, CEP 49.000-100, BrazilFaculdade de Farmácia, Universidade Federal da Bahia, Salvador, Bahia, CEP 40.170-115, BrazilCentro de Biotecnologia e Terapia Celular, Hospital São Rafael, Salvador, Bahia, CEP 41.253-190, BrazilCentro de Biotecnologia e Terapia Celular, Hospital São Rafael, Salvador, Bahia, CEP 41.253-190, BrazilColegiado de Ciências Farmacêuticas, Universidade Estadual de Feira de Santana, Feira de Santana, Bahia, CEP 44.036-900, BrazilColegiado de Ciências Farmacêuticas. Universidade Federal do Vale do São Francisco, Petrolina, CEP 56.304-205, Pernambuco, BrazilCurso de Farmácia, Universidade Federal de São João Del-Rei, Campus Centro Oeste Dona Lindu, Divinópolis, Minas Gerais, CEP 35.501-296, BrazilDepartamento de Fisiologia. Universidade Federal de Sergipe-UFS, Av. Marechal Rondom, s/n, São Cristóvão, Sergipe, CEP 49.000-100, BrazilDepartamento de Farmácia, Universidade Federal do Piauí, Teresina, Piauí, CEP 64.049-550, BrazilDepartamento de Fisiologia. Universidade Federal de Sergipe-UFS, Av. Marechal Rondom, s/n, São Cristóvão, Sergipe, CEP 49.000-100, BrazilHecogenin is a steroidal sapogenin largely drawn from the plants of the genus Agave, commonly known as ‘sisal’, and is one of the important precursors used by the pharmaceutical industry for the synthesis of steroid hormones. Hecogenin acetate (HA) is a steroidal sapogenin-acetylated that produces antinociceptive activity. Thus, we evaluate the antihyperalgesic profile of HA in mice in inflammatory models, as well as its possible involvement with c-fos expression on spinal cord area and cytokines to produces analgesic profile. Acute pretreatment with HA (5, 10, or 20 mg/kg; i.p.) inhibited the development of mechanical hyperalgesia induced by carrageenan, TNF-α, dopamine and PGE2. Additionally, the immunofluorescence data demonstrated that acute pretreatment with HA, at all doses tested, significantly inhibited Fos-like expression in the spinal cord dorsal horn normally observed after carrageenan-inflammation. Moreover, HA did not affect the motor performance of the mice as tested in the Rota rod test. This antinociceptive profile seems to be related, at least in part, to a reduction of pro-inflammatory cytokines, as IL-1β. The present results suggest that HA attenuates mechanical hyperalgesia by blocking the neural transmission of pain at the spinal cord levels and by cytokines-inhibitory mechanisms.http://www.mdpi.com/1420-3049/19/6/8303hecogenin acetatesteroidal sapogeninspinal cordc-fosIL-1βpain |
| spellingShingle | Jullyana S.S. Quintans Rosana S.S. Barreto Waldecy de Lucca Cristiane F. Villarreal Carla M. Kaneto Milena B.P. Soares Alexsandro Branco Jackson R.G.S. Almeida Alex G. Taranto Angelo R. Antoniolli Rivelilson M. Freitas Lucindo J. Quintans-Júnior Evidence for the Involvement of Spinal Cord-Inhibitory and Cytokines-Modulatory Mechanisms in the Anti-Hyperalgesic Effect of Hecogenin Acetate, a Steroidal Sapogenin-Acetylated, in Mice Molecules hecogenin acetate steroidal sapogenin spinal cord c-fos IL-1β pain |
| title | Evidence for the Involvement of Spinal Cord-Inhibitory and Cytokines-Modulatory Mechanisms in the Anti-Hyperalgesic Effect of Hecogenin Acetate, a Steroidal Sapogenin-Acetylated, in Mice |
| title_full | Evidence for the Involvement of Spinal Cord-Inhibitory and Cytokines-Modulatory Mechanisms in the Anti-Hyperalgesic Effect of Hecogenin Acetate, a Steroidal Sapogenin-Acetylated, in Mice |
| title_fullStr | Evidence for the Involvement of Spinal Cord-Inhibitory and Cytokines-Modulatory Mechanisms in the Anti-Hyperalgesic Effect of Hecogenin Acetate, a Steroidal Sapogenin-Acetylated, in Mice |
| title_full_unstemmed | Evidence for the Involvement of Spinal Cord-Inhibitory and Cytokines-Modulatory Mechanisms in the Anti-Hyperalgesic Effect of Hecogenin Acetate, a Steroidal Sapogenin-Acetylated, in Mice |
| title_short | Evidence for the Involvement of Spinal Cord-Inhibitory and Cytokines-Modulatory Mechanisms in the Anti-Hyperalgesic Effect of Hecogenin Acetate, a Steroidal Sapogenin-Acetylated, in Mice |
| title_sort | evidence for the involvement of spinal cord inhibitory and cytokines modulatory mechanisms in the anti hyperalgesic effect of hecogenin acetate a steroidal sapogenin acetylated in mice |
| topic | hecogenin acetate steroidal sapogenin spinal cord c-fos IL-1β pain |
| url | http://www.mdpi.com/1420-3049/19/6/8303 |
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