Mechanisms of Small Intestine Involvement in Obesity-Induced Atherosclerosis
Xiaoyu Pan,1,2 Zhuoya Jia,1,2 Ruoxi Zhen,1,2 Lin Yue,3 Shu Niu,1,4 Jiangli Ban,2 Shuchun Chen1,2 1Department of Internal Medicine, Hebei Medical University, Shijiazhuang, Hebei, People’s Republic of China; 2Department of Endocrinology, Hebei General Hospital, Shijiazhuang, Hebei, People’s Republic o...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Dove Medical Press
2023-06-01
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| Series: | Diabetes, Metabolic Syndrome and Obesity |
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| Online Access: | https://www.dovepress.com/mechanisms-of-small-intestine-involvement-in-obesity-induced-atheroscl-peer-reviewed-fulltext-article-DMSO |
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| author | Pan X Jia Z Zhen R Yue L Niu S Ban J Chen S |
| author_facet | Pan X Jia Z Zhen R Yue L Niu S Ban J Chen S |
| author_sort | Pan X |
| collection | DOAJ |
| description | Xiaoyu Pan,1,2 Zhuoya Jia,1,2 Ruoxi Zhen,1,2 Lin Yue,3 Shu Niu,1,4 Jiangli Ban,2 Shuchun Chen1,2 1Department of Internal Medicine, Hebei Medical University, Shijiazhuang, Hebei, People’s Republic of China; 2Department of Endocrinology, Hebei General Hospital, Shijiazhuang, Hebei, People’s Republic of China; 3Department of Endocrinology, The Third Hospital of Shijiazhuang, Shijiazhuang, Hebei, People’s Republic of China; 4Department of Endocrinology, Shijiazhuang People’s Hospital, Shijiazhuang, Hebei, People’s Republic of ChinaCorrespondence: Shuchun Chen, Department of Endocrinology, Hebei General Hospital, Shijiazhuang, People’s Republic of China, Tel/Fax +86 31185988406, Email chenshuc2014@163.comPurpose: Studies have shown that atherosclerotic plaques are associated with changes in the microbial composition of the intestinal flora and obesity, and that the small intestine plays an irreplaceable role in regulating intestinal flora homeostasis, but the role of the small intestine in the development of obesity-related atherosclerosis remains understudied. Therefore, this study explores the role of the small intestine in obesity-induced atherosclerosis and its molecular mechanisms.Methods: In the GSE59054 data, small intestine tissue samples from 3 normal and 3 obese mice were analyzed using bioinformatics methods. Screening for differentially expressed genes (DEGs) using the GEO2R tool. The DEGs were next processed for bioinformatics analysis. We constructed an obese mouse model and measured aortic arch pulse wave velocity (PWV). Aortic and small intestine tissues were stained with hematoxylin-eosin (HE) to observe pathological changes. Finally, immunohistochemistry was performed to verify the expression of small intestinal proteins.Results: We identified a total of 122 DEGs. Pathway analysis revealed that BMP4, CDH5, IL1A, NQO1, GSTM1, GSTA3, CAV1 and MGST2 were mainly enriched in the Fluid shear stress and atherosclerosis pathway. In addition, BMP4, NQO1 and GSTM1 are closely related to atherosclerosis. Ultrasound and pathological findings suggest the presence of obesity atherosclerosis. Immunohistochemistry verified high expression of BMP4 and low expression of NQO1 and GSTM1 in obese small intestine tissues.Conclusion: The altered expression of BMP4, NQO1 and GSTM1 in small intestine tissues during obesity may be related to atherosclerosis, and Fluid shear stress and atherosclerosis pathway may be the molecular mechanism of their role.Keywords: obesity, differentially expressed genes, atherosclerosis, small intestine, bioinformatics analysis |
| first_indexed | 2024-03-13T01:49:31Z |
| format | Article |
| id | doaj.art-5a4496f159a346c19417b2ef5ee5c4ec |
| institution | Directory Open Access Journal |
| issn | 1178-7007 |
| language | English |
| last_indexed | 2024-03-13T01:49:31Z |
| publishDate | 2023-06-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | Diabetes, Metabolic Syndrome and Obesity |
