Scale-Up Production of Type O and A Foot-and-Mouth Disease Bivalent Vaccine and Its Protective Efficacy in Pigs
South Korea has experienced FMD outbreaks almost every year since 2014. Therefore, a novel local vaccine that can cover various topotypes of viruses is required. Two virus strains, O/Boeun/SKR/2017 and A/Yeoncheon/SKR/2017, were cultured up to the pilot scale based on the optimized conditions set up...
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| Format: | Article |
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MDPI AG
2021-06-01
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| Series: | Vaccines |
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| Online Access: | https://www.mdpi.com/2076-393X/9/6/586 |
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| author | Sang-Hyun Park Seo-Yong Lee Jae-Seok Kim Ah-Young Kim Sun-Young Park Ji-Hye Lee Mijung Lee Hyejin Kim Sim-In Lee Na-Young Kang Jung-Won Park Su-Mi Kim Jong-Hyeon Park Young-Joon Ko |
| author_facet | Sang-Hyun Park Seo-Yong Lee Jae-Seok Kim Ah-Young Kim Sun-Young Park Ji-Hye Lee Mijung Lee Hyejin Kim Sim-In Lee Na-Young Kang Jung-Won Park Su-Mi Kim Jong-Hyeon Park Young-Joon Ko |
| author_sort | Sang-Hyun Park |
| collection | DOAJ |
| description | South Korea has experienced FMD outbreaks almost every year since 2014. Therefore, a novel local vaccine that can cover various topotypes of viruses is required. Two virus strains, O/Boeun/SKR/2017 and A/Yeoncheon/SKR/2017, were cultured up to the pilot scale based on the optimized conditions set up on the flask scale. FMDV particles (146S) of 2 µg/mL or more were obtained from the virus culture supernatant using a 100 L bioreactor. The viruses were fully inactivated using binary ethylenimine within 16 h through two inactivation cycles and mixed with an adjuvant into a bivalent vaccine (types O and A) consisting of 15 µg viruses per strain. The experimental bivalent vaccine showed a broad spectrum of high neutralizing antibody titers against heterologous viruses, including type O Cathay strain and type A Asia topotypes, except for GVII. The 50% protective dose was determined as 12.5 for O/Boeun/SKR/2017 and 15.6 for A/Yeoncheon/SKR/2017. Collectively, we expect that the bivalent vaccine could protect against FMDV types O and A circulating in South Korea and neighboring countries. To our knowledge, this is the first report demonstrating that the vaccine strains could be successfully scaled-up to a 100 L bioreactor, with the determination of its protective efficacy in pigs. |
| first_indexed | 2024-03-10T10:47:46Z |
| format | Article |
| id | doaj.art-5a602b82dba64c09b2a9a561cdcd74be |
| institution | Directory Open Access Journal |
| issn | 2076-393X |
| language | English |
| last_indexed | 2024-03-10T10:47:46Z |
| publishDate | 2021-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Vaccines |
| spelling | doaj.art-5a602b82dba64c09b2a9a561cdcd74be2023-11-21T22:29:38ZengMDPI AGVaccines2076-393X2021-06-019658610.3390/vaccines9060586Scale-Up Production of Type O and A Foot-and-Mouth Disease Bivalent Vaccine and Its Protective Efficacy in PigsSang-Hyun Park0Seo-Yong Lee1Jae-Seok Kim2Ah-Young Kim3Sun-Young Park4Ji-Hye Lee5Mijung Lee6Hyejin Kim7Sim-In Lee8Na-Young Kang9Jung-Won Park10Su-Mi Kim11Jong-Hyeon Park12Young-Joon Ko13Animal and Plant Quarantine Agency, 177 Hyeoksin-8-ro, Gimcheon-si 39660, Gyeongsangbuk-do, KoreaResearch Unit, FVC Vaccine Company, #521, 5, Hyeoksin 8-ro, Gimcheon-si 39660, Gyeongsangbuk-do, KoreaAnimal and Plant Quarantine Agency, 177 Hyeoksin-8-ro, Gimcheon-si 39660, Gyeongsangbuk-do, KoreaAnimal and Plant Quarantine Agency, 177 Hyeoksin-8-ro, Gimcheon-si 39660, Gyeongsangbuk-do, KoreaAnimal and