Evaluation of P5CS and ProDH activity in Paulownia tomentosa (Steud.) as an indicator of oxidative changes induced by drought stress
The aim of the study was to investigate changes in proline metabolism in seedlings of tree species during drought stress. One month old Paulownia tomentosa seedlings were exposed to moisture conditions at various levels (irrigation at 100, 75, 50 and 25% of field capacity), and then the material (le...
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PeerJ Inc.
2024-01-01
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| author | Joanna Kijowska-Oberc Mikołaj K. Wawrzyniak Liliana Ciszewska Ewelina Ratajczak |
| author_facet | Joanna Kijowska-Oberc Mikołaj K. Wawrzyniak Liliana Ciszewska Ewelina Ratajczak |
| author_sort | Joanna Kijowska-Oberc |
| collection | DOAJ |
| description | The aim of the study was to investigate changes in proline metabolism in seedlings of tree species during drought stress. One month old Paulownia tomentosa seedlings were exposed to moisture conditions at various levels (irrigation at 100, 75, 50 and 25% of field capacity), and then the material (leaves and roots) was collected three times at 10-day intervals. The activity of enzymes involved in proline metabolism was closely related to drought severity; however, proline content was not directly impacted. The activity of pyrroline-5-carboxylate synthetase (P5CS), which catalyzes proline biosynthesis, increased in response to hydrogen peroxide accumulation, which was correlated with soil moisture. In contrast, the activity of proline dehydrogenase (ProDH), which catalyzes proline catabolism, decreased. Compared to proline, the activity of these enzymes may be a more reliable biochemical marker of stress-induced oxidative changes. The content of proline is dependent on numerous additional factors, i.e., its degradation is an important alternative energy source. Moreover, we noted tissue-specific differences in this species, in which roots appeared to be proline biosynthesis sites and leaves appeared to be proline catabolism sites. Further research is needed to examine a broader view of proline metabolism as a cycle regulated by multiple mechanisms and differences between species. |
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| format | Article |
| id | doaj.art-5a4ec770b37943f8bffa2bf077422269 |
| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-08T10:22:38Z |
| publishDate | 2024-01-01 |
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| spelling | doaj.art-5a4ec770b37943f8bffa2bf0774222692024-01-27T15:05:17ZengPeerJ Inc.PeerJ2167-83592024-01-0112e1669710.7717/peerj.16697Evaluation of P5CS and ProDH activity in Paulownia tomentosa (Steud.) as an indicator of oxidative changes induced by drought stressJoanna Kijowska-Oberc0Mikołaj K. Wawrzyniak1Liliana Ciszewska2Ewelina Ratajczak3Institute of Dendrology, Polish Academy of Sciences, Kórnik, Wielkopolskie, PolskaInstitute of Dendrology, Polish Academy of Sciences, Kórnik, Wielkopolskie, PolskaLaboratory of RNA Biochemistry, Institute of Molecular Biology and Biotechnology, Faculty of Biology, Adam Mickiewicz University in Poznan, Poznan, Wielkopolskie, PolskaInstitute of Dendrology, Polish Academy of Sciences, Kórnik, Wielkopolskie, PolskaThe aim of the study was to investigate changes in proline metabolism in seedlings of tree species during drought stress. One month old Paulownia tomentosa seedlings were exposed to moisture conditions at various levels (irrigation at 100, 75, 50 and 25% of field capacity), and then the material (leaves and roots) was collected three times at 10-day intervals. The activity of enzymes involved in proline metabolism was closely related to drought severity; however, proline content was not directly impacted. The activity of pyrroline-5-carboxylate synthetase (P5CS), which catalyzes proline biosynthesis, increased in response to hydrogen peroxide accumulation, which was correlated with soil moisture. In contrast, the activity of proline dehydrogenase (ProDH), which catalyzes proline catabolism, decreased. Compared to proline, the activity of these enzymes may be a more reliable biochemical marker of stress-induced oxidative changes. The content of proline is dependent on numerous additional factors, i.e., its degradation is an important alternative energy source. Moreover, we noted tissue-specific differences in this species, in which roots appeared to be proline biosynthesis sites and leaves appeared to be proline catabolism sites. Further research is needed to examine a broader view of proline metabolism as a cycle regulated by multiple mechanisms and differences between species.https://peerj.com/articles/16697.pdfProline metabolismDrought stressOxidative changesP5CSProDHTree seedlings |
| spellingShingle | Joanna Kijowska-Oberc Mikołaj K. Wawrzyniak Liliana Ciszewska Ewelina Ratajczak Evaluation of P5CS and ProDH activity in Paulownia tomentosa (Steud.) as an indicator of oxidative changes induced by drought stress PeerJ Proline metabolism Drought stress Oxidative changes P5CS ProDH Tree seedlings |
| title | Evaluation of P5CS and ProDH activity in Paulownia tomentosa (Steud.) as an indicator of oxidative changes induced by drought stress |
| title_full | Evaluation of P5CS and ProDH activity in Paulownia tomentosa (Steud.) as an indicator of oxidative changes induced by drought stress |
| title_fullStr | Evaluation of P5CS and ProDH activity in Paulownia tomentosa (Steud.) as an indicator of oxidative changes induced by drought stress |
| title_full_unstemmed | Evaluation of P5CS and ProDH activity in Paulownia tomentosa (Steud.) as an indicator of oxidative changes induced by drought stress |
| title_short | Evaluation of P5CS and ProDH activity in Paulownia tomentosa (Steud.) as an indicator of oxidative changes induced by drought stress |
| title_sort | evaluation of p5cs and prodh activity in paulownia tomentosa steud as an indicator of oxidative changes induced by drought stress |
| topic | Proline metabolism Drought stress Oxidative changes P5CS ProDH Tree seedlings |
| url | https://peerj.com/articles/16697.pdf |
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