Nanofiber‐based glaucoma drainage implant improves surgical outcomes by modulating fibroblast behavior
Abstract Biomaterials are implanted in millions of individuals worldwide each year. Both naturally derived and synthetic biomaterials induce a foreign body reaction that often culminates in fibrotic encapsulation and reduced functional lifespan. In ophthalmology, glaucoma drainage implants (GDIs) ar...
| Main Authors: | , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2023-05-01
|
| Series: | Bioengineering & Translational Medicine |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/btm2.10487 |
| _version_ | 1797825381284184064 |
|---|---|
| author | Aditya Josyula Ann Mozzer Julia Szeto Youlim Ha Nicole Richmond Seung Woo Chung Sri Vishnu Kiran Rompicharla Janani Narayan Samiksha Ramesh Justin Hanes Laura Ensign Kunal Parikh Ian Pitha |
| author_facet | Aditya Josyula Ann Mozzer Julia Szeto Youlim Ha Nicole Richmond Seung Woo Chung Sri Vishnu Kiran Rompicharla Janani Narayan Samiksha Ramesh Justin Hanes Laura Ensign Kunal Parikh Ian Pitha |
| author_sort | Aditya Josyula |
| collection | DOAJ |
| description | Abstract Biomaterials are implanted in millions of individuals worldwide each year. Both naturally derived and synthetic biomaterials induce a foreign body reaction that often culminates in fibrotic encapsulation and reduced functional lifespan. In ophthalmology, glaucoma drainage implants (GDIs) are implanted in the eye to reduce intraocular pressure (IOP) in order to prevent glaucoma progression and vision loss. Despite recent efforts towards miniaturization and surface chemistry modification, clinically available GDIs are susceptible to high rates of fibrosis and surgical failure. Here, we describe the development of synthetic, nanofiber‐based GDIs with partially degradable inner cores. We evaluated GDIs with nanofiber or smooth surfaces to investigate the effect of surface topography on implant performance. We observed in vitro that nanofiber surfaces supported fibroblast integration and quiescence, even in the presence of pro‐fibrotic signals, compared to smooth surfaces. In rabbit eyes, GDIs with a nanofiber architecture were biocompatible, prevented hypotony, and provided a volumetric aqueous outflow comparable to commercially available GDIs, though with significantly reduced fibrotic encapsulation and expression of key fibrotic markers in the surrounding tissue. We propose that the physical cues provided by the surface of the nanofiber‐based GDIs mimic healthy extracellular matrix structure, mitigating fibroblast activation and potentially extending functional GDI lifespan. |
| first_indexed | 2024-03-13T10:53:17Z |
| format | Article |
| id | doaj.art-5a328a859a0b4ff6842687b015f31c2e |
| institution | Directory Open Access Journal |
| issn | 2380-6761 |
| language | English |
| last_indexed | 2024-03-13T10:53:17Z |
| publishDate | 2023-05-01 |
| publisher | Wiley |
| record_format | Article |
| series | Bioengineering & Translational Medicine |
| spelling | doaj.art-5a328a859a0b4ff6842687b015f31c2e2023-05-17T07:33:11ZengWileyBioengineering & Translational Medicine2380-67612023-05-0183n/an/a10.1002/btm2.10487Nanofiber‐based glaucoma drainage implant improves surgical outcomes by modulating fibroblast behaviorAditya Josyula0Ann Mozzer1Julia Szeto2Youlim Ha3Nicole Richmond4Seung Woo Chung5Sri Vishnu Kiran Rompicharla6Janani Narayan7Samiksha Ramesh8Justin Hanes9Laura Ensign10Kunal Parikh11Ian Pitha12Center for Nanomedicine Johns Hopkins University School of Medicine Baltimore Maryland USACenter for Nanomedicine Johns Hopkins University School of Medicine Baltimore Maryland USACenter for Nanomedicine Johns Hopkins University School of Medicine Baltimore Maryland USACenter for Nanomedicine Johns Hopkins University School of Medicine Baltimore Maryland USACenter for Nanomedicine Johns Hopkins University School of Medicine Baltimore Maryland USACenter for Nanomedicine Johns Hopkins University School of Medicine Baltimore Maryland USACenter for Nanomedicine Johns Hopkins University School of Medicine Baltimore Maryland USACenter for Nanomedicine Johns Hopkins University School of Medicine Baltimore Maryland USACenter for Nanomedicine Johns Hopkins University School of Medicine Baltimore Maryland USACenter for Nanomedicine Johns Hopkins University School of Medicine Baltimore Maryland USACenter for Nanomedicine Johns Hopkins University School of Medicine Baltimore