Widespread Changes in the Posttranscriptional Landscape at the Drosophila Oocyte-to-Embryo Transition
The oocyte-to-embryo transition marks the onset of development. The initial phase of this profound change from the differentiated oocyte to the totipotent embryo occurs in the absence of both transcription and mRNA degradation. Here we combine global polysome profiling, ribosome-footprint profiling,...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2014-06-01
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| Series: | Cell Reports |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211124714003775 |
| _version_ | 1818215615760957440 |
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| author | Iva Kronja Bingbing Yuan Stephen W. Eichhorn Kristina Dzeyk Jeroen Krijgsveld David P. Bartel Terry L. Orr-Weaver |
| author_facet | Iva Kronja Bingbing Yuan Stephen W. Eichhorn Kristina Dzeyk Jeroen Krijgsveld David P. Bartel Terry L. Orr-Weaver |
| author_sort | Iva Kronja |
| collection | DOAJ |
| description | The oocyte-to-embryo transition marks the onset of development. The initial phase of this profound change from the differentiated oocyte to the totipotent embryo occurs in the absence of both transcription and mRNA degradation. Here we combine global polysome profiling, ribosome-footprint profiling, and quantitative mass spectrometry in a comprehensive approach to delineate the translational and proteomic changes that occur during this important transition in Drosophila. Our results show that PNG kinase is a critical regulator of the extensive changes in the translatome, acting uniquely at this developmental window. Analysis of the proteome in png mutants provided insights into the contributions of translation to changes in protein levels, revealing a compensatory dynamic between translation and protein turnover during proteome remodeling at the return to totipotency. The proteome changes additionally suggested regulators of meiosis and early embryogenesis, including the conserved H3K4 demethylase LID, which we demonstrated is required during this period despite transcriptional inactivity. |
| first_indexed | 2024-12-12T06:38:54Z |
| format | Article |
| id | doaj.art-5a500ff43cba459da50ecf4d9b280b89 |
| institution | Directory Open Access Journal |
| issn | 2211-1247 |
| language | English |
| last_indexed | 2024-12-12T06:38:54Z |
| publishDate | 2014-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Cell Reports |
| spelling | doaj.art-5a500ff43cba459da50ecf4d9b280b892022-12-22T00:34:24ZengElsevierCell Reports2211-12472014-06-01751495150810.1016/j.celrep.2014.05.002Widespread Changes in the Posttranscriptional Landscape at the Drosophila Oocyte-to-Embryo TransitionIva Kronja0Bingbing Yuan1Stephen W. Eichhorn2Kristina Dzeyk3Jeroen Krijgsveld4David P. Bartel5Terry L. Orr-Weaver6Whitehead Institute, Cambridge, MA 02142, USAWhitehead Institute, Cambridge, MA 02142, USAWhitehead Institute, Cambridge, MA 02142, USAEuropean Molecular Biology Laboratory, 69117 Heidelberg, GermanyEuropean Molecular Biology Laboratory, 69117 Heidelberg, GermanyWhitehead Institute, Cambridge, MA 02142, USAWhitehead Institute, Cambridge, MA 02142, USAThe oocyte-to-embryo transition marks the onset of development. The initial phase of this profound change from the differentiated oocyte to the totipotent embryo occurs in the absence of both transcription and mRNA degradation. Here we combine global polysome profiling, ribosome-footprint profiling, and quantitative mass spectrometry in a comprehensive approach to delineate the translational and proteomic changes that occur during this important transition in Drosophila. Our results show that PNG kinase is a critical regulator of the extensive changes in the translatome, acting uniquely at this developmental window. Analysis of the proteome in png mutants provided insights into the contributions of translation to changes in protein levels, revealing a compensatory dynamic between translation and protein turnover during proteome remodeling at the return to totipotency. The proteome changes additionally suggested regulators of meiosis and early embryogenesis, including the conserved H3K4 demethylase LID, which we demonstrated is required during this period despite transcriptional inactivity.http://www.sciencedirect.com/science/article/pii/S2211124714003775 |
| spellingShingle | Iva Kronja Bingbing Yuan Stephen W. Eichhorn Kristina Dzeyk Jeroen Krijgsveld David P. Bartel Terry L. Orr-Weaver Widespread Changes in the Posttranscriptional Landscape at the Drosophila Oocyte-to-Embryo Transition Cell Reports |
| title | Widespread Changes in the Posttranscriptional Landscape at the Drosophila Oocyte-to-Embryo Transition |
| title_full | Widespread Changes in the Posttranscriptional Landscape at the Drosophila Oocyte-to-Embryo Transition |
| title_fullStr | Widespread Changes in the Posttranscriptional Landscape at the Drosophila Oocyte-to-Embryo Transition |
| title_full_unstemmed | Widespread Changes in the Posttranscriptional Landscape at the Drosophila Oocyte-to-Embryo Transition |
| title_short | Widespread Changes in the Posttranscriptional Landscape at the Drosophila Oocyte-to-Embryo Transition |
| title_sort | widespread changes in the posttranscriptional landscape at the drosophila oocyte to embryo transition |
| url | http://www.sciencedirect.com/science/article/pii/S2211124714003775 |
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