Study of Silymarin and Vitamin E Protective Effects on Silver Nanoparticle Toxicity on Mice Liver Primary Cell Culture
Nanotechnology is a most promising field for generating new applications in medicine, although, only few nano products are currently in use for medical purposes. A most prominent nanoproduct is nanosilver. Nano-silver has biological properties which are significant for consumer products, food techno...
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2016-03-01
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| Series: | Acta Medica Iranica |
| Subjects: | |
| Online Access: | https://acta.tums.ac.ir/index.php/acta/article/view/5493 |
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| author | Firouz Faedmaleki Farshad H Shirazi Shahram Ejtemaeimehr Soghra Anjarani Amir-Ahmad Salarian Hamidreza Ahmadi Ashtiani Hossein Rastegar |
| author_facet | Firouz Faedmaleki Farshad H Shirazi Shahram Ejtemaeimehr Soghra Anjarani Amir-Ahmad Salarian Hamidreza Ahmadi Ashtiani Hossein Rastegar |
| author_sort | Firouz Faedmaleki |
| collection | DOAJ |
| description | Nanotechnology is a most promising field for generating new applications in medicine, although, only few nano products are currently in use for medical purposes. A most prominent nanoproduct is nanosilver. Nano-silver has biological properties which are significant for consumer products, food technology, textiles, and medical applications (e.g. wound care products, implantable medical devices, in diagnosis, drug delivery, and imaging). For their antibacterial activity, silver nanoparticles (Ag NPs) are largely used in various commercially available products. The use of nano-silver is becoming more and more widespread in medicine and related applications, and due to its increasing exposure, toxicological and environmental issues need to be raised. Cytotoxicity induced by silver nanoparticles (AgNPs) and the role that oxidative stress plays in this process were demonstrated in human hepatoma cells AgNPs agglomerated in the cytoplasm and nuclei of treated cells, and they induced intracellular oxidative stress. AgNP reduced ATP content of the cell and caused damage to mitochondria and increased production of reactive oxygen species (ROS) in a dose-dependent manner. Silymarin was known as a hepatoprotective agent that is used in the treatment of hepatic diseases including viral hepatitis, alcoholic liver diseases, Amanita mushroom poisoning, liver cirrhosis, toxic and drug-induced liver diseases. It promotes protein synthesis, helps in regenerating liver tissue, controls inflammation, enhances glucuronidation, and protects against glutathione depletion. Vitamin E is a well-known antioxidant and has hepatoprotective effect in liver diseases. In this study, we investigated the cytotoxic effects of Ag NPs on primary liver cells of mice. Cell viability (cytotoxicity) was examined with MTT assay after primary liver cells of mice exposure to AgNPs at 1, 10, 50, 100, 150, 200, 400 ppm for 24h. AgNPs caused a concentration- dependent decrease of cell viability (IC50 value = 121.7 ppm or µg/ml). Then the hepatoprotective effect of silymarin and vitamin E were experimented on silver nanoparticle toxicity on mice liver primary cell culture. The results showed that silymarin at 600µg/ml and vitamin E at 2500µmol/l have protective effects on silver nanoparticle toxicity on mice liver primary cell culture. Viability percentage of the primary liver cell of the mouse were exposed to silver nanoparticles at 121.7ppm and co-treatment of silymarin, and vitamin E is more than viability percentage of the primary liver cell of the mouse were exposed to silver nanoparticles and silymarin or silver nanoparticles and vitamin E. |
| first_indexed | 2024-12-10T17:39:22Z |
| format | Article |
| id | doaj.art-5a3a6fc68add4abbb44b2b5469192147 |
| institution | Directory Open Access Journal |
| issn | 0044-6025 1735-9694 |
| language | English |
| last_indexed | 2024-12-10T17:39:22Z |
| publishDate | 2016-03-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Acta Medica Iranica |