Plant Quarantine Agency, 177 Hyeoksin-8-ro, Gimcheon-si 39660, Gyeongsangbuk-do, KoreaResearch Unit, FVC Vaccine Company, #521, 5, Hyeoksin 8-ro, Gimcheon-si 39660, Gyeongsangbuk-do, KoreaResearch Unit, FVC Vaccine Company, #521, 5, Hyeoksin 8-ro, Gimcheon-si 39660, Gyeongsangbuk-do, KoreaAnimal and Plant Quarantine Agency, 177 Hyeoksin-8-ro, Gimcheon-si 39660, Gyeongsangbuk-do, KoreaAnimal and Plant Quarantine Agency, 177 Hyeoksin-8-ro, Gimcheon-si 39660, Gyeongsangbuk-do, KoreaAnimal and Plant Quarantine Agency, 177 Hyeoksin-8-ro, Gimcheon-si 39660, Gyeongsangbuk-do, KoreaAnimal and Plant Quarantine Agency, 177 Hyeoksin-8-ro, Gimcheon-si 39660, Gyeongsangbuk-do, KoreaAnimal and Plant Quarantine Agency, 177 Hyeoksin-8-ro, Gimcheon-si 39660, Gyeongsangbuk-do, KoreaAnimal and Plant Quarantine Agency, 177 Hyeoksin-8-ro, Gimcheon-si 39660, Gyeongsangbuk-do, KoreaAnimal and Plant Quarantine Agency, 177 Hyeoksin-8-ro, Gimcheon-si 39660, Gyeongsangbuk-do, KoreaSouth Korea has experienced FMD outbreaks almost every year since 2014. Therefore, a novel local vaccine that can cover various topotypes of viruses is required. Two virus strains, O/Boeun/SKR/2017 and A/Yeoncheon/SKR/2017, were cultured up to the pilot scale based on the optimized conditions set up on the flask scale. FMDV particles (146S) of 2 µg/mL or more were obtained from the virus culture supernatant using a 100 L bioreactor. The viruses were fully inactivated using binary ethylenimine within 16 h through two inactivation cycles and mixed with an adjuvant into a bivalent vaccine (types O and A) consisting of 15 µg viruses per strain. The experimental bivalent vaccine showed a broad spectrum of high neutralizing antibody titers against heterologous viruses, including type O Cathay strain and type A Asia topotypes, except for GVII. The 50% protective dose was determined as 12.5 for O/Boeun/SKR/2017 and 15.6 for A/Yeoncheon/SKR/2017. Collectively, we expect that the bivalent vaccine could protect against FMDV types O and A circulating in South Korea and neighboring countries. To our knowledge, this is the first report demonstrating that the vaccine strains could be successfully scaled-up to a 100 L bioreactor, with the determination of its protective efficacy in pigs.https://www.mdpi.com/2076-393X/9/6/586FMDfoot-and-mouth diseasescale-upbivalent vaccineprotective efficacy |
| spellingShingle | Sang-Hyun Park Seo-Yong Lee Jae-Seok Kim Ah-Young Kim Sun-Young Park Ji-Hye Lee Mijung Lee Hyejin Kim Sim-In Lee Na-Young Kang Jung-Won Park Su-Mi Kim Jong-Hyeon Park Young-Joon Ko Scale-Up Production of Type O and A Foot-and-Mouth Disease Bivalent Vaccine and Its Protective Efficacy in Pigs Vaccines FMD foot-and-mouth disease scale-up bivalent vaccine protective efficacy |
| title | Scale-Up Production of Type O and A Foot-and-Mouth Disease Bivalent Vaccine and Its Protective Efficacy in Pigs |
| title_full | Scale-Up Production of Type O and A Foot-and-Mouth Disease Bivalent Vaccine and Its Protective Efficacy in Pigs |
| title_fullStr | Scale-Up Production of Type O and A Foot-and-Mouth Disease Bivalent Vaccine and Its Protective Efficacy in Pigs |
| title_full_unstemmed | Scale-Up Production of Type O and A Foot-and-Mouth Disease Bivalent Vaccine and Its Protective Efficacy in Pigs |
| title_short | Scale-Up Production of Type O and A Foot-and-Mouth Disease Bivalent Vaccine and Its Protective Efficacy in Pigs |
| title_sort | scale up production of type o and a foot and mouth disease bivalent vaccine and its protective efficacy in pigs |
| topic | FMD foot-and-mouth disease scale-up bivalent vaccine protective efficacy |
| url | https://www.mdpi.com/2076-393X/9/6/586 |
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