Maryland USACenter for Nanomedicine Johns Hopkins University School of Medicine Baltimore Maryland USACenter for Nanomedicine Johns Hopkins University School of Medicine Baltimore Maryland USAAbstract Biomaterials are implanted in millions of individuals worldwide each year. Both naturally derived and synthetic biomaterials induce a foreign body reaction that often culminates in fibrotic encapsulation and reduced functional lifespan. In ophthalmology, glaucoma drainage implants (GDIs) are implanted in the eye to reduce intraocular pressure (IOP) in order to prevent glaucoma progression and vision loss. Despite recent efforts towards miniaturization and surface chemistry modification, clinically available GDIs are susceptible to high rates of fibrosis and surgical failure. Here, we describe the development of synthetic, nanofiber‐based GDIs with partially degradable inner cores. We evaluated GDIs with nanofiber or smooth surfaces to investigate the effect of surface topography on implant performance. We observed in vitro that nanofiber surfaces supported fibroblast integration and quiescence, even in the presence of pro‐fibrotic signals, compared to smooth surfaces. In rabbit eyes, GDIs with a nanofiber architecture were biocompatible, prevented hypotony, and provided a volumetric aqueous outflow comparable to commercially available GDIs, though with significantly reduced fibrotic encapsulation and expression of key fibrotic markers in the surrounding tissue. We propose that the physical cues provided by the surface of the nanofiber‐based GDIs mimic healthy extracellular matrix structure, mitigating fibroblast activation and potentially extending functional GDI lifespan.https://doi.org/10.1002/btm2.10487fibrosisglaucoma shuntsnanofibersocular biomaterials |
| spellingShingle | Aditya Josyula Ann Mozzer Julia Szeto Youlim Ha Nicole Richmond Seung Woo Chung Sri Vishnu Kiran Rompicharla Janani Narayan Samiksha Ramesh Justin Hanes Laura Ensign Kunal Parikh Ian Pitha Nanofiber‐based glaucoma drainage implant improves surgical outcomes by modulating fibroblast behavior Bioengineering & Translational Medicine fibrosis glaucoma shunts nanofibers ocular biomaterials |
| title | Nanofiber‐based glaucoma drainage implant improves surgical outcomes by modulating fibroblast behavior |
| title_full | Nanofiber‐based glaucoma drainage implant improves surgical outcomes by modulating fibroblast behavior |
| title_fullStr | Nanofiber‐based glaucoma drainage implant improves surgical outcomes by modulating fibroblast behavior |
| title_full_unstemmed | Nanofiber‐based glaucoma drainage implant improves surgical outcomes by modulating fibroblast behavior |
| title_short | Nanofiber‐based glaucoma drainage implant improves surgical outcomes by modulating fibroblast behavior |
| title_sort | nanofiber based glaucoma drainage implant improves surgical outcomes by modulating fibroblast behavior |
| topic | fibrosis glaucoma shunts nanofibers ocular biomaterials |
| url | https://doi.org/10.1002/btm2.10487 |
| work_keys_str_mv | AT adityajosyula nanofiberbasedglaucomadrainageimplantimprovessurgicaloutcomesbymodulatingfibroblastbehavior AT annmozzer nanofiberbasedglaucomadrainageimplantimprovessurgicaloutcomesbymodulatingfibroblastbehavior AT juliaszeto nanofiberbasedglaucomadrainageimplantimprovessurgicaloutcomesbymodulatingfibroblastbehavior AT youlimha nanofiberbasedglaucomadrainageimplantimprovessurgicaloutcomesbymodulatingfibroblastbehavior AT nicolerichmond nanofiberbasedglaucomadrainageimplantimprovessurgicaloutcomesbymodulatingfibroblastbehavior AT seungwoochung nanofiberbasedglaucomadrainageimplantimprovessurgicaloutcomesbymodulatingfibroblastbehavior AT srivishnukiranrompicharla nanofiberbasedglaucomadrainageimplantimprovessurgicaloutcomesbymodulatingfibroblastbehavior AT jananinarayan nanofiberbasedglaucomadrainageimplantimprovessurgicaloutcomesbymodulatingfibroblastbehavior AT samiksharamesh nanofiberbasedglaucomadrainageimplantimprovessurgicaloutcomesbymodulatingfibroblastbehavior AT justinhanes nanofiberbasedglaucomadrainageimplantimprovessurgicaloutcomesbymodulatingfibroblastbehavior AT lauraensign nanofiberbasedglaucomadrainageimplantimprovessurgicaloutcomesbymodulatingfibroblastbehavior AT kunalparikh nanofiberbasedglaucomadrainageimplantimprovessurgicaloutcomesbymodulatingfibroblastbehavior AT ianpitha nanofiberbasedglaucomadrainageimplantimprovessurgicaloutcomesbymodulatingfibroblastbehavior |