| spelling | doaj.art-5a3a6fc68add4abbb44b2b54691921472022-12-22T01:39:26ZengTehran University of Medical SciencesActa Medica Iranica0044-60251735-96942016-03-015424957Study of Silymarin and Vitamin E Protective Effects on Silver Nanoparticle Toxicity on Mice Liver Primary Cell CultureFirouz Faedmaleki0Farshad H Shirazi1Shahram Ejtemaeimehr2Soghra Anjarani3Amir-Ahmad Salarian4Hamidreza Ahmadi Ashtiani5Hossein Rastegar6Department of Basic Science, Science and Research Branch, Islamic Azad University, Tehran, Iran.Pharmaceutical Sciences Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.Department of Pharmacology, Tehran University of Medical Sciences, Tehran, Iran.Health Reference laboratories, Ministry of Health and Medical Education, Tehran, Iran.5Deptartment of Toxicology, Aja University of Medical Science, Tehran, Iran.Pharmaceutical Sciences Branch, Islamic Azad University, Tehran, Iran. AND Cosmetic Products Research Center, Iran Food and Drug Adminstration, Ministry of Health and Medical Education, Tehran, Iran.Department of Basic Science, Science and Research Branch, Islamic Azad University, Tehran, Iran. AND Pharmaceutical Sciences Branch, Islamic Azad University, Tehran, Iran. AND Cosmetic Products Research Center, Iran Food and Drug Adminstration, Ministry of Health and Medical Education, Tehran, Iran.Nanotechnology is a most promising field for generating new applications in medicine, although, only few nano products are currently in use for medical purposes. A most prominent nanoproduct is nanosilver. Nano-silver has biological properties which are significant for consumer products, food technology, textiles, and medical applications (e.g. wound care products, implantable medical devices, in diagnosis, drug delivery, and imaging). For their antibacterial activity, silver nanoparticles (Ag NPs) are largely used in various commercially available products. The use of nano-silver is becoming more and more widespread in medicine and related applications, and due to its increasing exposure, toxicological and environmental issues need to be raised. Cytotoxicity induced by silver nanoparticles (AgNPs) and the role that oxidative stress plays in this process were demonstrated in human hepatoma cells AgNPs agglomerated in the cytoplasm and nuclei of treated cells, and they induced intracellular oxidative stress. AgNP reduced ATP content of the cell and caused damage to mitochondria and increased production of reactive oxygen species (ROS) in a dose-dependent manner. Silymarin was known as a hepatoprotective agent that is used in the treatment of hepatic diseases including viral hepatitis, alcoholic liver diseases, Amanita mushroom poisoning, liver cirrhosis, toxic and drug-induced liver diseases. It promotes protein synthesis, helps in regenerating liver tissue, controls inflammation, enhances glucuronidation, and protects against glutathione depletion. Vitamin E is a well-known antioxidant and has hepatoprotective effect in liver diseases. In this study, we investigated the cytotoxic effects of Ag NPs on primary liver cells of mice. Cell viability (cytotoxicity) was examined with MTT assay after primary liver cells of mice exposure to AgNPs at 1, 10, 50, 100, 150, 200, 400 ppm for 24h. AgNPs caused a concentration- dependent decrease of cell viability (IC50 value = 121.7 ppm or µg/ml). Then the hepatoprotective effect of silymarin and vitamin E were experimented on silver nanoparticle toxicity on mice liver primary cell culture. The results showed that silymarin at 600µg/ml and vitamin E at 2500µmol/l have protective effects on silver nanoparticle toxicity on mice liver primary cell culture. Viability percentage of the primary liver cell of the mouse were exposed to silver nanoparticles at 121.7ppm and co-treatment of silymarin, and vitamin E is more than viability percentage of the primary liver cell of the mouse were exposed to silver nanoparticles and silymarin or silver nanoparticles and vitamin E.https://acta.tums.ac.ir/index.php/acta/article/view/5493Silver nanoparticleSilymarinVitamin EMTT assay |
| spellingShingle | Firouz Faedmaleki Farshad H Shirazi Shahram Ejtemaeimehr Soghra Anjarani Amir-Ahmad Salarian Hamidreza Ahmadi Ashtiani Hossein Rastegar Study of Silymarin and Vitamin E Protective Effects on Silver Nanoparticle Toxicity on Mice Liver Primary Cell Culture Acta Medica Iranica Silver nanoparticle Silymarin Vitamin E MTT assay |
| title | Study of Silymarin and Vitamin E Protective Effects on Silver Nanoparticle Toxicity on Mice Liver Primary Cell Culture |
| title_full | Study of Silymarin and Vitamin E Protective Effects on Silver Nanoparticle Toxicity on Mice Liver Primary Cell Culture |
| title_fullStr | Study of Silymarin and Vitamin E Protective Effects on Silver Nanoparticle Toxicity on Mice Liver Primary Cell Culture |
| title_full_unstemmed | Study of Silymarin and Vitamin E Protective Effects on Silver Nanoparticle Toxicity on Mice Liver Primary Cell Culture |
| title_short | Study of Silymarin and Vitamin E Protective Effects on Silver Nanoparticle Toxicity on Mice Liver Primary Cell Culture |
| title_sort | study of silymarin and vitamin e protective effects on silver nanoparticle toxicity on mice liver primary cell culture |
| topic | Silver nanoparticle Silymarin Vitamin E MTT assay |
| url | https://acta.tums.ac.ir/index.php/acta/article/view/5493 |
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