Gut microbiota composition in COVID-19 hospitalized patients with mild or severe symptoms
Background and aimCOVID-19, the infectious disease caused by SARS-CoV-2 virus that has been causing a severe pandemic worldwide for more than 2 years, is characterized by a high heterogeneity of clinical presentations and evolution and, particularly, by a varying severity of respiratory involvement....
| Main Authors: | , , , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2022-12-01
|
| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.1049215/full |
| _version_ | 1811314670668087296 |
|---|---|
| author | Antonio Mazzarelli Maria Letizia Giancola Andrea Fontana Pierluca Piselli Elena Binda Nadia Trivieri Gandino Mencarelli Luisa Marchioni Antonella Vulcano Chiara De Giuli Concetta Panebianco Annacandida Villani Massimiliano Copetti Francesco Perri Carla Fontana Emanuele Nicastri Valerio Pazienza |
| author_facet | Antonio Mazzarelli Maria Letizia Giancola Andrea Fontana Pierluca Piselli Elena Binda Nadia Trivieri Gandino Mencarelli Luisa Marchioni Antonella Vulcano Chiara De Giuli Concetta Panebianco Annacandida Villani Massimiliano Copetti Francesco Perri Carla Fontana Emanuele Nicastri Valerio Pazienza |
| author_sort | Antonio Mazzarelli |
| collection | DOAJ |
| description | Background and aimCOVID-19, the infectious disease caused by SARS-CoV-2 virus that has been causing a severe pandemic worldwide for more than 2 years, is characterized by a high heterogeneity of clinical presentations and evolution and, particularly, by a varying severity of respiratory involvement. This study aimed to analyze the diversity and taxonomic composition of the gut microbiota at hospital admission, in order to evaluate its association with COVID-19 outcome. In particular, the association between gut microbiota and a combination of several clinical covariates was analyzed in order to characterize the bacterial signature associate to mild or severe symptoms during the SARS-CoV-2 infection.Materials and methodsV3–V4 hypervariable region of 16S rRNA gene sequencing of 97 rectal swabs from a retrospective cohort of COVID-19 hospitalized patients was employed to study the gut microbiota composition. Patients were divided in two groups according to their outcome considering the respiratory supports they needed during hospital stay: (i) group “mild,” including 47 patients with a good prognosis and (ii) group “severe,” including 50 patients who experienced a more severe disease due to severe respiratory distress that required non-invasive or invasive ventilation. Identification of the clusters of bacterial population between patients with mild or severe outcome was assessed by PEnalized LOgistic Regression Analysis (PELORA).ResultsAlthough no changes for Chao1 and Shannon index were observed between the two groups a significant greater proportion of Campylobacterota and Actinobacteriota at phylum level was found in patients affected by SARS-CoV-2 infection who developed a more severe disease characterized by respiratory distress requiring invasive or non-invasive ventilation. Clusters have been identified with a useful early potential prognostic marker of the disease evolution.DiscussionMicroorganisms residing within the gut of the patients at hospital admission, were able to significantly discriminate the clinical evolution of COVID-19 patients, in particular who will develop mild or severe respiratory involvement. Our data show that patients affected by SARS-CoV-2 with mild or severe symptoms display different gut microbiota profiles which can be exploited as potential prognostic biomarkers paving also the way to new integrative therapeutic approaches. |
| first_indexed | 2024-04-13T11:16:32Z |
| format | Article |
| id | doaj.art-5a57ae835c1b4db2a8d91a8e2f17b7a1 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-13T11:16:32Z |
