The use of functionalized AFM tips as molecular sensors in the detection of pesticides
Atomic force spectroscopy, a technique derived from Atomic Force Microscopy (AFM), allowed us to distinguish nonspecific and specific interactions between the acetolactate synthase enzyme (ALS) and anti-atrazine antibody biomolecules and the herbicides imazaquin, metsulfuron-methyl and atrazine. The...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Associação Brasileira de Metalurgia e Materiais (ABM); Associação Brasileira de Cerâmica (ABC); Associação Brasileira de Polímeros (ABPol)
2013-06-01
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| Series: | Materials Research |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-14392013000300023 |
| _version_ | 1819209360607281152 |
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| author | Daiana K. Deda Bárbara B. S. Pereira Carolina C. Bueno Aline N. da Silva Gabrielle A. Ribeiro Adriano M. Amarante Eduardo F. Franca Fabio L. Leite |
| author_facet | Daiana K. Deda Bárbara B. S. Pereira Carolina C. Bueno Aline N. da Silva Gabrielle A. Ribeiro Adriano M. Amarante Eduardo F. Franca Fabio L. Leite |
| author_sort | Daiana K. Deda |
| collection | DOAJ |
| description | Atomic force spectroscopy, a technique derived from Atomic Force Microscopy (AFM), allowed us to distinguish nonspecific and specific interactions between the acetolactate synthase enzyme (ALS) and anti-atrazine antibody biomolecules and the herbicides imazaquin, metsulfuron-methyl and atrazine. The presence of specific interactions increased the adhesion force (Fadh) between the AFM tip and the herbicides, which made the modified tip a powerful biosensor. Increases of approximately 132% and 145% in the Fadh values were observed when a tip functionalized with ALS was used to detect imazaquin and metsulfuron-methyl, respectively. The presence of specific interactions between the atrazine and the anti-atrazine antibody also caused an increase in the Fadh values (approximately 175%) compared to those observed when using an unfunctionalized tip. The molecular modeling results obtained with the ALS enzyme suggest that the orientation of the biomolecule on the tip surface could be suitable for allowing interaction with the herbicides imazaquin and metsulfuron-methyl. |
| first_indexed | 2024-12-23T05:54:03Z |
| format | Article |
| id | doaj.art-5a531f3a4fd141589392007980a4ac29 |
| institution | Directory Open Access Journal |
| issn | 1516-1439 |
| language | English |
| last_indexed | 2024-12-23T05:54:03Z |
| publishDate | 2013-06-01 |
| publisher | Associação Brasileira de Metalurgia e Materiais (ABM); Associação Brasileira de Cerâmica (ABC); Associação Brasileira de Polímeros (ABPol) |
| record_format | Article |
| series | Materials Research |
| spelling | doaj.art-5a531f3a4fd141589392007980a4ac292022-12-21T17:57:51ZengAssociação Brasileira de Metalurgia e Materiais (ABM); Associação Brasileira de Cerâmica (ABC); Associação Brasileira de Polímeros (ABPol)Materials Research1516-14392013-06-01163683687The use of functionalized AFM tips as molecular sensors in the detection of pesticidesDaiana K. DedaBárbara B. S. PereiraCarolina C. BuenoAline N. da SilvaGabrielle A. RibeiroAdriano M. AmaranteEduardo F. FrancaFabio L. LeiteAtomic force spectroscopy, a technique derived from Atomic Force Microscopy (AFM), allowed us to distinguish nonspecific and specific interactions between the acetolactate synthase enzyme (ALS) and anti-atrazine antibody biomolecules and the herbicides imazaquin, metsulfuron-methyl and atrazine. The presence of specific interactions increased the adhesion force (Fadh) between the AFM tip and the herbicides, which made the modified tip a powerful biosensor. Increases of approximately 132% and 145% in the Fadh values were observed when a tip functionalized with ALS was used to detect imazaquin and metsulfuron-methyl, respectively. The presence of specific interactions between the atrazine and the anti-atrazine antibody also caused an increase in the Fadh values (approximately 175%) compared to those observed when using an unfunctionalized tip. The molecular modeling results obtained with the ALS enzyme suggest that the orientation of the biomolecule on the tip surface could be suitable for allowing interaction with the herbicides imazaquin and metsulfuron-methyl.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-14392013000300023nanobiosensorschemical force microscopyatomic force spectroscopymolecular modeling |
| spellingShingle | Daiana K. Deda Bárbara B. S. Pereira Carolina C. Bueno Aline N. da Silva Gabrielle A. Ribeiro Adriano M. Amarante Eduardo F. Franca Fabio L. Leite The use of functionalized AFM tips as molecular sensors in the detection of pesticides Materials Research nanobiosensors chemical force microscopy atomic force spectroscopy molecular modeling |
| title | The use of functionalized AFM tips as molecular sensors in the detection of pesticides |
| title_full | The use of functionalized AFM tips as molecular sensors in the detection of pesticides |
| title_fullStr | The use of functionalized AFM tips as molecular sensors in the detection of pesticides |
| title_full_unstemmed | The use of functionalized AFM tips as molecular sensors in the detection of pesticides |
| title_short | The use of functionalized AFM tips as molecular sensors in the detection of pesticides |
| title_sort | use of functionalized afm tips as molecular sensors in the detection of pesticides |
| topic | nanobiosensors chemical force microscopy atomic force spectroscopy molecular modeling |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-14392013000300023 |
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