Effect of drought stress on male fertility restoration in A3 CMS-inducing cytoplasm of sorghum
Use of cytoplasmic male sterility (CMS) in hybrid breeding requires effective male fertility-restoring lines. In sorghum, very few restoring lines that can restore fertility in A3 CMS have been reported. To identify the reasons for this deficiency, F1 and F2 hybrids of an A3 CMS line crossed with th...
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| Format: | Article |
| Language: | English |
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KeAi Communications Co., Ltd.
2017-08-01
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| Series: | Crop Journal |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2214514117300168 |
| _version_ | 1819117129190866944 |
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| author | Valentin V. Kozhemyakin Lev A. Elkonin Jeffery A. Dahlberg |
| author_facet | Valentin V. Kozhemyakin Lev A. Elkonin Jeffery A. Dahlberg |
| author_sort | Valentin V. Kozhemyakin |
| collection | DOAJ |
| description | Use of cytoplasmic male sterility (CMS) in hybrid breeding requires effective male fertility-restoring lines. In sorghum, very few restoring lines that can restore fertility in A3 CMS have been reported. To identify the reasons for this deficiency, F1 and F2 hybrids of an A3 CMS line crossed with the line IS1112C, a donor of fertility-restoring (Rf) genes for A3 cytoplasm, and testcrosses of fertile plants to A3 CMS lines were grown under contrasting water availability regimes in dryland and irrigated field plots. In the irrigated plots the frequency of fertile plants in testcrosses was twice that in dryland plots (P < 0.05). Fertile plants from the F2 family grown in the irrigated plots showed significantly higher restoration ability than fertile plants from the same family grown in dryland plots. F3 plants from the F2 family grown in irrigated plots yielded on average a sixfold higher frequency of fertile plants in testcrosses than F3 plants derived from dryland plots (P < 0.01). Fertility of testcross hybrids correlated negatively with air vapor pressure deficit (VPD) at flowering (r = −0.96; P < 0.01) suggesting that VPD is a trigger for downregulation of Rf genes for A3 cytoplasm. |
| first_indexed | 2024-12-22T05:28:04Z |
| format | Article |
| id | doaj.art-5a4f0c441b6d4041aa927c8585955b42 |
| institution | Directory Open Access Journal |
| issn | 2214-5141 |
| language | English |
| last_indexed | 2024-12-22T05:28:04Z |
| publishDate | 2017-08-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Crop Journal |
| spelling | doaj.art-5a4f0c441b6d4041aa927c8585955b422022-12-21T18:37:31ZengKeAi Communications Co., Ltd.Crop Journal2214-51412017-08-015428228910.1016/j.cj.2017.02.003Effect of drought stress on male fertility restoration in A3 CMS-inducing cytoplasm of sorghumValentin V. Kozhemyakin0Lev A. Elkonin1Jeffery A. Dahlberg2Department of Biotechnology, Agricultural Research Institute of South-East Region, Tulaikov Street, 7, Saratov 410010, RussiaDepartment of Biotechnology, Agricultural Research Institute of South-East Region, Tulaikov Street, 7, Saratov 410010, RussiaKearney Agricultural Research & Extension (KARE), 9240 South Riverbend Avenue, Parlier, CA 93648, USAUse of cytoplasmic male sterility (CMS) in hybrid breeding requires effective male fertility-restoring lines. In sorghum, very few restoring lines that can restore fertility in A3 CMS have been reported. To identify the reasons for this deficiency, F1 and F2 hybrids of an A3 CMS line crossed with the line IS1112C, a donor of fertility-restoring (Rf) genes for A3 cytoplasm, and testcrosses of fertile plants to A3 CMS lines were grown under contrasting water availability regimes in dryland and irrigated field plots. In the irrigated plots the frequency of fertile plants in testcrosses was twice that in dryland plots (P < 0.05). Fertile plants from the F2 family grown in the irrigated plots showed significantly higher restoration ability than fertile plants from the same family grown in dryland plots. F3 plants from the F2 family grown in irrigated plots yielded on average a sixfold higher frequency of fertile plants in testcrosses than F3 plants derived from dryland plots (P < 0.01). Fertility of testcross hybrids correlated negatively with air vapor pressure deficit (VPD) at flowering (r = −0.96; P < 0.01) suggesting that VPD is a trigger for downregulation of Rf genes for A3 cytoplasm.http://www.sciencedirect.com/science/article/pii/S2214514117300168Sorghum bicolor (L.) MoenchCytoplasmic male sterilityA3 cytoplasmFertility-restoring genesEpigeneticsVapor pressure deficit |
| spellingShingle | Valentin V. Kozhemyakin Lev A. Elkonin Jeffery A. Dahlberg Effect of drought stress on male fertility restoration in A3 CMS-inducing cytoplasm of sorghum Crop Journal Sorghum bicolor (L.) Moench Cytoplasmic male sterility A3 cytoplasm Fertility-restoring genes Epigenetics Vapor pressure deficit |
| title | Effect of drought stress on male fertility restoration in A3 CMS-inducing cytoplasm of sorghum |
| title_full | Effect of drought stress on male fertility restoration in A3 CMS-inducing cytoplasm of sorghum |
| title_fullStr | Effect of drought stress on male fertility restoration in A3 CMS-inducing cytoplasm of sorghum |
| title_full_unstemmed | Effect of drought stress on male fertility restoration in A3 CMS-inducing cytoplasm of sorghum |
| title_short | Effect of drought stress on male fertility restoration in A3 CMS-inducing cytoplasm of sorghum |
| title_sort | effect of drought stress on male fertility restoration in a3 cms inducing cytoplasm of sorghum |
| topic | Sorghum bicolor (L.) Moench Cytoplasmic male sterility A3 cytoplasm Fertility-restoring genes Epigenetics Vapor pressure deficit |
| url | http://www.sciencedirect.com/science/article/pii/S2214514117300168 |
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