Eight-Channel AC Magnetosusceptometer of Magnetic Nanoparticles for High-Throughput and Ultra-High-Sensitivity Immunoassay
An alternating-current magnetosusceptometer of antibody-functionalized magnetic nanoparticles (MNPs) was developed for immunomagnetic reduction (IMR). A high-sensitivity, high-critical-temperature superconducting quantum interference device was used in the magnetosusceptometer. Minute levels of biom...
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MDPI AG
2018-03-01
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| Online Access: | http://www.mdpi.com/1424-8220/18/4/1043 |
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| author | Jen-Jie Chieh Wen-Chun Wei Shu-Hsien Liao Hsin-Hsein Chen Yen-Fu Lee Feng-Chun Lin Ming-Hsien Chiang Ming-Jang Chiu Herng-Er Horng Shieh-Yueh Yang |
| author_facet | Jen-Jie Chieh Wen-Chun Wei Shu-Hsien Liao Hsin-Hsein Chen Yen-Fu Lee Feng-Chun Lin Ming-Hsien Chiang Ming-Jang Chiu Herng-Er Horng Shieh-Yueh Yang |
| author_sort | Jen-Jie Chieh |
| collection | DOAJ |
| description | An alternating-current magnetosusceptometer of antibody-functionalized magnetic nanoparticles (MNPs) was developed for immunomagnetic reduction (IMR). A high-sensitivity, high-critical-temperature superconducting quantum interference device was used in the magnetosusceptometer. Minute levels of biomarkers of early-stage neurodegeneration diseases were detectable in serum, but measuring each biomarker required approximately 4 h. Hence, an eight-channel platform was developed in this study to fit minimal screening requirements for Alzheimer’s disease. Two consistent results were measured for three biomarkers, namely Aβ40, Aβ42, and tau protein, per human specimen. This paper presents the instrument configuration as well as critical characteristics, such as the low noise level variations among channels, a high signal-to-noise ratio, and the coefficient of variation for the biomarkers’ IMR values. The instrument’s ultrahigh sensitivity levels for the three biomarkers and the substantially shorter total measurement time in comparison with the previous single- and four-channels platforms were also demonstrated in this study. Thus, the eight-channel instrument may serve as a powerful tool for clinical high-throughput screening of Alzheimer’s disease. |
| first_indexed | 2024-04-11T20:55:35Z |
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| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-04-11T20:55:35Z |
| publishDate | 2018-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-5a5afeac26f34cfba1b2a20e97f19ea92022-12-22T04:03:41ZengMDPI AGSensors1424-82202018-03-01184104310.3390/s18041043s18041043Eight-Channel AC Magnetosusceptometer of Magnetic Nanoparticles for High-Throughput and Ultra-High-Sensitivity ImmunoassayJen-Jie Chieh0Wen-Chun Wei1Shu-Hsien Liao2Hsin-Hsein Chen3Yen-Fu Lee4Feng-Chun Lin5Ming-Hsien Chiang6Ming-Jang Chiu7Herng-Er Horng8Shieh-Yueh Yang9Institute of Electro-Optical Science and Technology, National Taiwan Normal University, Taipei 116, TaiwanInstitute of Electro-Optical Science and Technology, National Taiwan Normal University, Taipei 116, TaiwanInstitute of Electro-Optical Science and Technology, National Taiwan Normal University, Taipei 116, TaiwanMagQu Co., Ltd., New Taipei 231, TaiwanMagQu Co., Ltd., New Taipei 231, TaiwanMagQu Co., Ltd., New Taipei 231, TaiwanInstitute of Electro-Optical Science and Technology, National Taiwan Normal University, Taipei 116, TaiwanDepartments of Neurology, National Taiwan University Hospital, Taipei 100, TaiwanInstitute of Electro-Optical Science and Technology, National Taiwan Normal University, Taipei 116, TaiwanMagQu Co., Ltd., New Taipei 231, TaiwanAn alternating-current magnetosusceptometer of antibody-functionalized magnetic nanoparticles (MNPs) was developed for immunomagnetic reduction (IMR). A high-sensitivity, high-critical-temperature superconducting quantum interference device was used in the magnetosusceptometer. Minute levels of biomarkers of early-stage neurodegeneration diseases were detectable in serum, but measuring each biomarker required approximately 4 h. Hence, an eight-channel platform was developed in this study to fit minimal screening requirements for Alzheimer’s disease. Two consistent results were measured for three biomarkers, namely Aβ40, Aβ42, and tau protein, per human specimen. This paper presents the instrument configuration as well as critical characteristics, such as the low noise level variations among channels, a high signal-to-noise ratio, and the coefficient of variation for the biomarkers’ IMR values. The instrument’s ultrahigh sensitivity levels for the three biomarkers and the substantially shorter total measurement time in comparison with the previous single- and four-channels platforms were also demonstrated in this study. Thus, the eight-channel instrument may serve as a powerful tool for clinical high-throughput screening of Alzheimer’s disease.http://www.mdpi.com/1424-8220/18/4/1043superconducting quantum interference devicemagnetic nanoparticleimmunoassay |
| spellingShingle | Jen-Jie Chieh Wen-Chun Wei Shu-Hsien Liao Hsin-Hsein Chen Yen-Fu Lee Feng-Chun Lin Ming-Hsien Chiang Ming-Jang Chiu Herng-Er Horng Shieh-Yueh Yang Eight-Channel AC Magnetosusceptometer of Magnetic Nanoparticles for High-Throughput and Ultra-High-Sensitivity Immunoassay Sensors superconducting quantum interference device magnetic nanoparticle immunoassay |
| title | Eight-Channel AC Magnetosusceptometer of Magnetic Nanoparticles for High-Throughput and Ultra-High-Sensitivity Immunoassay |
| title_full | Eight-Channel AC Magnetosusceptometer of Magnetic Nanoparticles for High-Throughput and Ultra-High-Sensitivity Immunoassay |
| title_fullStr | Eight-Channel AC Magnetosusceptometer of Magnetic Nanoparticles for High-Throughput and Ultra-High-Sensitivity Immunoassay |
| title_full_unstemmed | Eight-Channel AC Magnetosusceptometer of Magnetic Nanoparticles for High-Throughput and Ultra-High-Sensitivity Immunoassay |
| title_short | Eight-Channel AC Magnetosusceptometer of Magnetic Nanoparticles for High-Throughput and Ultra-High-Sensitivity Immunoassay |
| title_sort | eight channel ac magnetosusceptometer of magnetic nanoparticles for high throughput and ultra high sensitivity immunoassay |
| topic | superconducting quantum interference device magnetic nanoparticle immunoassay |
| url | http://www.mdpi.com/1424-8220/18/4/1043 |
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