Development of synbiotic beverage from beetroot juice using beneficial probiotic Lactobacillus Casei 431

A beetroot beverage with Lactobacillus casei 431 as the probiotic microorganism was tested for sensory acceptability at 3 fermentation periods of 2, 4 and 6 hours at 37oC. The beverage fermented for 2 hours yielded the highest overall sensory acceptability. L. casei 431 grew well and reached nearly...

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Main Authors: S.M. Gamage, M.K.S. Mihirani, O.D.A.N. Perera, H.L.D. Weerahewa
Format: Article
Language:English
Published: University of Ruhuna 2016-12-01
Series:Ruhuna Journal of Science
Subjects:
Online Access:https://rjs.sljol.info/articles/10.4038/rjs.v7i2.20/galley/30/download/
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author S.M. Gamage
M.K.S. Mihirani
O.D.A.N. Perera
H.L.D. Weerahewa
author_facet S.M. Gamage
M.K.S. Mihirani
O.D.A.N. Perera
H.L.D. Weerahewa
author_sort S.M. Gamage
collection DOAJ
description A beetroot beverage with Lactobacillus casei 431 as the probiotic microorganism was tested for sensory acceptability at 3 fermentation periods of 2, 4 and 6 hours at 37oC. The beverage fermented for 2 hours yielded the highest overall sensory acceptability. L. casei 431 grew well and reached nearly 108 CFU/ mL after 2 hours of fermentation at 37oC. Although the lactic culture in the fermented beetroot beverage gradually lost its viability during cold storage, viable cell count of lactic acid bacteria remained at 106–108 CFU/mL after 4 weeks of cold storage at 4oC. Titratable acidity increased significantly from 5.5±0.05 to 3.45x108 (P≤ 0.05) during storage. This study concludes that this beetroot-based synbiotic fermented beverage could be developed as a ready-to-drink product and kept for 6 weeks under refrigerated storage, meeting the standard 108–1010 CFU/mL of a functional drink.
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spelling doaj.art-5a55c2164f524e54a80f916fac7bdd092022-12-22T02:15:51ZengUniversity of RuhunaRuhuna Journal of Science1800-279X2016-12-0172646910.4038/rjs.v7i2.20Development of synbiotic beverage from beetroot juice using beneficial probiotic Lactobacillus Casei 431S.M. Gamage0M.K.S. Mihirani1O.D.A.N. Perera2H.L.D. Weerahewa3Department of Food Science and Technology, Faculty of Livestock, Fisheries and Nutrition, Wayamba University of Sri Lanka, Makandura, Gonawila (NWP), Sri LankaDepartment of Food Science and Technology, Faculty of Livestock, Fisheries and Nutrition, Wayamba University of Sri Lanka, Makandura, Gonawila (NWP), Sri LankaDepartment of Food Science and Technology, Faculty of Livestock, Fisheries and Nutrition, Wayamba University of Sri Lanka, Makandura, Gonawila (NWP), Sri LankaDepartment of Botany, The Open University, Nawala, Nugegoda, Sri LankaA beetroot beverage with Lactobacillus casei 431 as the probiotic microorganism was tested for sensory acceptability at 3 fermentation periods of 2, 4 and 6 hours at 37oC. The beverage fermented for 2 hours yielded the highest overall sensory acceptability. L. casei 431 grew well and reached nearly 108 CFU/ mL after 2 hours of fermentation at 37oC. Although the lactic culture in the fermented beetroot beverage gradually lost its viability during cold storage, viable cell count of lactic acid bacteria remained at 106–108 CFU/mL after 4 weeks of cold storage at 4oC. Titratable acidity increased significantly from 5.5±0.05 to 3.45x108 (P≤ 0.05) during storage. This study concludes that this beetroot-based synbiotic fermented beverage could be developed as a ready-to-drink product and kept for 6 weeks under refrigerated storage, meeting the standard 108–1010 CFU/mL of a functional drink.https://rjs.sljol.info/articles/10.4038/rjs.v7i2.20/galley/30/download/Functional food; Lactobacillus casei; prebiotics; probiotics; synbiotic beverageFunctional foodLactobacillus caseiprebioticsprobioticssynbiotic beverage
spellingShingle S.M. Gamage
M.K.S. Mihirani
O.D.A.N. Perera
H.L.D. Weerahewa
Development of synbiotic beverage from beetroot juice using beneficial probiotic Lactobacillus Casei 431
Ruhuna Journal of Science
Functional food; Lactobacillus casei; prebiotics; probiotics; synbiotic beverage
Functional food
Lactobacillus casei
prebiotics
probiotics
synbiotic beverage
title Development of synbiotic beverage from beetroot juice using beneficial probiotic Lactobacillus Casei 431
title_full Development of synbiotic beverage from beetroot juice using beneficial probiotic Lactobacillus Casei 431
title_fullStr Development of synbiotic beverage from beetroot juice using beneficial probiotic Lactobacillus Casei 431
title_full_unstemmed Development of synbiotic beverage from beetroot juice using beneficial probiotic Lactobacillus Casei 431
title_short Development of synbiotic beverage from beetroot juice using beneficial probiotic Lactobacillus Casei 431
title_sort development of synbiotic beverage from beetroot juice using beneficial probiotic lactobacillus casei 431
topic Functional food; Lactobacillus casei; prebiotics; probiotics; synbiotic beverage
Functional food
Lactobacillus casei
prebiotics
probiotics
synbiotic beverage
url https://rjs.sljol.info/articles/10.4038/rjs.v7i2.20/galley/30/download/
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