Pre-breeding of lentil (Lens culinaris Medik.) for herbicide resistance through seed mutagenesis.
Lentil is a poor competitor of weeds and its sensitivity to herbicides is a major hurdle for large scale production. The present study was conducted to select herbicide resistant lentil genotypes through seed mutagenesis. Seeds of three advanced lentil genotypes (LPP 11001, LPP 11100 and LPP 11116)...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2017-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC5308809?pdf=render |
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| author | Muhammad Rizwan Muhammad Aslam Muhammad Jawad Asghar Ghulam Abbas Tariq Mahmud Shah Hussein Shimelis |
| author_facet | Muhammad Rizwan Muhammad Aslam Muhammad Jawad Asghar Ghulam Abbas Tariq Mahmud Shah Hussein Shimelis |
| author_sort | Muhammad Rizwan |
| collection | DOAJ |
| description | Lentil is a poor competitor of weeds and its sensitivity to herbicides is a major hurdle for large scale production. The present study was conducted to select herbicide resistant lentil genotypes through seed mutagenesis. Seeds of three advanced lentil genotypes (LPP 11001, LPP 11100 and LPP 11116) were treated with two different concentrations of ethyl methanesulfonate (EMS; 0.1 and 0.2%), hydrazine hydrate (HH; 0.02 and 0.03%) and sodium azide (SA; 0.01 and 0.02%) to develop M1 seed. The M2 was screened against two herbicides including Ally Max 28.6% SG (X = 34.58 g/ha and 1.5X = 51.87 g/ha) and Atlantis 3.6% WG (X = 395.2 g/ha and 1.5X = 592.8 g/ha) using the following three screening methods: post plant emergence (PPE), pre-plant incorporation (PPI) and seed priming (SP). Data were recorded on survival index and survival percentage from each experimental unit of every population. Plants in all populations were categorized following their reaction to herbicides. The newly developed populations showed greater variation for herbicide resistance when compared to their progenitors. Phenotypic traits were significantly reduced in all the screening environments. Overall, 671 herbicide resistant mutants were selected from all testing environments. The seeds from selected plants were re-mutagenized at 150 Gy of gamma radiation and evaluated against higher dose of herbicides. This allowed selection of 134 herbicide resistant mutants. The selected mutants are useful germplasm for herbicide resistance breeding of lentil. |
| first_indexed | 2024-12-10T17:40:25Z |
| format | Article |
| id | doaj.art-5a3ce01c21c54c7fadc2829eb8bd3952 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-10T17:40:25Z |
| publishDate | 2017-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-5a3ce01c21c54c7fadc2829eb8bd39522022-12-22T01:39:23ZengPublic Library of Science (PLoS)PLoS ONE1932-62032017-01-01122e017184610.1371/journal.pone.0171846Pre-breeding of lentil (Lens culinaris Medik.) for herbicide resistance through seed mutagenesis.Muhammad RizwanMuhammad AslamMuhammad Jawad AsgharGhulam AbbasTariq Mahmud ShahHussein ShimelisLentil is a poor competitor of weeds and its sensitivity to herbicides is a major hurdle for large scale production. The present study was conducted to select herbicide resistant lentil genotypes through seed mutagenesis. Seeds of three advanced lentil genotypes (LPP 11001, LPP 11100 and LPP 11116) were treated with two different concentrations of ethyl methanesulfonate (EMS; 0.1 and 0.2%), hydrazine hydrate (HH; 0.02 and 0.03%) and sodium azide (SA; 0.01 and 0.02%) to develop M1 seed. The M2 was screened against two herbicides including Ally Max 28.6% SG (X = 34.58 g/ha and 1.5X = 51.87 g/ha) and Atlantis 3.6% WG (X = 395.2 g/ha and 1.5X = 592.8 g/ha) using the following three screening methods: post plant emergence (PPE), pre-plant incorporation (PPI) and seed priming (SP). Data were recorded on survival index and survival percentage from each experimental unit of every population. Plants in all populations were categorized following their reaction to herbicides. The newly developed populations showed greater variation for herbicide resistance when compared to their progenitors. Phenotypic traits were significantly reduced in all the screening environments. Overall, 671 herbicide resistant mutants were selected from all testing environments. The seeds from selected plants were re-mutagenized at 150 Gy of gamma radiation and evaluated against higher dose of herbicides. This allowed selection of 134 herbicide resistant mutants. The selected mutants are useful germplasm for herbicide resistance breeding of lentil.http://europepmc.org/articles/PMC5308809?pdf=render |
| spellingShingle | Muhammad Rizwan Muhammad Aslam Muhammad Jawad Asghar Ghulam Abbas Tariq Mahmud Shah Hussein Shimelis Pre-breeding of lentil (Lens culinaris Medik.) for herbicide resistance through seed mutagenesis. PLoS ONE |
| title | Pre-breeding of lentil (Lens culinaris Medik.) for herbicide resistance through seed mutagenesis. |
| title_full | Pre-breeding of lentil (Lens culinaris Medik.) for herbicide resistance through seed mutagenesis. |
| title_fullStr | Pre-breeding of lentil (Lens culinaris Medik.) for herbicide resistance through seed mutagenesis. |
| title_full_unstemmed | Pre-breeding of lentil (Lens culinaris Medik.) for herbicide resistance through seed mutagenesis. |
| title_short | Pre-breeding of lentil (Lens culinaris Medik.) for herbicide resistance through seed mutagenesis. |
| title_sort | pre breeding of lentil lens culinaris medik for herbicide resistance through seed mutagenesis |
| url | http://europepmc.org/articles/PMC5308809?pdf=render |
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