Sodium Arsenite Caused Mineralization Impairment in Rat Bone Marrow Mesenchymal Stem Cells Differentiating to Osteoblasts
Background: Sodium arsenite (SA) recently has been recommended to be used in malignancy therapy. Our studies showed, SA in short and long period of treatment caused reduction of rats Bone Marrow Mesenchymal Stem Cells (MSCs) viability and induced caspase dependent apoptosis. The aim of this study wa...
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| Format: | Article |
| Language: | English |
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Arak University of Medical Sciences
2012-05-01
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| Series: | Iranian Journal of Toxicology |
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| Online Access: | http://ijt.arakmu.ac.ir/browse.php?a_id=115&slc_lang=en&sid=1&ftxt=1 |
| _version_ | 1811298967790551040 |
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| author | Mohammad Hossein Abnosi Zahra Jafari Yazdi |
| author_facet | Mohammad Hossein Abnosi Zahra Jafari Yazdi |
| author_sort | Mohammad Hossein Abnosi |
| collection | DOAJ |
| description | Background: Sodium arsenite (SA) recently has been recommended to be used in malignancy therapy. Our studies showed, SA in short and long period of treatment caused reduction of rats Bone Marrow Mesenchymal Stem Cells (MSCs) viability and induced caspase dependent apoptosis. The aim of this study was to investigate the effect of SA on osteogenic differentiation of MSCs.
Methods: MSCs were extracted and expanded to third passage, then cultured in DMEM supplemented with osteogenic media in presence of 1 and 25nM of SA for 21 days. The viability and the level of mineralization were determined using MTT assay and alizarin red respectively. In addition morphology and nuclear diameter of the cells were studied with the help of fluorescent dye. Furthermore, calcium content and alkalinphosphatase activity also were estimated using commercial kit. Data was statistically analyzed and the P<0.05 was taken as the level of significant.
Results: The viability and mineralization of the cells treated with SA reduced significantly (P<0.05) after tenth day in compare with control. Also, chromatin condensation, reduction of nuclei diameter and cytoplasm shrinkage were observed in the cell treated with 1 and 25 nM concentrations. The calcium and alkalinphosphatase activity of the cells decreased significantly with 1 and 25 nM concentrations of SA when compared with control.
Conclusion: Adverse effect of SA was observed on osteogenic differentiation of MSCs at 1 and 25 nM due to disruption of mineralization. We strongly suggest more investigation to be run on this chemical with respect to the therapy of the malignant patients. |
| first_indexed | 2024-04-13T06:28:05Z |
| format | Article |
| id | doaj.art-5a603724bbc1402fa94f8b015d2469ec |
| institution | Directory Open Access Journal |
| issn | 2008-2967 2251-9459 |
| language | English |
| last_indexed | 2024-04-13T06:28:05Z |
| publishDate | 2012-05-01 |
| publisher | Arak University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Toxicology |
| spelling | doaj.art-5a603724bbc1402fa94f8b015d2469ec2022-12-22T02:58:17ZengArak University of Medical SciencesIranian Journal of Toxicology2008-29672251-94592012-05-01616577587Sodium Arsenite Caused Mineralization Impairment in Rat Bone Marrow Mesenchymal Stem Cells Differentiating to OsteoblastsMohammad Hossein Abnosi 0Zahra Jafari Yazdi1Department of Biology, Arak University of Medical Sciences, Arak, Iran.*Corresponding Author E-mail: abnosi2002@yahoo.comDepartment of Biology, Arak University of Medical Sciences, Arak, Iran.Background: Sodium arsenite (SA) recently has been recommended to be used in malignancy therapy. Our studies showed, SA in short and long period of treatment caused reduction of rats Bone Marrow Mesenchymal Stem Cells (MSCs) viability and induced caspase dependent apoptosis. The aim of this study was to investigate the effect of SA on osteogenic differentiation of MSCs. Methods: MSCs were extracted and expanded to third passage, then cultured in DMEM supplemented with osteogenic media in presence of 1 and 25nM of SA for 21 days. The viability and the level of mineralization were determined using MTT assay and alizarin red respectively. In addition morphology and nuclear diameter of the cells were studied with the help of fluorescent dye. Furthermore, calcium content and alkalinphosphatase activity also were estimated using commercial kit. Data was statistically analyzed and the P<0.05 was taken as the level of significant. Results: The viability and mineralization of the cells treated with SA reduced significantly (P<0.05) after tenth day in compare with control. Also, chromatin condensation, reduction of nuclei diameter and cytoplasm shrinkage were observed in the cell treated with 1 and 25 nM concentrations. The calcium and alkalinphosphatase activity of the cells decreased significantly with 1 and 25 nM concentrations of SA when compared with control. Conclusion: Adverse effect of SA was observed on osteogenic differentiation of MSCs at 1 and 25 nM due to disruption of mineralization. We strongly suggest more investigation to be run on this chemical with respect to the therapy of the malignant patients.http://ijt.arakmu.ac.ir/browse.php?a_id=115&slc_lang=en&sid=1&ftxt=1Mesenchymal Stem CellMineralizationOsteogenic DifferentiationSodium Arsenite |
| spellingShingle | Mohammad Hossein Abnosi Zahra Jafari Yazdi Sodium Arsenite Caused Mineralization Impairment in Rat Bone Marrow Mesenchymal Stem Cells Differentiating to Osteoblasts Iranian Journal of Toxicology Mesenchymal Stem Cell Mineralization Osteogenic Differentiation Sodium Arsenite |
| title | Sodium Arsenite Caused Mineralization Impairment in Rat Bone Marrow Mesenchymal Stem Cells Differentiating to Osteoblasts |
| title_full | Sodium Arsenite Caused Mineralization Impairment in Rat Bone Marrow Mesenchymal Stem Cells Differentiating to Osteoblasts |
| title_fullStr | Sodium Arsenite Caused Mineralization Impairment in Rat Bone Marrow Mesenchymal Stem Cells Differentiating to Osteoblasts |
| title_full_unstemmed | Sodium Arsenite Caused Mineralization Impairment in Rat Bone Marrow Mesenchymal Stem Cells Differentiating to Osteoblasts |
| title_short | Sodium Arsenite Caused Mineralization Impairment in Rat Bone Marrow Mesenchymal Stem Cells Differentiating to Osteoblasts |
| title_sort | sodium arsenite caused mineralization impairment in rat bone marrow mesenchymal stem cells differentiating to osteoblasts |
| topic | Mesenchymal Stem Cell Mineralization Osteogenic Differentiation Sodium Arsenite |
| url | http://ijt.arakmu.ac.ir/browse.php?a_id=115&slc_lang=en&sid=1&ftxt=1 |
| work_keys_str_mv | AT mohammadhosseinabnosi sodiumarsenitecausedmineralizationimpairmentinratbonemarrowmesenchymalstemcellsdifferentiatingtoosteoblasts AT zahrajafariyazdi sodiumarsenitecausedmineralizationimpairmentinratbonemarrowmesenchymalstemcellsdifferentiatingtoosteoblasts |