Functional Polymorphism in the <i>ADRB3</i> Gene, Encoding the Beta-3 Adrenergic Receptor, and Response to Intra-Detrusor Injection of Botulinum Toxin-A in Women with Overactive Bladder

Background: There are reports suggesting an association between the rs4994 polymorphism in the <i>ADRB3</i> gene encoding the beta-3 adrenergic receptor and OAB risk in females. The injection of botulinum toxin-A into the bladder wall is recommended as a possible treatment for OAB patien...

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Main Authors: Sylwester Michał Ciećwież, Klaudyna Lewandowska, Agnieszka Boroń, Jacek Brodowski, Jacek Kociszewski, Jeremy Simon Clark, Andrzej Ciechanowicz
Format: Article
Language:English
Published: MDPI AG 2022-12-01
Series:Journal of Clinical Medicine
Subjects:
Online Access:https://www.mdpi.com/2077-0383/11/24/7491
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author Sylwester Michał Ciećwież
Klaudyna Lewandowska
Agnieszka Boroń
Jacek Brodowski
Jacek Kociszewski
Jeremy Simon Clark
Andrzej Ciechanowicz
author_facet Sylwester Michał Ciećwież
Klaudyna Lewandowska
Agnieszka Boroń
Jacek Brodowski
Jacek Kociszewski
Jeremy Simon Clark
Andrzej Ciechanowicz
author_sort Sylwester Michał Ciećwież
collection DOAJ
description Background: There are reports suggesting an association between the rs4994 polymorphism in the <i>ADRB3</i> gene encoding the beta-3 adrenergic receptor and OAB risk in females. The injection of botulinum toxin-A into the bladder wall is recommended as a possible treatment for OAB patients in whom first-line therapies have failed. The aim of our study was to analyze the possible association between the <i>ADRB3</i>:rs4994 polymorphism and the patient-perceived response to a single intra-detrusor injection of botulinum toxin-A in Polish women with overactive bladder. Methods: The study group consisted of 115 consecutive female patients with OAB. The response to botulinum toxin-A was evaluated at three months after injection, as absolute or relative reductions in OAB symptoms or in scores from questionnaires ICIQ-OAB (parts A and B) and ICIQ-LUTS-QoL (parts A and B). <i>ADRB3</i>:rs4994 variants were identified by the sequencing of genomic DNA extracted from buccal swabs. Results: There were no statistically significant differences between <i>ADRB3</i>:rs4994 [T];[T] homozygotes and [T];[C]+[C];[C] subjects for absolute or relative reductions in symptoms or in scores from all four questionnaire parts at three months after the injection of botulinum toxin-A. Conclusions: Our results do not support the hypothesis that <i>ADRB3</i>:rs4994 polymorphism is associated with the response to the intra-detrusor injection of botulinum toxin-A in Polish females with overactive bladder.