| publishDate | 2022-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-5a57ae835c1b4db2a8d91a8e2f17b7a12022-12-22T02:48:57ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-12-011310.3389/fmicb.2022.10492151049215Gut microbiota composition in COVID-19 hospitalized patients with mild or severe symptomsAntonio Mazzarelli0Maria Letizia Giancola1Andrea Fontana2Pierluca Piselli3Elena Binda4Nadia Trivieri5Gandino Mencarelli6Luisa Marchioni7Antonella Vulcano8Chiara De Giuli9Concetta Panebianco10Annacandida Villani11Massimiliano Copetti12Francesco Perri13Carla Fontana14Emanuele Nicastri15Valerio Pazienza16National Institute for Infectious Diseases, INMI “Lazzaro Spallanzani”, IRCCS, Rome, ItalyNational Institute for Infectious Diseases, INMI “Lazzaro Spallanzani”, IRCCS, Rome, ItalyBiostatistic Unit, Fondazione-IRCCS “Casa Sollievo della Sofferenza” Hospital, San Giovanni Rotondo, FG, ItalyNational Institute for Infectious Diseases, INMI “Lazzaro Spallanzani”, IRCCS, Rome, ItalyCancer Stem Cells Unit, Institute for Stem Cell Biologyl, Regenerative Medicine and Innovative Therapeutics (ISBReMIT), Fondazione-IRCCS “Casa Sollievo della Sofferenza” Hospital, Opera di San Pio da Pietrelcina, San Giovanni Rotondo, FG, ItalyCancer Stem Cells Unit, Institute for Stem Cell Biologyl, Regenerative Medicine and Innovative Therapeutics (ISBReMIT), Fondazione-IRCCS “Casa Sollievo della Sofferenza” Hospital, Opera di San Pio da Pietrelcina, San Giovanni Rotondo, FG, ItalyCancer Stem Cells Unit, Institute for Stem Cell Biologyl, Regenerative Medicine and Innovative Therapeutics (ISBReMIT), Fondazione-IRCCS “Casa Sollievo della Sofferenza” Hospital, Opera di San Pio da Pietrelcina, San Giovanni Rotondo, FG, ItalyNational Institute for Infectious Diseases, INMI “Lazzaro Spallanzani”, IRCCS, Rome, ItalyNational Institute for Infectious Diseases, INMI “Lazzaro Spallanzani”, IRCCS, Rome, ItalyNational Institute for Infectious Diseases, INMI “Lazzaro Spallanzani”, IRCCS, Rome, ItalyDivision of Gastroenterology, Fondazione-IRCCS “Casa Sollievo della Sofferenza” Hospital, San Giovanni Rotondo, FG, ItalyDivision of Gastroenterology, Fondazione-IRCCS “Casa Sollievo della Sofferenza” Hospital, San Giovanni Rotondo, FG, ItalyBiostatistic Unit, Fondazione-IRCCS “Casa Sollievo della Sofferenza” Hospital, San Giovanni Rotondo, FG, ItalyDivision of Gastroenterology, Fondazione-IRCCS “Casa Sollievo della Sofferenza” Hospital, San Giovanni Rotondo, FG, ItalyNational Institute for Infectious Diseases, INMI “Lazzaro Spallanzani”, IRCCS, Rome, ItalyNational Institute for Infectious Diseases, INMI “Lazzaro Spallanzani”, IRCCS, Rome, ItalyDivision of Gastroenterology, Fondazione-IRCCS “Casa Sollievo della Sofferenza” Hospital, San Giovanni Rotondo, FG, ItalyBackground and aimCOVID-19, the infectious disease caused by SARS-CoV-2 virus that has been causing a severe pandemic worldwide for more than 2 years, is characterized by a high heterogeneity of clinical presentations and evolution and, particularly, by a varying severity of respiratory involvement. This study aimed to analyze the diversity and taxonomic composition of the gut microbiota at hospital admission, in order to evaluate its association with COVID-19 outcome. In particular, the association between gut microbiota and a combination of several clinical covariates was analyzed in order to characterize the bacterial signature associate to mild or severe symptoms during the SARS-CoV-2 infection.Materials and methodsV3–V4 hypervariable region of 16S rRNA gene sequencing of 97 rectal swabs from a retrospective cohort of COVID-19 hospitalized patients was employed to study the gut microbiota composition. Patients were divided in two groups according to their outcome considering the respiratory supports they needed during hospital stay: (i) group “mild,” including 47 patients with a good prognosis and (ii) group “severe,” including 50 patients who experienced a more severe disease due to severe respiratory distress that required non-invasive or invasive ventilation. Identification of the clusters of bacterial population between patients with mild or severe outcome was assessed by PEnalized LOgistic Regression Analysis (PELORA).ResultsAlthough no changes for