Influence of organic solvents on the furin activity
Furin belongs to a family of calcium-dependent serine proprotein convertases, which transform the inactive protein precursors into mature polypeptides. In model experiments, we studied the effect of organic solvents such as acetone, dimethyl sulfoxide (DMSO), dioxane, isopropanol and ethanol on the...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry
2018-10-01
|
| Series: | The Ukrainian Biochemical Journal |
| Subjects: | |
| Online Access: | http://ukrbiochemjournal.org/2018/09/influence-of-organic-solvents-on-the-furin-activity.html |
| _version_ | 1797427420151676928 |
|---|---|
| author | T. V. Osadchuk O. V. Shybyryn A. V. Semyroz V. K. Kibirev |
| author_facet | T. V. Osadchuk O. V. Shybyryn A. V. Semyroz V. K. Kibirev |
| author_sort | T. V. Osadchuk |
| collection | DOAJ |
| description | Furin belongs to a family of calcium-dependent serine proprotein convertases, which transform the inactive protein precursors into mature polypeptides. In model experiments, we studied the effect of organic solvents such as acetone, dimethyl sulfoxide (DMSO), dioxane, isopropanol and ethanol on the furin activity. Furin was found to retain up to 88% of its initial activity in the presence of DMSO, whereas in the presence of acetone only 30%. Organic solvents formed the following decreasing sequence of their effects on furin: acetone> isopropanol> ethanol> dioxane> dimethyl sulfoxide. The relationship between the residual furin activity and solvent parameters such as relative polarity, dipole moment and log P were investigated. The effect of the organic solvent appeared not to correlate with any of the listed characteristics. Laidler-Sсatchard’s graphs, which according to a theory must be linear, demostrated non-linearity. These results indicate that not only electrostatic interactions play an important role in the studied enzymatic reaction but also other factors, e.g. hydrophobic contacts, hydrogen bonds can influence furin catalysis. This seems relevant for further research in this area. |
| first_indexed | 2024-03-09T08:43:51Z |
| format | Article |
| id | doaj.art-5a41a16bb75c484f8b5d1d2195dafd3a |
| institution | Directory Open Access Journal |
| issn | 2409-4943 2413-5003 |
| language | English |
| last_indexed | 2024-03-09T08:43:51Z |
| publishDate | 2018-10-01 |
| publisher | National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry |
| record_format | Article |
| series | The Ukrainian Biochemical Journal |
| spelling | doaj.art-5a41a16bb75c484f8b5d1d2195dafd3a2023-12-02T15:44:21ZengNational Academy of Sciences of Ukraine, Palladin Institute of BiochemistryThe Ukrainian Biochemical Journal2409-49432413-50032018-10-01905283310.15407/ubj90.05.028Influence of organic solvents on the furin activityT. V. Osadchuk0O. V. Shybyryn1A. V. Semyroz2V. K. Kibirev3Institute of Bioorganic Chemistry and Petrochemistry, National Academy of Sciences of Ukraine, KyivInstitute of Bioorganic Chemistry and Petrochemistry, National Academy of Sciences of Ukraine, KyivInstitute of Bioorganic Chemistry and Petrochemistry, National Academy of Sciences of Ukraine, KyivInstitute of Bioorganic Chemistry and Petrochemistry, National Academy of Sciences of Ukraine, Kyiv;;Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, KyivFurin belongs to a family of calcium-dependent serine proprotein convertases, which transform the inactive protein precursors into mature polypeptides. In model experiments, we studied the effect of organic solvents such as acetone, dimethyl sulfoxide (DMSO), dioxane, isopropanol and ethanol on the furin activity. Furin was found to retain up to 88% of its initial activity in the presence of DMSO, whereas in the presence of acetone only 30%. Organic solvents formed the following decreasing sequence of their effects on furin: acetone> isopropanol> ethanol> dioxane> dimethyl sulfoxide. The relationship between the residual furin activity and solvent parameters such as relative polarity, dipole moment and log P were investigated. The effect of the organic solvent appeared not to correlate with any of the listed characteristics. Laidler-Sсatchard’s graphs, which according to a theory must be linear, demostrated non-linearity. These results indicate that not only electrostatic interactions play an important role in the studied enzymatic reaction but also other factors, e.g. hydrophobic contacts, hydrogen bonds can influence furin catalysis. This seems relevant for further research in this area.http://ukrbiochemjournal.org/2018/09/influence-of-organic-solvents-on-the-furin-activity.htmlactivationfurinfurin activityinhibitionorganic solvents |
| spellingShingle | T. V. Osadchuk O. V. Shybyryn A. V. Semyroz V. K. Kibirev Influence of organic solvents on the furin activity The Ukrainian Biochemical Journal activation furin furin activity inhibition organic solvents |
| title | Influence of organic solvents on the furin activity |
| title_full | Influence of organic solvents on the furin activity |
| title_fullStr | Influence of organic solvents on the furin activity |
| title_full_unstemmed | Influence of organic solvents on the furin activity |
| title_short | Influence of organic solvents on the furin activity |
| title_sort | influence of organic solvents on the furin activity |
| topic | activation furin furin activity inhibition organic solvents |
| url | http://ukrbiochemjournal.org/2018/09/influence-of-organic-solvents-on-the-furin-activity.html |
| work_keys_str_mv | AT tvosadchuk influenceoforganicsolventsonthefurinactivity AT ovshybyryn influenceoforganicsolventsonthefurinactivity AT avsemyroz influenceoforganicsolventsonthefurinactivity AT vkkibirev influenceoforganicsolventsonthefurinactivity |