| spelling | doaj.art-5a4496f159a346c19417b2ef5ee5c4ec2023-07-02T19:49:17ZengDove Medical PressDiabetes, Metabolic Syndrome and Obesity1178-70072023-06-01Volume 161941195284755Mechanisms of Small Intestine Involvement in Obesity-Induced AtherosclerosisPan XJia ZZhen RYue LNiu SBan JChen SXiaoyu Pan,1,2 Zhuoya Jia,1,2 Ruoxi Zhen,1,2 Lin Yue,3 Shu Niu,1,4 Jiangli Ban,2 Shuchun Chen1,2 1Department of Internal Medicine, Hebei Medical University, Shijiazhuang, Hebei, People’s Republic of China; 2Department of Endocrinology, Hebei General Hospital, Shijiazhuang, Hebei, People’s Republic of China; 3Department of Endocrinology, The Third Hospital of Shijiazhuang, Shijiazhuang, Hebei, People’s Republic of China; 4Department of Endocrinology, Shijiazhuang People’s Hospital, Shijiazhuang, Hebei, People’s Republic of ChinaCorrespondence: Shuchun Chen, Department of Endocrinology, Hebei General Hospital, Shijiazhuang, People’s Republic of China, Tel/Fax +86 31185988406, Email chenshuc2014@163.comPurpose: Studies have shown that atherosclerotic plaques are associated with changes in the microbial composition of the intestinal flora and obesity, and that the small intestine plays an irreplaceable role in regulating intestinal flora homeostasis, but the role of the small intestine in the development of obesity-related atherosclerosis remains understudied. Therefore, this study explores the role of the small intestine in obesity-induced atherosclerosis and its molecular mechanisms.Methods: In the GSE59054 data, small intestine tissue samples from 3 normal and 3 obese mice were analyzed using bioinformatics methods. Screening for differentially expressed genes (DEGs) using the GEO2R tool. The DEGs were next processed for bioinformatics analysis. We constructed an obese mouse model and measured aortic arch pulse wave velocity (PWV). Aortic and small intestine tissues were stained with hematoxylin-eosin (HE) to observe pathological changes. Finally, immunohistochemistry was performed to verify the expression of small intestinal proteins.Results: We identified a total of 122 DEGs. Pathway analysis revealed that BMP4, CDH5, IL1A, NQO1, GSTM1, GSTA3, CAV1 and MGST2 were mainly enriched in the Fluid shear stress and atherosclerosis pathway. In addition, BMP4, NQO1 and GSTM1 are closely related to atherosclerosis. Ultrasound and pathological findings suggest the presence of obesity atherosclerosis. Immunohistochemistry verified high expression of BMP4 and low expression of NQO1 and GSTM1 in obese small intestine tissues.Conclusion: The altered expression of BMP4, NQO1 and GSTM1 in small intestine tissues during obesity may be related to atherosclerosis, and Fluid shear stress and atherosclerosis pathway may be the molecular mechanism of their role.Keywords: obesity, differentially expressed genes, atherosclerosis, small intestine, bioinformatics analysishttps://www.dovepress.com/mechanisms-of-small-intestine-involvement-in-obesity-induced-atheroscl-peer-reviewed-fulltext-article-DMSOobesitydifferentially expressed genesatherosclerosissmall intestinebioinformatics analysis |
| spellingShingle | Pan X Jia Z Zhen R Yue L Niu S Ban J Chen S Mechanisms of Small Intestine Involvement in Obesity-Induced Atherosclerosis Diabetes, Metabolic Syndrome and Obesity obesity differentially expressed genes atherosclerosis small intestine bioinformatics analysis |
| title | Mechanisms of Small Intestine Involvement in Obesity-Induced Atherosclerosis |
| title_full | Mechanisms of Small Intestine Involvement in Obesity-Induced Atherosclerosis |
| title_fullStr | Mechanisms of Small Intestine Involvement in Obesity-Induced Atherosclerosis |
| title_full_unstemmed | Mechanisms of Small Intestine Involvement in Obesity-Induced Atherosclerosis |
| title_short | Mechanisms of Small Intestine Involvement in Obesity-Induced Atherosclerosis |
| title_sort | mechanisms of small intestine involvement in obesity induced atherosclerosis |
| topic | obesity differentially expressed genes atherosclerosis small intestine bioinformatics analysis |
| url | https://www.dovepress.com/mechanisms-of-small-intestine-involvement-in-obesity-induced-atheroscl-peer-reviewed-fulltext-article-DMSO |
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