Association of Methylenetetrahydrofolate Reductase rs1801133 Genetic Variants with Type 2 Diabetes Mellitus and Diabetic Nephropathy
Background: Type 2 diabetes mellitus (T2DM) is a complex metabolic disease with a genetic predisposition. Methylenetetrahydrofolatereductase (MTHFR) gene is one of the candidate genes associated with T2DM and diabetic nephropathy (DN). This research was carried out to determine the frequency of the...
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| Format: | Article |
| Language: | English |
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Universitas Indonesia
2022-08-01
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| Series: | Makara Journal of Health Research |
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| Online Access: | https://scholarhub.ui.ac.id/mjhr/vol26/iss2/1/ |
| _version_ | 1797905187624452096 |
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| author | Aysegul Bayramoglu Gokhan Bayramoglu Halil Ibrahım Guler Nezaket Coban Mustafa Çagatay Korkmaz |
| author_facet | Aysegul Bayramoglu Gokhan Bayramoglu Halil Ibrahım Guler Nezaket Coban Mustafa Çagatay Korkmaz |
| author_sort | Aysegul Bayramoglu |
| collection | DOAJ |
| description | Background: Type 2 diabetes mellitus (T2DM) is a complex metabolic disease with a genetic predisposition. Methylenetetrahydrofolatereductase (MTHFR) gene is one of the candidate genes associated with T2DM and diabetic nephropathy (DN). This research was carried out to determine the frequency of the C677T polymorphism (rs1801133) of the MTHFR gene and examine the role of rs1801133 polymorphism in T2DM and DN development.
Methods: DNA was obtained from peripheral blood samples (273 samples) using a DNA isolation kit. MTHFR rs1801133 polymorphism was determined using polymerase chain reaction (PCR), restriction fragment length polymorphism (RFLP), and electrophoresis. PCR products were cut by restriction enzyme HiNF I and analyzed by 2% agarose gel electrophoresis. The results were statistically analyzed.
Results: Although MTHFR rs1801133 genotype frequencies showed statistically significant differences between the control and T2DM patient groups (p = 0.001), no statistically significant difference was found between individuals with and without DN.
Conclusions: MTHFR gene rs1801133 polymorphism is related to T2DM but not to DN. CT and TT genotypes can be accepted as genetic markers. |
| first_indexed | 2024-04-10T10:02:14Z |
| format | Article |
| id | doaj.art-5a5f52e0c20f4867ae7ddcf497f3e03d |
| institution | Directory Open Access Journal |
| issn | 2356-3664 2356-3656 |
| language | English |
| last_indexed | 2024-04-10T10:02:14Z |
| publishDate | 2022-08-01 |
| publisher | Universitas Indonesia |
| record_format | Article |
| series | Makara Journal of Health Research |
| spelling | doaj.art-5a5f52e0c20f4867ae7ddcf497f3e03d2023-02-16T07:13:16ZengUniversitas IndonesiaMakara Journal of Health Research2356-36642356-36562022-08-01262748010.7454/msk.v26i2.1352Association of Methylenetetrahydrofolate Reductase rs1801133 Genetic Variants with Type 2 Diabetes Mellitus and Diabetic NephropathyAysegul Bayramoglu 0https://orcid.org/0000-0001-8567-807XGokhan Bayramoglu1https://orcid.org/0000-0003-2779-6478Halil Ibrahım Guler2https://orcid.org/0000-0002-7261-6790Nezaket Coban3https://orcid.org/0000-0002-1893-4229Mustafa Çagatay Korkmaz4https://orcid.org/0000-0003-3302-0705Department of Nutrition and Dietetics, Faculty of Health Sciences, Kutahya Health Sciences University, Kutahya 43100, TurkeyDepartment of Occupational Health and Safety, Faculty of Health Sciences, Artvin Coruh University, Artvin 08000, TurkeyFDepartment of Molecular Biology and Genetics, Faculty of Science, Karadeniz Technical University, Trabzon 61080, TurkeyDepartment of Molecular Biology and Genetics, Institute of Science, Artvin Coruh University, Artvin 08000, TurkeyDepartment of Measurement and Evaluation, Faculty of Education, Artvin Coruh University, Artvin 08000, TurkeyBackground: Type 2 diabetes mellitus (T2DM) is a complex metabolic disease with a genetic predisposition. Methylenetetrahydrofolatereductase (MTHFR) gene is one of the candidate genes associated with T2DM and diabetic nephropathy (DN). This research was carried out to determine the frequency of the C677T polymorphism (rs1801133) of the MTHFR gene and examine the role of rs1801133 polymorphism in T2DM and DN development. Methods: DNA was obtained from peripheral blood samples (273 samples) using a DNA isolation kit. MTHFR rs1801133 polymorphism was determined using polymerase chain reaction (PCR), restriction fragment length polymorphism (RFLP), and electrophoresis. PCR products were cut by restriction enzyme HiNF I and analyzed by 2% agarose gel electrophoresis. The results were statistically analyzed. Results: Although MTHFR rs1801133 genotype frequencies showed statistically significant differences between the control and T2DM patient groups (p = 0.001), no statistically significant difference was found between individuals with and without DN. Conclusions: MTHFR gene rs1801133 polymorphism is related to T2DM but not to DN. CT and TT genotypes can be accepted as genetic markers.https://scholarhub.ui.ac.id/mjhr/vol26/iss2/1/: diabetic nephropathygenetic variationmethylenetetrahydrofolate reductaserestriction fragment length polymorphismtype 2 diabetes mellitus |
| spellingShingle | Aysegul Bayramoglu Gokhan Bayramoglu Halil Ibrahım Guler Nezaket Coban Mustafa Çagatay Korkmaz Association of Methylenetetrahydrofolate Reductase rs1801133 Genetic Variants with Type 2 Diabetes Mellitus and Diabetic Nephropathy Makara Journal of Health Research : diabetic nephropathy genetic variation methylenetetrahydrofolate reductase restriction fragment length polymorphism type 2 diabetes mellitus |
| title | Association of Methylenetetrahydrofolate Reductase rs1801133 Genetic Variants with Type 2 Diabetes Mellitus and Diabetic Nephropathy |
| title_full | Association of Methylenetetrahydrofolate Reductase rs1801133 Genetic Variants with Type 2 Diabetes Mellitus and Diabetic Nephropathy |
| title_fullStr | Association of Methylenetetrahydrofolate Reductase rs1801133 Genetic Variants with Type 2 Diabetes Mellitus and Diabetic Nephropathy |
| title_full_unstemmed | Association of Methylenetetrahydrofolate Reductase rs1801133 Genetic Variants with Type 2 Diabetes Mellitus and Diabetic Nephropathy |
| title_short | Association of Methylenetetrahydrofolate Reductase rs1801133 Genetic Variants with Type 2 Diabetes Mellitus and Diabetic Nephropathy |
| title_sort | association of methylenetetrahydrofolate reductase rs1801133 genetic variants with type 2 diabetes mellitus and diabetic nephropathy |
| topic | : diabetic nephropathy genetic variation methylenetetrahydrofolate reductase restriction fragment length polymorphism type 2 diabetes mellitus |
| url | https://scholarhub.ui.ac.id/mjhr/vol26/iss2/1/ |
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