Bioprospecting of endophytic actinobacterium associated with Aloe ferox mill for antibacterial activity
Abstract Background The emergence of drug resistance among pathogens has resulted in renewed interest in bioprospecting for natural microbial products. Methods This study aimed to bioprospecting endophytic actinobacterium associated with Aloe ferox Mill for its antibacterial activity. Endophytic act...
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| Format: | Article |
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BMC
2022-10-01
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| Series: | BMC Complementary Medicine and Therapies |
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| Online Access: | https://doi.org/10.1186/s12906-022-03733-8 |
| _version_ | 1817984800800112640 |
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| author | Tsolanku Sidney Maliehe Melusi Mbambo Londeka Sibusisiwe Ngidi Jabulani Siyabonga Emmanuel Shandu Ofentse Jacob Pooe Peter Masoko Tlou Nelson Selepe |
| author_facet | Tsolanku Sidney Maliehe Melusi Mbambo Londeka Sibusisiwe Ngidi Jabulani Siyabonga Emmanuel Shandu Ofentse Jacob Pooe Peter Masoko Tlou Nelson Selepe |
| author_sort | Tsolanku Sidney Maliehe |
| collection | DOAJ |
| description | Abstract Background The emergence of drug resistance among pathogens has resulted in renewed interest in bioprospecting for natural microbial products. Methods This study aimed to bioprospecting endophytic actinobacterium associated with Aloe ferox Mill for its antibacterial activity. Endophytic actinomycetes were isolated from the gel of A. ferox Mill by surface sterilization technique using actinomycete isolation agar. The isolate with a promising antibacterial activity was identified using 16S rRNA sequence analysis. The minimum inhibitory concentration (MIC) of the extract was assessed by the micro-dilution method and its effect on the respiratory chain dehydrogenase (RCD) activity was ascertained by the iodonitrotetrazolium chloride (INT) assay. Fourier transform-infrared spectrophotometer (FTIR) and gas chromatography-mass spectrophotometry (GC-MS) were employed to identify functional groups and the chemical constituents, respectively. Results The actinobacterium was found to be Streptomyces olivaceus CP016795.1. Its extract displayed noteworthy antibacterial activity (MIC ≤1 mg/mL) against Staphylococcus aureus (ATCC 25925), Bacillus cereus (ATCC 10102), and Escherichia coli (ATCC 25922); and showed an inhibitory effect on the RCD activity. FTIR spectrum displayed hydroxyl, amine, and aromatic groups, and the GC–MS revealed 5-Hydroxymethylfurfural as the main constituent (19.47%). Conclusions S. olivaceus CP016795.1 can serve as a potential source of effective antibacterial compounds. |
| first_indexed | 2024-04-13T23:49:51Z |
| format | Article |
| id | doaj.art-5a55d61eda704e43b18ada166f4c40b2 |
| institution | Directory Open Access Journal |
| issn | 2662-7671 |
| language | English |
| last_indexed | 2024-04-13T23:49:51Z |
| publishDate | 2022-10-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Complementary Medicine and Therapies |
| spelling | doaj.art-5a55d61eda704e43b18ada166f4c40b22022-12-22T02:24:09ZengBMCBMC Complementary Medicine and Therapies2662-76712022-10-0122111210.1186/s12906-022-03733-8Bioprospecting of endophytic actinobacterium associated with Aloe ferox mill for antibacterial activityTsolanku Sidney Maliehe0Melusi Mbambo1Londeka Sibusisiwe Ngidi2Jabulani Siyabonga Emmanuel Shandu3Ofentse Jacob Pooe4Peter Masoko5Tlou Nelson Selepe6Department of Biochemistry and Microbiology, Faculty of Science and Agriculture, University of ZululandDepartment of Biochemistry and Microbiology, Faculty of Science and Agriculture, University of ZululandDepartment of Biochemistry and Microbiology, Faculty of Science and Agriculture, University of ZululandDepartment of Biochemistry and Microbiology, Faculty of Science and Agriculture, University of ZululandSchool of Life Science, Discipline of Biochemistry, University of KwaZulu-NatalDepartment of Biochemistry, Microbiology and Biotechnology, University of LimpopoDepartment of Water and Sanitation, University of LimpopoAbstract Background The emergence of drug resistance among pathogens has resulted in renewed interest in bioprospecting for natural microbial products. Methods This study aimed to bioprospecting endophytic actinobacterium associated with Aloe ferox Mill for its antibacterial activity. Endophytic actinomycetes were isolated from the gel of A. ferox Mill by surface sterilization technique using actinomycete isolation agar. The isolate with a promising antibacterial activity was identified using 16S rRNA sequence analysis. The minimum inhibitory concentration (MIC) of the extract was assessed by the micro-dilution method and its effect on the respiratory chain dehydrogenase (RCD) activity was ascertained by the iodonitrotetrazolium chloride (INT) assay. Fourier transform-infrared spectrophotometer (FTIR) and gas chromatography-mass spectrophotometry (GC-MS) were employed to identify functional groups and the chemical constituents, respectively. Results The actinobacterium was found to be Streptomyces olivaceus CP016795.1. Its extract displayed noteworthy antibacterial activity (MIC ≤1 mg/mL) against Staphylococcus aureus (ATCC 25925), Bacillus cereus (ATCC 10102), and Escherichia coli (ATCC 25922); and showed an inhibitory effect on the RCD activity. FTIR spectrum displayed hydroxyl, amine, and aromatic groups, and the GC–MS revealed 5-Hydroxymethylfurfural as the main constituent (19.47%). Conclusions S. olivaceus CP016795.1 can serve as a potential source of effective antibacterial compounds.https://doi.org/10.1186/s12906-022-03733-8AloeEndophytesSecondary metabolitesAntibacterial activityStreptomyces olivaceus CP016795 |
| spellingShingle | Tsolanku Sidney Maliehe Melusi Mbambo Londeka Sibusisiwe Ngidi Jabulani Siyabonga Emmanuel Shandu Ofentse Jacob Pooe Peter Masoko Tlou Nelson Selepe Bioprospecting of endophytic actinobacterium associated with Aloe ferox mill for antibacterial activity BMC Complementary Medicine and Therapies Aloe Endophytes Secondary metabolites Antibacterial activity Streptomyces olivaceus CP016795 |
| title | Bioprospecting of endophytic actinobacterium associated with Aloe ferox mill for antibacterial activity |
| title_full | Bioprospecting of endophytic actinobacterium associated with Aloe ferox mill for antibacterial activity |
| title_fullStr | Bioprospecting of endophytic actinobacterium associated with Aloe ferox mill for antibacterial activity |
| title_full_unstemmed | Bioprospecting of endophytic actinobacterium associated with Aloe ferox mill for antibacterial activity |
| title_short | Bioprospecting of endophytic actinobacterium associated with Aloe ferox mill for antibacterial activity |
| title_sort | bioprospecting of endophytic actinobacterium associated with aloe ferox mill for antibacterial activity |
| topic | Aloe Endophytes Secondary metabolites Antibacterial activity Streptomyces olivaceus CP016795 |
| url | https://doi.org/10.1186/s12906-022-03733-8 |
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