Genotoxicity and mutagenicity of water contaminated with tannery effluents, as evaluated by the micronucleus test and comet assay using the fish Oreochromis niloticus and chromosome aberrations in onion root-tips
Cytotoxicity of metals is important because some metals are potential mutagens able to induce tumors in humans and experimental animals. Chromium can damage DNA in several ways, including DNA double strand breaks (DSBs) which generate chromosomal aberrations, micronucleus formation, sister chromatid...
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Genética
2006-01-01
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| Series: | Genetics and Molecular Biology |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000100028 |
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| author | Silvia Tamie Matsumoto Mário Sérgio Mantovani Mirtis Irene Ariza Malaguttii Ana Lúcia Dias Inês Cristina Fonseca Maria Aparecida Marin-Morales |
| author_facet | Silvia Tamie Matsumoto Mário Sérgio Mantovani Mirtis Irene Ariza Malaguttii Ana Lúcia Dias Inês Cristina Fonseca Maria Aparecida Marin-Morales |
| author_sort | Silvia Tamie Matsumoto |
| collection | DOAJ |
| description | Cytotoxicity of metals is important because some metals are potential mutagens able to induce tumors in humans and experimental animals. Chromium can damage DNA in several ways, including DNA double strand breaks (DSBs) which generate chromosomal aberrations, micronucleus formation, sister chromatid exchange, formation of DNA adducts and alterations in DNA replication and transcription. In our study, water samples from three sites in the Córrego dos Bagres stream in the Franca municipality of the Brazilian state of São Paulo were subjected to the comet assay and micronucleus test using erythrocytes from the fish Oreochromis niloticus. Nuclear abnormalities of the erythrocytes included blebbed, notched and lobed nuclei, probably due to genotoxic chromium compounds. The greatest comet assay damage occurred with water from a chromium-containing tannery effluent discharge site, supporting the hypothesis that chromium residues can be genotoxic. The mutagenicity of the water samples was assessed using the onion root-tip cell assay, the most frequent chromosomal abnormalities observed being: c-metaphases, stick chromosome, chromosome breaks and losses, bridged anaphases, multipolar anaphases, and micronucleated and binucleated cells. Onion root-tip cell mutagenicity was highest for water samples containing the highest levels of chromium. |
| first_indexed | 2024-12-15T00:32:58Z |
| format | Article |
| id | doaj.art-5a3601d5acdb4d5e87933a1b560e6eb6 |
| institution | Directory Open Access Journal |
| issn | 1415-4757 1678-4685 |
| language | English |
| last_indexed | 2024-12-15T00:32:58Z |
| publishDate | 2006-01-01 |
| publisher | Sociedade Brasileira de Genética |
| record_format | Article |
| series | Genetics and Molecular Biology |
| spelling | doaj.art-5a3601d5acdb4d5e87933a1b560e6eb62022-12-21T22:41:56ZengSociedade Brasileira de GenéticaGenetics and Molecular Biology1415-47571678-46852006-01-0129114815810.1590/S1415-47572006000100028Genotoxicity and mutagenicity of water contaminated with tannery effluents, as evaluated by the micronucleus test and comet assay using the fish Oreochromis niloticus and chromosome aberrations in onion root-tipsSilvia Tamie MatsumotoMário Sérgio MantovaniMirtis Irene Ariza MalaguttiiAna Lúcia DiasInês Cristina FonsecaMaria Aparecida Marin-MoralesCytotoxicity of metals is important because some metals are potential mutagens able to induce tumors in humans and experimental animals. Chromium can damage DNA in several ways, including DNA double strand breaks (DSBs) which generate chromosomal aberrations, micronucleus formation, sister chromatid exchange, formation of DNA adducts and alterations in DNA replication and transcription. In our study, water samples from three sites in the Córrego dos Bagres stream in the Franca municipality of the Brazilian state of São Paulo were subjected to the comet assay and micronucleus test using erythrocytes from the fish Oreochromis niloticus. Nuclear abnormalities of the erythrocytes included blebbed, notched and lobed nuclei, probably due to genotoxic chromium compounds. The greatest comet assay damage occurred with water from a chromium-containing tannery effluent discharge site, supporting the hypothesis that chromium residues can be genotoxic. The mutagenicity of the water samples was assessed using the onion root-tip cell assay, the most frequent chromosomal abnormalities observed being: c-metaphases, stick chromosome, chromosome breaks and losses, bridged anaphases, multipolar anaphases, and micronucleated and binucleated cells. Onion root-tip cell mutagenicity was highest for water samples containing the highest levels of chromium.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000100028Allium cepachromiumchromosomes aberrationscomet assaymicronucleusOreochromis niloticus |
| spellingShingle | Silvia Tamie Matsumoto Mário Sérgio Mantovani Mirtis Irene Ariza Malaguttii Ana Lúcia Dias Inês Cristina Fonseca Maria Aparecida Marin-Morales Genotoxicity and mutagenicity of water contaminated with tannery effluents, as evaluated by the micronucleus test and comet assay using the fish Oreochromis niloticus and chromosome aberrations in onion root-tips Genetics and Molecular Biology Allium cepa chromium chromosomes aberrations comet assay micronucleus Oreochromis niloticus |
| title | Genotoxicity and mutagenicity of water contaminated with tannery effluents, as evaluated by the micronucleus test and comet assay using the fish Oreochromis niloticus and chromosome aberrations in onion root-tips |
| title_full | Genotoxicity and mutagenicity of water contaminated with tannery effluents, as evaluated by the micronucleus test and comet assay using the fish Oreochromis niloticus and chromosome aberrations in onion root-tips |
| title_fullStr | Genotoxicity and mutagenicity of water contaminated with tannery effluents, as evaluated by the micronucleus test and comet assay using the fish Oreochromis niloticus and chromosome aberrations in onion root-tips |
| title_full_unstemmed | Genotoxicity and mutagenicity of water contaminated with tannery effluents, as evaluated by the micronucleus test and comet assay using the fish Oreochromis niloticus and chromosome aberrations in onion root-tips |
| title_short | Genotoxicity and mutagenicity of water contaminated with tannery effluents, as evaluated by the micronucleus test and comet assay using the fish Oreochromis niloticus and chromosome aberrations in onion root-tips |
| title_sort | genotoxicity and mutagenicity of water contaminated with tannery effluents as evaluated by the micronucleus test and comet assay using the fish oreochromis niloticus and chromosome aberrations in onion root tips |
| topic | Allium cepa chromium chromosomes aberrations comet assay micronucleus Oreochromis niloticus |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000100028 |
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