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spelling doaj.art-5a52420529e44264988a70685b26e21a2023-11-24T15:46:30ZengMDPI AGJournal of Clinical Medicine2077-03832022-12-011124749110.3390/jcm11247491Functional Polymorphism in the <i>ADRB3</i> Gene, Encoding the Beta-3 Adrenergic Receptor, and Response to Intra-Detrusor Injection of Botulinum Toxin-A in Women with Overactive BladderSylwester Michał Ciećwież0Klaudyna Lewandowska1Agnieszka Boroń2Jacek Brodowski3Jacek Kociszewski4Jeremy Simon Clark5Andrzej Ciechanowicz6Department of Gynecology, Endocrinology and Gynecological Oncology, Pomeranian Medical University, 71-252 Szczecin, PolandDepartment of Clinical and Molecular Biochemistry, Pomeranian Medical University, 70-111 Szczecin, PolandDepartment of Clinical and Molecular Biochemistry, Pomeranian Medical University, 70-111 Szczecin, PolandDepartment of Primary Health Care, Pomeranian Medical University, 70-204 Szczecin, PolandDepartment of Gynecology, Evangelisches Krankenhaus Hagen, 58135 Hagen, GermanyDepartment of Clinical and Molecular Biochemistry, Pomeranian Medical University, 70-111 Szczecin, PolandDepartment of Clinical and Molecular Biochemistry, Pomeranian Medical University, 70-111 Szczecin, PolandBackground: There are reports suggesting an association between the rs4994 polymorphism in the <i>ADRB3</i> gene encoding the beta-3 adrenergic receptor and OAB risk in females. The injection of botulinum toxin-A into the bladder wall is recommended as a possible treatment for OAB patients in whom first-line therapies have failed. The aim of our study was to analyze the possible association between the <i>ADRB3</i>:rs4994 polymorphism and the patient-perceived response to a single intra-detrusor injection of botulinum toxin-A in Polish women with overactive bladder. Methods: The study group consisted of 115 consecutive female patients with OAB. The response to botulinum toxin-A was evaluated at three months after injection, as absolute or relative reductions in OAB symptoms or in scores from questionnaires ICIQ-OAB (parts A and B) and ICIQ-LUTS-QoL (parts A and B). <i>ADRB3</i>:rs4994 variants were identified by the sequencing of genomic DNA extracted from buccal swabs. Results: There were no statistically significant differences between <i>ADRB3</i>:rs4994 [T];[T] homozygotes and [T];[C]+[C];[C] subjects for absolute or relative reductions in symptoms or in scores from all four questionnaire parts at three months after the injection of botulinum toxin-A. Conclusions: Our results do not support the hypothesis that <i>ADRB3</i>:rs4994 polymorphism is associated with the response to the intra-detrusor injection of botulinum toxin-A in Polish females with overactive bladder.https://www.mdpi.com/2077-0383/11/24/7491beta-3 adrenoreceptorbotulinum neurotoxin-Agene polymorphismoveractive bladder
spellingShingle Sylwester Michał Ciećwież
Klaudyna Lewandowska
Agnieszka Boroń
Jacek Brodowski
Jacek Kociszewski
Jeremy Simon Clark
Andrzej Ciechanowicz
Functional Polymorphism in the <i>ADRB3</i> Gene, Encoding the Beta-3 Adrenergic Receptor, and Response to Intra-Detrusor Injection of Botulinum Toxin-A in Women with Overactive Bladder
Journal of Clinical Medicine
beta-3 adrenoreceptor
botulinum neurotoxin-A
gene polymorphism
overactive bladder
title Functional Polymorphism in the <i>ADRB3</i> Gene, Encoding the Beta-3 Adrenergic Receptor, and Response to Intra-Detrusor Injection of Botulinum Toxin-A in Women with Overactive Bladder
title_full Functional Polymorphism in the <i>ADRB3</i> Gene, Encoding the Beta-3 Adrenergic Receptor, and Response to Intra-Detrusor Injection of Botulinum Toxin-A in Women with Overactive Bladder
title_fullStr Functional Polymorphism in the <i>ADRB3</i> Gene, Encoding the Beta-3 Adrenergic Receptor, and Response to Intra-Detrusor Injection of Botulinum Toxin-A in Women with Overactive Bladder
title_full_unstemmed Functional Polymorphism in the <i>ADRB3</i> Gene, Encoding the Beta-3 Adrenergic Receptor, and Response to Intra-Detrusor Injection of Botulinum Toxin-A in Women with Overactive Bladder
title_short Functional Polymorphism in the <i>ADRB3</i> Gene, Encoding the Beta-3 Adrenergic Receptor, and Response to Intra-Detrusor Injection of Botulinum Toxin-A in Women with Overactive Bladder
title_sort functional polymorphism in the i adrb3 i gene encoding the beta 3 adrenergic receptor and response to intra detrusor injection of botulinum toxin a in women with overactive bladder
topic beta-3 adrenoreceptor
botulinum neurotoxin-A
gene polymorphism
overactive bladder
url https://www.mdpi.com/2077-0383/11/24/7491
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