Chao1 and Shannon index were observed between the two groups a significant greater proportion of Campylobacterota and Actinobacteriota at phylum level was found in patients affected by SARS-CoV-2 infection who developed a more severe disease characterized by respiratory distress requiring invasive or non-invasive ventilation. Clusters have been identified with a useful early potential prognostic marker of the disease evolution.DiscussionMicroorganisms residing within the gut of the patients at hospital admission, were able to significantly discriminate the clinical evolution of COVID-19 patients, in particular who will develop mild or severe respiratory involvement. Our data show that patients affected by SARS-CoV-2 with mild or severe symptoms display different gut microbiota profiles which can be exploited as potential prognostic biomarkers paving also the way to new integrative therapeutic approaches.https://www.frontiersin.org/articles/10.3389/fmicb.2022.1049215/fullmicrobiota (16S rRNA)COVID-19biomarkersSARS-CoV-2intensive and critical care |
| spellingShingle | Antonio Mazzarelli Maria Letizia Giancola Andrea Fontana Pierluca Piselli Elena Binda Nadia Trivieri Gandino Mencarelli Luisa Marchioni Antonella Vulcano Chiara De Giuli Concetta Panebianco Annacandida Villani Massimiliano Copetti Francesco Perri Carla Fontana Emanuele Nicastri Valerio Pazienza Gut microbiota composition in COVID-19 hospitalized patients with mild or severe symptoms Frontiers in Microbiology microbiota (16S rRNA) COVID-19 biomarkers SARS-CoV-2 intensive and critical care |
| title | Gut microbiota composition in COVID-19 hospitalized patients with mild or severe symptoms |
| title_full | Gut microbiota composition in COVID-19 hospitalized patients with mild or severe symptoms |
| title_fullStr | Gut microbiota composition in COVID-19 hospitalized patients with mild or severe symptoms |
| title_full_unstemmed | Gut microbiota composition in COVID-19 hospitalized patients with mild or severe symptoms |
| title_short | Gut microbiota composition in COVID-19 hospitalized patients with mild or severe symptoms |
| title_sort | gut microbiota composition in covid 19 hospitalized patients with mild or severe symptoms |
| topic | microbiota (16S rRNA) COVID-19 biomarkers SARS-CoV-2 intensive and critical care |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.1049215/full |
| work_keys_str_mv | AT antoniomazzarelli gutmicrobiotacompositionincovid19hospitalizedpatientswithmildorseveresymptoms AT marialetiziagiancola gutmicrobiotacompositionincovid19hospitalizedpatientswithmildorseveresymptoms AT andreafontana gutmicrobiotacompositionincovid19hospitalizedpatientswithmildorseveresymptoms AT pierlucapiselli gutmicrobiotacompositionincovid19hospitalizedpatientswithmildorseveresymptoms AT elenabinda gutmicrobiotacompositionincovid19hospitalizedpatientswithmildorseveresymptoms AT nadiatrivieri gutmicrobiotacompositionincovid19hospitalizedpatientswithmildorseveresymptoms AT gandinomencarelli gutmicrobiotacompositionincovid19hospitalizedpatientswithmildorseveresymptoms AT luisamarchioni gutmicrobiotacompositionincovid19hospitalizedpatientswithmildorseveresymptoms AT antonellavulcano gutmicrobiotacompositionincovid19hospitalizedpatientswithmildorseveresymptoms AT chiaradegiuli gutmicrobiotacompositionincovid19hospitalizedpatientswithmildorseveresymptoms AT concettapanebianco gutmicrobiotacompositionincovid19hospitalizedpatientswithmildorseveresymptoms AT annacandidavillani gutmicrobiotacompositionincovid19hospitalizedpatientswithmildorseveresymptoms AT massimilianocopetti gutmicrobiotacompositionincovid19hospitalizedpatientswithmildorseveresymptoms AT francescoperri gutmicrobiotacompositionincovid19hospitalizedpatientswithmildorseveresymptoms AT carlafontana gutmicrobiotacompositionincovid19hospitalizedpatientswithmildorseveresymptoms AT emanuelenicastri gutmicrobiotacompositionincovid19hospitalizedpatientswithmildorseveresymptoms AT valeriopazienza gutmicrobiotacompositionincovid19hospitalizedpatientswithmildorseveresymptoms |