Cultivation-independent comprehensive survey of bacterial diversity in Tulsi Shyam Hot Springs, India
A taxonomic description of bacteria was deduced from 5.78 Mb metagenomic sequence retrieved from Tulsi Shyam hot spring, India using bacterial tag-encoded FLX amplicon pyrosequencing (bTEFAP). Metagenome contained 10,893 16S rDNA sequences that were analyzed by MG-RAST server to generate the compreh...
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| Format: | Article |
| Language: | English |
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Elsevier
2015-06-01
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| Series: | Genomics Data |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213596015000203 |
| _version_ | 1818217712816488448 |
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| author | Anjana Ghelani Rajesh Patel Amitsinh Mangrola Pravin Dudhagara |
| author_facet | Anjana Ghelani Rajesh Patel Amitsinh Mangrola Pravin Dudhagara |
| author_sort | Anjana Ghelani |
| collection | DOAJ |
| description | A taxonomic description of bacteria was deduced from 5.78 Mb metagenomic sequence retrieved from Tulsi Shyam hot spring, India using bacterial tag-encoded FLX amplicon pyrosequencing (bTEFAP). Metagenome contained 10,893 16S rDNA sequences that were analyzed by MG-RAST server to generate the comprehensive profile of bacteria. Metagenomic data are available at EBI under EBI Metagenomics database with accession no. ERP009559. Metagenome sequences represented the 98.2% bacteria origin, 1.5% of eukaryotic and 0.3% were unidentified. A total of 16 bacterial phyla demonstrating 97 families and 287 species were revealed in the hot spring metagenome. Most abundant phyla were Firmicutes (65.38%), Proteobacteria (21.21%) and unclassified bacteria (10.69%). Whereas, Peptostreptococcaceae (37.33%), Clostridiaceae (23.36%), and Enterobacteriaceae (16.37%) were highest reported families in metagenome. Ubiquitous species were Clostridium bifermentans (17.47%), Clostridium lituseburense (13.93%) and uncultured bacterium (10.15%). Our data provide new information on hot spring bacteria and shed light on their abundance, diversity, distribution and coexisting organisms. |
| first_indexed | 2024-12-12T07:12:14Z |
| format | Article |
| id | doaj.art-5a37f06d208d4e98b7ac7e7dff3b6f97 |
| institution | Directory Open Access Journal |
| issn | 2213-5960 |
| language | English |
| last_indexed | 2024-12-12T07:12:14Z |
| publishDate | 2015-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Genomics Data |
| spelling | doaj.art-5a37f06d208d4e98b7ac7e7dff3b6f972022-12-22T00:33:35ZengElsevierGenomics Data2213-59602015-06-014C545610.1016/j.gdata.2015.03.003Cultivation-independent comprehensive survey of bacterial diversity in Tulsi Shyam Hot Springs, IndiaAnjana Ghelani0Rajesh Patel1Amitsinh Mangrola2Pravin Dudhagara3Department of Microbiology, Shree Ramkrishna Institute of Computer Education and Applied Sciences, Surat, IndiaDepartment of Life Sciences, Hemchandracharya North Gujarat University, Patan, IndiaDepartment of Life Sciences, Hemchandracharya North Gujarat University, Patan, IndiaDepartment of Biotechnology, Veer Narmad South Gujarat University, Surat, IndiaA taxonomic description of bacteria was deduced from 5.78 Mb metagenomic sequence retrieved from Tulsi Shyam hot spring, India using bacterial tag-encoded FLX amplicon pyrosequencing (bTEFAP). Metagenome contained 10,893 16S rDNA sequences that were analyzed by MG-RAST server to generate the comprehensive profile of bacteria. Metagenomic data are available at EBI under EBI Metagenomics database with accession no. ERP009559. Metagenome sequences represented the 98.2% bacteria origin, 1.5% of eukaryotic and 0.3% were unidentified. A total of 16 bacterial phyla demonstrating 97 families and 287 species were revealed in the hot spring metagenome. Most abundant phyla were Firmicutes (65.38%), Proteobacteria (21.21%) and unclassified bacteria (10.69%). Whereas, Peptostreptococcaceae (37.33%), Clostridiaceae (23.36%), and Enterobacteriaceae (16.37%) were highest reported families in metagenome. Ubiquitous species were Clostridium bifermentans (17.47%), Clostridium lituseburense (13.93%) and uncultured bacterium (10.15%). Our data provide new information on hot spring bacteria and shed light on their abundance, diversity, distribution and coexisting organisms.http://www.sciencedirect.com/science/article/pii/S2213596015000203Bacterial diversityMetagenomeTulsi ShyamHot springbTEFAPCultivation-independent |
| spellingShingle | Anjana Ghelani Rajesh Patel Amitsinh Mangrola Pravin Dudhagara Cultivation-independent comprehensive survey of bacterial diversity in Tulsi Shyam Hot Springs, India Genomics Data Bacterial diversity Metagenome Tulsi Shyam Hot spring bTEFAP Cultivation-independent |
| title | Cultivation-independent comprehensive survey of bacterial diversity in Tulsi Shyam Hot Springs, India |
| title_full | Cultivation-independent comprehensive survey of bacterial diversity in Tulsi Shyam Hot Springs, India |
| title_fullStr | Cultivation-independent comprehensive survey of bacterial diversity in Tulsi Shyam Hot Springs, India |
| title_full_unstemmed | Cultivation-independent comprehensive survey of bacterial diversity in Tulsi Shyam Hot Springs, India |
| title_short | Cultivation-independent comprehensive survey of bacterial diversity in Tulsi Shyam Hot Springs, India |
| title_sort | cultivation independent comprehensive survey of bacterial diversity in tulsi shyam hot springs india |
| topic | Bacterial diversity Metagenome Tulsi Shyam Hot spring bTEFAP Cultivation-independent |
| url | http://www.sciencedirect.com/science/article/pii/S2213596015000203 |
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