RETRACTED ARTICLE: Fibulin-3 promotes osteosarcoma invasion and metastasis by inducing epithelial to mesenchymal transition and activating the Wnt/β-catenin signaling pathway
Abstract This study explored the role of fibulin-3 in osteosarcoma progression and the possible signaling pathway involved. Fibulin-3 mRNA and protein expression in normal tissue, benign fibrous dysplasia, osteosarcoma, osteosarcoma cell lines (HOS and U-2OS), the normal osteoblastic cell line hFOB,...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2017-07-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-017-06353-2 |
| _version_ | 1797774153532571648 |
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| author | Songgang Wang Dong Zhang Shasha Han Peng Gao Changying Liu Jianmin Li Xin Pan |
| author_facet | Songgang Wang Dong Zhang Shasha Han Peng Gao Changying Liu Jianmin Li Xin Pan |
| author_sort | Songgang Wang |
| collection | DOAJ |
| description | Abstract This study explored the role of fibulin-3 in osteosarcoma progression and the possible signaling pathway involved. Fibulin-3 mRNA and protein expression in normal tissue, benign fibrous dysplasia, osteosarcoma, osteosarcoma cell lines (HOS and U-2OS), the normal osteoblastic cell line hFOB, and different invasive subclones was evaluated by immunohistochemistry (IHC) or immunocytochemistry (ICC) and real time reverse transcriptase-polymerase chain reaction (real time qRT-PCR). To assess the role of fibulin-3 in the invasion and metastasis of osteosarcoma cells, lentiviral vectors with fibulin-3 small hairpin RNA (shRNA) and pLVX-fibulin-3 were constructed and used to infect the highly invasive and low invasive subclones. The effects of fibulin-3 knockdown and upregulation on the biological behavior of osteosarcoma cells were investigated by functional in vitro and in vivo assays. The results revealed that fibulin-3 expression was upregulated in osteosarcoma, and was positively correlated with low differentiation, lymph node metastasis, and poor prognosis. Fibulin-3 could promote osteosarcoma cell invasion and metastasis by inducing EMT and activating the Wnt/β-catenin signaling pathway. Collectively, our findings demonstrate that fibulin-3 is a promoter of osteosarcoma development and progression, and suggest a novel therapeutic target for future studies. |
| first_indexed | 2024-03-12T22:16:48Z |
| format | Article |
| id | doaj.art-5a62527b0fb1473b969f39368b77c320 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-12T22:16:48Z |
| publishDate | 2017-07-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-5a62527b0fb1473b969f39368b77c3202023-07-23T11:15:29ZengNature PortfolioScientific Reports2045-23222017-07-017111710.1038/s41598-017-06353-2RETRACTED ARTICLE: Fibulin-3 promotes osteosarcoma invasion and metastasis by inducing epithelial to mesenchymal transition and activating the Wnt/β-catenin signaling pathwaySonggang Wang0Dong Zhang1Shasha Han2Peng Gao3Changying Liu4Jianmin Li5Xin Pan6Department of orthopedics, Qilu Hospital, Shandong UniversityDepartment of orthopedics, Qilu Hospital, Shandong UniversityDepartment of orthopedics, Qilu Hospital, Shandong UniversityDepartment of orthopedics, People’s Hospital of zhangqiuDepartment of orthopedics, People’s Hospital of YinanDepartment of orthopedics, Qilu Hospital, Shandong UniversityDepartment of orthopedics, Qilu Hospital, Shandong UniversityAbstract This study explored the role of fibulin-3 in osteosarcoma progression and the possible signaling pathway involved. Fibulin-3 mRNA and protein expression in normal tissue, benign fibrous dysplasia, osteosarcoma, osteosarcoma cell lines (HOS and U-2OS), the normal osteoblastic cell line hFOB, and different invasive subclones was evaluated by immunohistochemistry (IHC) or immunocytochemistry (ICC) and real time reverse transcriptase-polymerase chain reaction (real time qRT-PCR). To assess the role of fibulin-3 in the invasion and metastasis of osteosarcoma cells, lentiviral vectors with fibulin-3 small hairpin RNA (shRNA) and pLVX-fibulin-3 were constructed and used to infect the highly invasive and low invasive subclones. The effects of fibulin-3 knockdown and upregulation on the biological behavior of osteosarcoma cells were investigated by functional in vitro and in vivo assays. The results revealed that fibulin-3 expression was upregulated in osteosarcoma, and was positively correlated with low differentiation, lymph node metastasis, and poor prognosis. Fibulin-3 could promote osteosarcoma cell invasion and metastasis by inducing EMT and activating the Wnt/β-catenin signaling pathway. Collectively, our findings demonstrate that fibulin-3 is a promoter of osteosarcoma development and progression, and suggest a novel therapeutic target for future studies.https://doi.org/10.1038/s41598-017-06353-2 |
| spellingShingle | Songgang Wang Dong Zhang Shasha Han Peng Gao Changying Liu Jianmin Li Xin Pan RETRACTED ARTICLE: Fibulin-3 promotes osteosarcoma invasion and metastasis by inducing epithelial to mesenchymal transition and activating the Wnt/β-catenin signaling pathway Scientific Reports |
| title | RETRACTED ARTICLE: Fibulin-3 promotes osteosarcoma invasion and metastasis by inducing epithelial to mesenchymal transition and activating the Wnt/β-catenin signaling pathway |
| title_full | RETRACTED ARTICLE: Fibulin-3 promotes osteosarcoma invasion and metastasis by inducing epithelial to mesenchymal transition and activating the Wnt/β-catenin signaling pathway |
| title_fullStr | RETRACTED ARTICLE: Fibulin-3 promotes osteosarcoma invasion and metastasis by inducing epithelial to mesenchymal transition and activating the Wnt/β-catenin signaling pathway |
| title_full_unstemmed | RETRACTED ARTICLE: Fibulin-3 promotes osteosarcoma invasion and metastasis by inducing epithelial to mesenchymal transition and activating the Wnt/β-catenin signaling pathway |
| title_short | RETRACTED ARTICLE: Fibulin-3 promotes osteosarcoma invasion and metastasis by inducing epithelial to mesenchymal transition and activating the Wnt/β-catenin signaling pathway |
| title_sort | retracted article fibulin 3 promotes osteosarcoma invasion and metastasis by inducing epithelial to mesenchymal transition and activating the wnt β catenin signaling pathway |
| url | https://doi.org/10.1038/s41598-017-06353-2 |
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