A reference proteomic database of Lactobacillus plantarum CMCC-P0002.
Lactobacillus plantarum is a widespread probiotic bacteria found in many fermented food products. In this study, the whole-cell proteins and secretory proteins of L. plantarum were separated by two-dimensional electrophoresis method. A total of 434 proteins were identified by tandem mass spectrometr...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2011-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3187783?pdf=render |
| _version_ | 1828516558130905088 |
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| author | Li Zhu Wei Hu Datao Liu Wanhong Tian Gang Yu Xiankai Liu Jie Wang Erling Feng Xuemin Zhang Bei Chen Ming Zeng Hengliang Wang |
| author_facet | Li Zhu Wei Hu Datao Liu Wanhong Tian Gang Yu Xiankai Liu Jie Wang Erling Feng Xuemin Zhang Bei Chen Ming Zeng Hengliang Wang |
| author_sort | Li Zhu |
| collection | DOAJ |
| description | Lactobacillus plantarum is a widespread probiotic bacteria found in many fermented food products. In this study, the whole-cell proteins and secretory proteins of L. plantarum were separated by two-dimensional electrophoresis method. A total of 434 proteins were identified by tandem mass spectrometry, including a plasmid-encoded hypothetical protein pLP9000_05. The information of first 20 highest abundance proteins was listed for the further genetic manipulation of L. plantarum, such as construction of high-level expressions system. Furthermore, the first interaction map of L. plantarum was established by Blue-Native/SDS-PAGE technique. A heterodimeric complex composed of maltose phosphorylase Map3 and Map2, and two homodimeric complexes composed of Map3 and Map2 respectively, were identified at the same time, indicating the important roles of these proteins. These findings provided valuable information for the further proteomic researches of L. plantarum. |
| first_indexed | 2024-12-11T18:29:42Z |
| format | Article |
| id | doaj.art-5a62278bf2bc4d20a5da319ff72972d4 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-11T18:29:42Z |
| publishDate | 2011-01-01 |
| publisher | Public Library of Science (PLoS) |
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| series | PLoS ONE |
| spelling | doaj.art-5a62278bf2bc4d20a5da319ff72972d42022-12-22T00:54:57ZengPublic Library of Science (PLoS)PLoS ONE1932-62032011-01-01610e2559610.1371/journal.pone.0025596A reference proteomic database of Lactobacillus plantarum CMCC-P0002.Li ZhuWei HuDatao LiuWanhong TianGang YuXiankai LiuJie WangErling FengXuemin ZhangBei ChenMing ZengHengliang WangLactobacillus plantarum is a widespread probiotic bacteria found in many fermented food products. In this study, the whole-cell proteins and secretory proteins of L. plantarum were separated by two-dimensional electrophoresis method. A total of 434 proteins were identified by tandem mass spectrometry, including a plasmid-encoded hypothetical protein pLP9000_05. The information of first 20 highest abundance proteins was listed for the further genetic manipulation of L. plantarum, such as construction of high-level expressions system. Furthermore, the first interaction map of L. plantarum was established by Blue-Native/SDS-PAGE technique. A heterodimeric complex composed of maltose phosphorylase Map3 and Map2, and two homodimeric complexes composed of Map3 and Map2 respectively, were identified at the same time, indicating the important roles of these proteins. These findings provided valuable information for the further proteomic researches of L. plantarum.http://europepmc.org/articles/PMC3187783?pdf=render |
| spellingShingle | Li Zhu Wei Hu Datao Liu Wanhong Tian Gang Yu Xiankai Liu Jie Wang Erling Feng Xuemin Zhang Bei Chen Ming Zeng Hengliang Wang A reference proteomic database of Lactobacillus plantarum CMCC-P0002. PLoS ONE |
| title | A reference proteomic database of Lactobacillus plantarum CMCC-P0002. |
| title_full | A reference proteomic database of Lactobacillus plantarum CMCC-P0002. |
| title_fullStr | A reference proteomic database of Lactobacillus plantarum CMCC-P0002. |
| title_full_unstemmed | A reference proteomic database of Lactobacillus plantarum CMCC-P0002. |
| title_short | A reference proteomic database of Lactobacillus plantarum CMCC-P0002. |
| title_sort | reference proteomic database of lactobacillus plantarum cmcc p0002 |
| url | http://europepmc.org/articles/PMC3187783?pdf=render |
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